西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2015年
4期
462-466
,共5页
邵世滨%闵自信%郭源旭%王权成%孙梦瑶%韩燕%孙健
邵世濱%閔自信%郭源旭%王權成%孫夢瑤%韓燕%孫健
소세빈%민자신%곽원욱%왕권성%손몽요%한연%손건
sRNA%生物信息学%软骨生成%大鼠%Solexa 测序
sRNA%生物信息學%軟骨生成%大鼠%Solexa 測序
sRNA%생물신식학%연골생성%대서%Solexa 측서
small RNA (sRNA)%bioinformatics%chondrogenesis%rat%Solexa sequencing
目的：分析大鼠软骨形成过程中小分子 RNA(sRNA)表达谱变化及其基因功能,探讨软骨细胞增殖与分化的机制。方法取新生、断乳、性成熟3个时间节点的雌性 SD 大鼠股骨头软骨构建 sRNA 文库,Solexa 测序平台鉴定软骨组织全部 sRNA 序列表达,所得的全部清洁序列与 SD 大鼠基因组信息比对,并做生物信息学分析。结果3个文库筛选出与基因组序列完全匹配序列,分别对应着217921条(41.23%)、196650条(38.74%)、245436条(41.54%)unique sRNA 序列。长度为20~24 nt 的 sRNA 占比：d 0为71.94%、d 21为72.85%、d 42为86.39%；其中,长度为22 nt的 sRNA 约占清洁序列的一半。文库序列分布特征,符合高质量 sRNA 文库的特征。超过总数62%的清洁序列来自于成熟 miRNA 序列,但在3个文库中的占比却仅仅只有0.69%、0.78%和0.63%。约60%的unique sRNA 序列无法与 miRBase 20.0和 Rfam9.1匹配。结论3个 sRNA 文库的 miRNA 这种分布模式,可能暗示着有功能不同或者来源各异的 miRNA,参与了对骨骼发育和骨形成关键阶段软骨细胞增殖与分化的调控。
目的：分析大鼠軟骨形成過程中小分子 RNA(sRNA)錶達譜變化及其基因功能,探討軟骨細胞增殖與分化的機製。方法取新生、斷乳、性成熟3箇時間節點的雌性 SD 大鼠股骨頭軟骨構建 sRNA 文庫,Solexa 測序平檯鑒定軟骨組織全部 sRNA 序列錶達,所得的全部清潔序列與 SD 大鼠基因組信息比對,併做生物信息學分析。結果3箇文庫篩選齣與基因組序列完全匹配序列,分彆對應著217921條(41.23%)、196650條(38.74%)、245436條(41.54%)unique sRNA 序列。長度為20~24 nt 的 sRNA 佔比：d 0為71.94%、d 21為72.85%、d 42為86.39%；其中,長度為22 nt的 sRNA 約佔清潔序列的一半。文庫序列分佈特徵,符閤高質量 sRNA 文庫的特徵。超過總數62%的清潔序列來自于成熟 miRNA 序列,但在3箇文庫中的佔比卻僅僅隻有0.69%、0.78%和0.63%。約60%的unique sRNA 序列無法與 miRBase 20.0和 Rfam9.1匹配。結論3箇 sRNA 文庫的 miRNA 這種分佈模式,可能暗示著有功能不同或者來源各異的 miRNA,參與瞭對骨骼髮育和骨形成關鍵階段軟骨細胞增殖與分化的調控。
목적：분석대서연골형성과정중소분자 RNA(sRNA)표체보변화급기기인공능,탐토연골세포증식여분화적궤제。방법취신생、단유、성성숙3개시간절점적자성 SD 대서고골두연골구건 sRNA 문고,Solexa 측서평태감정연골조직전부 sRNA 서렬표체,소득적전부청길서렬여 SD 대서기인조신식비대,병주생물신식학분석。결과3개문고사선출여기인조서렬완전필배서렬,분별대응착217921조(41.23%)、196650조(38.74%)、245436조(41.54%)unique sRNA 서렬。장도위20~24 nt 적 sRNA 점비：d 0위71.94%、d 21위72.85%、d 42위86.39%；기중,장도위22 nt적 sRNA 약점청길서렬적일반。문고서렬분포특정,부합고질량 sRNA 문고적특정。초과총수62%적청길서렬래자우성숙 miRNA 서렬,단재3개문고중적점비각부부지유0.69%、0.78%화0.63%。약60%적unique sRNA 서렬무법여 miRBase 20.0화 Rfam9.1필배。결론3개 sRNA 문고적 miRNA 저충분포모식,가능암시착유공능불동혹자래원각이적 miRNA,삼여료대골격발육화골형성관건계단연골세포증식여분화적조공。
Objective To study the profiles and function of small RNA (sRNA)gene during chondrogenesis in rats so as to clarify the mechanisms of chondrocytes proliferation and differentiation.Methods All the sRNAs were identified from the female SD rats femoral head cartilages at three time points:at birth,ablactation and maturation,and three sRNA libraries were constructed.The Solexa sequencing and the bioinformatics analysis were employed to be blasted with the genomes of SD rats.Results The perfect match reads in the three libraries were screened out,which were correspondent to the 21 7 921 (41.23%),1 96 650 (38.74%)and 245 436 (41.54%)unique sRNA sequence,respectively.The percentages of 20-24 nt sRNA were 71.94% (d0),72.85% (d21),and 86.39%(d42).Half of clean sequences were 22 nt sRNA.The distribution characteristics of the reads were in line with the high-quality sRNA.More than 62% clean reads were from mature miRNA while the ratios in the three libraries were only 0.69%,0.78% and 0.63%.About 60% of the unique sRNA could not be matched with miRBase20.0 or Rfam9.1.Conclusion The distribution model of miRNA in the three libraries indicates that the miRNAs with different functions or from different sources are involved in the regulation of chondrocytes proliferation and differentiation in bone development and formation.
