CAJ | 학술논문

目的：探讨舒芬太尼在心肌缺血再灌注损伤中心肌保护效应的作用。方法：24只健康雄性SD大鼠（200～250g）随机分为4组，假手术（Sham)组,缺血再灌注（I/R）组,I/R+舒芬太尼组,Sham+舒芬太尼组。Sham组：只开胸但不结扎冠状动脉，静脉注射等量生理盐水；I/R组：开胸造MIRI模型（缺血30min，再灌注120min)注射等量生理盐水；I/R+S组：缺血前泵注舒芬太尼1μg/kg，泵注5min，停止5min，重复3次，总量共3μg/kg的预处理方式；Sham+S组：不结扎冠状动脉，相同方式注射舒芬太尼3μg/kg；再灌注120min后于腹主动脉抽取血液2ml，然后取心肌标本。血液标本检测CK-MB、LDH心肌酶。结果：四组雄性大鼠HR差异无统计学意义，I/R组与I/R+S组相比心肌酶（CK-MB值）从2538.9 U/L下降到1577.6U/L,差异有统计学意义（P<0.05）。结论：舒芬太尼在心肌缺血再灌注损伤中具有心肌保护作用。
목적：탐토서분태니재심기결혈재관주손상중심기보호효응적작용。방법：24지건강웅성SD대서（200～250g）수궤분위4조，가수술（Sham)조,결혈재관주（I/R）조,I/R+서분태니조,Sham+서분태니조。Sham조：지개흉단불결찰관상동맥，정맥주사등량생리염수；I/R조：개흉조MIRI모형（결혈30min，재관주120min)주사등량생리염수；I/R+S조：결혈전빙주서분태니1μg/kg，빙주5min，정지5min，중복3차，총량공3μg/kg적예처리방식；Sham+S조：불결찰관상동맥，상동방식주사서분태니3μg/kg；재관주120min후우복주동맥추취혈액2ml，연후취심기표본。혈액표본검측CK-MB、LDH심기매。결과：사조웅성대서HR차이무통계학의의，I/R조여I/R+S조상비심기매（CK-MB치）종2538.9 U/L하강도1577.6U/L,차이유통계학의의（P<0.05）。결론：서분태니재심기결혈재관주손상중구유심기보호작용。
Objective Explore sufentanil in myocardial ischemia-reperfusion injury of myocardial protective effect . Methods 24 healthy male SD rats (200~250 g) randomly divided into 4 groups:Sham group:only opening chest without ligation of coronary artery;sham+sufentanil group:sham operation with sufentanil pretreatment,I/R group:open chest with 30 min of ischemia and 120 min reperfusion of coronary artery;I/R+s group:30 min of ischemia and 120 min reperfusion of coronary artery with sufentanil pretreatment;After 120 min reperfusion, 2 ml blood withdraw from abdominal aorta . CK-MB and LDH levels were tested;Transmission electron microscopy was used to observe the morphological changes of myocardial cell.Results Four groups of HR has no statistically significant difference in the male rat(P>0.05) ,I/R group with S+IR group compared with myocardial enzyme (CK-MB values) fell to 1577.6 U/L from 2538.9 U/L and the differences reached significant level (P<0.05). Conclusions Sufentanil in myocardial ischemia-reperfusion injury of myocardial protection.