中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2015年
6期
459-461
,共3页
赵冬梅%郑遵荣%刘景瑶%毕惠梅%周玉珍%何晶晶%周珣
趙鼕梅%鄭遵榮%劉景瑤%畢惠梅%週玉珍%何晶晶%週珣
조동매%정준영%류경요%필혜매%주옥진%하정정%주순
荧光偏振检测方法%布鲁杆菌病%布病传统血清学试验
熒光偏振檢測方法%佈魯桿菌病%佈病傳統血清學試驗
형광편진검측방법%포로간균병%포병전통혈청학시험
Fluorescence polarization assay%Brucellosis%The conventional serological methods
目的 分析荧光偏振检测方法对布鲁杆菌病(简称布病)的诊断价值.方法 2013年8月至2014年4月,在黑龙江省农垦总局总医院选择布病患者240例作为病例组,正常体检人员287例作为对照组,抽取空腹静脉血4 ml,分离血清.采用荧光偏振检测方法(FPA)和4项传统血清学检测方法[包括虎红试验(RBPT)、试管凝集试验(SAT)、抗人免疫球蛋白试验(Coomb's试验)和半胱氨酸试验]进行检测,比较FPA与4项传统血清学检测方法的一致性、灵敏度和特异度.结果 FPA与4项传统血清学检测方法均有较好的一致性(Kappa值均≥0.75);FPA检测方法的敏感度(89.17%)高于RBPT (87.08%)、SAT (85.00%)、Coomb's试验(74.17%)和半胱氨酸试验(75.83%).FPA检测方法的特异度(94.08%)高于RBPT(89.90%)、Coomb's(81.53%),但低于SAT(98.95%)、半胱氨酸凝集试验(99.30%).结论 FPA可以作为人间布病的实验室筛查诊断方法,值得广泛推广使用.
目的 分析熒光偏振檢測方法對佈魯桿菌病(簡稱佈病)的診斷價值.方法 2013年8月至2014年4月,在黑龍江省農墾總跼總醫院選擇佈病患者240例作為病例組,正常體檢人員287例作為對照組,抽取空腹靜脈血4 ml,分離血清.採用熒光偏振檢測方法(FPA)和4項傳統血清學檢測方法[包括虎紅試驗(RBPT)、試管凝集試驗(SAT)、抗人免疫毬蛋白試驗(Coomb's試驗)和半胱氨痠試驗]進行檢測,比較FPA與4項傳統血清學檢測方法的一緻性、靈敏度和特異度.結果 FPA與4項傳統血清學檢測方法均有較好的一緻性(Kappa值均≥0.75);FPA檢測方法的敏感度(89.17%)高于RBPT (87.08%)、SAT (85.00%)、Coomb's試驗(74.17%)和半胱氨痠試驗(75.83%).FPA檢測方法的特異度(94.08%)高于RBPT(89.90%)、Coomb's(81.53%),但低于SAT(98.95%)、半胱氨痠凝集試驗(99.30%).結論 FPA可以作為人間佈病的實驗室篩查診斷方法,值得廣汎推廣使用.
목적 분석형광편진검측방법대포로간균병(간칭포병)적진단개치.방법 2013년8월지2014년4월,재흑룡강성농은총국총의원선택포병환자240례작위병례조,정상체검인원287례작위대조조,추취공복정맥혈4 ml,분리혈청.채용형광편진검측방법(FPA)화4항전통혈청학검측방법[포괄호홍시험(RBPT)、시관응집시험(SAT)、항인면역구단백시험(Coomb's시험)화반광안산시험]진행검측,비교FPA여4항전통혈청학검측방법적일치성、령민도화특이도.결과 FPA여4항전통혈청학검측방법균유교호적일치성(Kappa치균≥0.75);FPA검측방법적민감도(89.17%)고우RBPT (87.08%)、SAT (85.00%)、Coomb's시험(74.17%)화반광안산시험(75.83%).FPA검측방법적특이도(94.08%)고우RBPT(89.90%)、Coomb's(81.53%),단저우SAT(98.95%)、반광안산응집시험(99.30%).결론 FPA가이작위인간포병적실험실사사진단방법,치득엄범추엄사용.
Objective To analyze the diagnostic value of fluorescence polarization assay (FPA) for human brucellosis.Methods From April 2013 to August 2014,240 positive sera of brucella patients were collected as case group and 287 normal sera as control group in Heilongjiang Agricultural Reclamation Bureau General Hospital.Four milliliters of venous blood were withdrawn for separation of serum.Fluorescence polarization assay was used and the consistency,sensitivity and specificity were compared with those of four classical serological methods,including rose bengal plate agglutination test (RBPT),standard tube agglutination test (SAT),Coomb's test and cysteine test.Results The results of FPA were complied with those of the four conventional serological methods,Kappa ≥ 0.75.The sensitivity of FPA (89.17%) was higher than that of RBPT (87.08%),SAT (85.00%),Coomb's (74.17%) and cysteine test (75.83%).The specificity of FPA (94.08%) was higher than that of RBPT (89.90%) and Coomb's (81.53%),but lower than that of SAT (98.95%) and cysteine test (99.30%).Conclusion FPA can be used as a laboratory diagnostic method for human brucellosis,which is worthy to be widely used.