CAJ | 학술논문

目的评价瑞芬太尼预处理对老龄大鼠心肌缺血再灌注损伤的影响.方法雄性SD大鼠24只,15～ 18月龄,体重465 ～ 580 g,采用随机数字表法分为3组(n=8):假手术组(S组)、缺血再灌注组(I/R组)和瑞芬太尼预处理组(RP组).I/R组采用结扎左冠状动脉前降支30 min,再灌注120 min的方法制备心肌缺血再灌注损伤模型;RP组静脉输注瑞芬太尼10 μg·kg-1· min-120 min,洗脱10 min,然后制备心肌缺血再灌注损伤模型;S组只穿线,不结扎左冠状动脉前降支.分别于缺血前30 min和再灌注120 min时,测定血清乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)的活性.再灌注120 min时,取心肌组织测定心肌梗死体积,计算心肌梗死体积百分比,并观察超微结构.结果与S组比较,I/R组和RP组再灌注120 min时血清LDH和CK-MB的活性升高(P＜0.05);与I/R组比较,RP组再灌注120 min时血清LDH和CK-MB的活性降低,心肌梗死体积百分比减小(P＜0.05),病理学损伤减轻.结论瑞芬太尼预处理可减轻老龄大鼠心肌缺血再灌注损伤.
목적 평개서분태니예처리대노령대서심기결혈재관주손상적영향.방법 웅성SD대서24지,15～ 18월령,체중465 ～ 580 g,채용수궤수자표법분위3조(n=8):가수술조(S조)、결혈재관주조(I/R조)화서분태니예처리조(RP조).I/R조채용결찰좌관상동맥전강지30 min,재관주120 min적방법제비심기결혈재관주손상모형;RP조정맥수주서분태니10 μg·kg-1· min-120 min,세탈10 min,연후제비심기결혈재관주손상모형;S조지천선,불결찰좌관상동맥전강지.분별우결혈전30 min화재관주120 min시,측정혈청유산탈경매(LDH)화기산격매동공매(CK-MB)적활성.재관주120 min시,취심기조직측정심기경사체적,계산심기경사체적백분비,병관찰초미결구.결과 여S조비교,I/R조화RP조재관주120 min시혈청LDH화CK-MB적활성승고(P＜0.05);여I/R조비교,RP조재관주120 min시혈청LDH화CK-MB적활성강저,심기경사체적백분비감소(P＜0.05),병이학손상감경.결론 서분태니예처리가감경노령대서심기결혈재관주손상.
Objective To evaluate the effect of remifentanil preconditioning on myocardial ischemia-reperfusion (I/R) injury in aged rats.Methods Twenty-four healthy male Sprague-Dawley rats,aged 15-18 months,weighing 465-580 g,were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (group S),group I/R and remifentanil preconditioning group (group RP).The animals were anesthetized with intraperitoneal 10％ chloral hydrate 300 mg/kg.Myocardial I/R was induced by 30 min occlusion of anterior descending branch of left coronary artery followed by 120 min reperfusion in group I/R.In group RP,remifentanil 10 μg · kg-1 · min-1 was infused intravenously for 20 min followed by 10 min washout before myocardial I/R.In group S,the anterior descending branch was only exposed but not ligated.At 30 min before ligation and 120 min of reperfusion,the activities of serum lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) were determined.The hearts were removed at 120 min of reperfusion for determination of myocardial infarct size,the percentage of myocardial infarct size was calculated,and myocardial specimens were obtained for observing myocardial ultrastructure.Results Compared with group S,the activities of serum LDH and CK-MB were significantly increased at 120 min of reperfusion in I/R and RP groups.Compared with group I/R,the activities of serum LDH and CKMB were significantly decreased,the percentage of myocardial infarct size was decreased,and the pathological changes were attenuated in group RP.Conclusion Remifentanil preconditioning can attenuate myocardial I/R injury in aged rats.