中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2015年
15期
140-142
,共3页
苏家辉%林炳国%吴舜芳%蓝义琨
囌傢輝%林炳國%吳舜芳%藍義琨
소가휘%림병국%오순방%람의곤
参麦注射液%高效液相色谱法%人参皂苷Rg1%人参皂苷Re%人参皂苷Rf%人参皂苷Rb1
參麥註射液%高效液相色譜法%人參皂苷Rg1%人參皂苷Re%人參皂苷Rf%人參皂苷Rb1
삼맥주사액%고효액상색보법%인삼조감Rg1%인삼조감Re%인삼조감Rf%인삼조감Rb1
Shenmai Injection%HPLC%ginsenosides Rg1%ginsenoside Re%ginsenoside Rf%ginsenoside Rb1
目的:测定参麦注射液中主要有效成分人参皂苷Rg1、人参皂苷Re、人参皂苷Rf和人参皂苷Rb1的含量。方法:采用高效液相色谱法进行梯度洗脱,色谱柱为BDS Hypersil C18色谱柱(250 mm×4.6 mm,5.0μm),流动相为乙腈:水=25:75(0~40 min),35:65(40~70 min),检测波长为210 nm,进样量为15μL,流速为1.0 mL/min,柱温为35℃。结果:人参皂苷Rg1在0.3985~3.985μg(r=0.9998)、人参皂苷Re在0.4104~4.104μg(r=0.9997)、人参皂苷Rf在0.2998~2.998μg(r=0.9998)、人参皂苷Rb1在0.6042~6.042μg(r=0.9998)范围内具有稳定的线性关系;平均回收率分别为96.97%、98.47%、96.62%、98.55%(n=6), RSD 值分别为1.36%、1.22%、1.54%、1.67%。结论:高效液相色谱法用于参麦注射液有效成分的含量测定中,方法简单可靠,重复性好,含量测定准确,灵敏度高,可以作为参麦注射液含量测定和质量控制的方法之一。
目的:測定參麥註射液中主要有效成分人參皂苷Rg1、人參皂苷Re、人參皂苷Rf和人參皂苷Rb1的含量。方法:採用高效液相色譜法進行梯度洗脫,色譜柱為BDS Hypersil C18色譜柱(250 mm×4.6 mm,5.0μm),流動相為乙腈:水=25:75(0~40 min),35:65(40~70 min),檢測波長為210 nm,進樣量為15μL,流速為1.0 mL/min,柱溫為35℃。結果:人參皂苷Rg1在0.3985~3.985μg(r=0.9998)、人參皂苷Re在0.4104~4.104μg(r=0.9997)、人參皂苷Rf在0.2998~2.998μg(r=0.9998)、人參皂苷Rb1在0.6042~6.042μg(r=0.9998)範圍內具有穩定的線性關繫;平均迴收率分彆為96.97%、98.47%、96.62%、98.55%(n=6), RSD 值分彆為1.36%、1.22%、1.54%、1.67%。結論:高效液相色譜法用于參麥註射液有效成分的含量測定中,方法簡單可靠,重複性好,含量測定準確,靈敏度高,可以作為參麥註射液含量測定和質量控製的方法之一。
목적:측정삼맥주사액중주요유효성분인삼조감Rg1、인삼조감Re、인삼조감Rf화인삼조감Rb1적함량。방법:채용고효액상색보법진행제도세탈,색보주위BDS Hypersil C18색보주(250 mm×4.6 mm,5.0μm),류동상위을정:수=25:75(0~40 min),35:65(40~70 min),검측파장위210 nm,진양량위15μL,류속위1.0 mL/min,주온위35℃。결과:인삼조감Rg1재0.3985~3.985μg(r=0.9998)、인삼조감Re재0.4104~4.104μg(r=0.9997)、인삼조감Rf재0.2998~2.998μg(r=0.9998)、인삼조감Rb1재0.6042~6.042μg(r=0.9998)범위내구유은정적선성관계;평균회수솔분별위96.97%、98.47%、96.62%、98.55%(n=6), RSD 치분별위1.36%、1.22%、1.54%、1.67%。결론:고효액상색보법용우삼맥주사액유효성분적함량측정중,방법간단가고,중복성호,함량측정준학,령민도고,가이작위삼맥주사액함량측정화질량공제적방법지일。
Objective: To determine the content of the main active ingredients of ginsenosides Rg1, ginsenoside Re, ginsenoside Rf and ginsenoside Rb1 in Shenmai Injection. Methods: The high performance liquid chromatography was taken for gradient, elution column was BDS Hypersil C18 column (250 mm × 4.6 mm, 5.0 μm), the mobile phase of acetonitrile was water: 25: 75 (0 ~40 min), 35: 65 ( 40~70 min), the detection wavelength was 210 nm, the injection volume was 15 μL, the flow rate was 1.0 mL / min, and the column temperature was 35℃ . Results: Ginsenosides Rg1 had stable linear relationship in 0.3985 ~ 3.985μg (r = 0.9998), ginsenoside Re in 0.4104~4.104 μg (r = 0.9997), ginsenoside Rf in 0.2998~2.998μg (r = 0.9998), ginsenoside Rb1 at 0.6042~6.042 μg (r = 0.9998), and the average recovery rates were 96.97%, 98.47%, 96.62%, 98.55% (n = 6), RSD values were 1.36%, 1.22%, 1.54%, 1.67 %. Conclusion: HPLC in the application of the determination of the active ingredient in Shenmai injection, the method is simple, reliable, reproducible and accurate. It is of high sensitivity, which can be used as one of the methods for the determination and quality control for Shenmai Injection.