CAJ | 학술논문

目的：研究人成骨细胞（ hFOB 1．19）在不同铁离子状态下RANKL／OPG基因及蛋白的表达。方法成骨细胞株（hFOB1．19）在DMEM／F－12培养基培养传代至第3代后，用不同终浓度枸橼酸铁铵（50、100、200μmol／L）加入细胞培养基中干预24 h，用RT－PCR方法和免疫印迹法（ Western Blot）检测干预后成骨细胞的RANKL、OPG mRNA和蛋白表达并计算RANKL／OPG比率。结果 RT－PCR检测结果显示，对照组、50、100、200μmol／L组RANKL／OPG mRNA表达比分别为0．56±0．13、0．58±0．01、0．69±0．01、1．84±0．92；Western blot结果显示，对照组、50、100、200μmol／L组RANKL／OPG蛋白表达比分别为0．82±0．66、0．82±0．64、1．09±0．11、1．25±0．14。统计学分析显示，在mRNA和蛋白水平，100和200μmol／L浓度的枸橼酸铁铵干预后RANKL／OPG比值明显高于对照组（ P＜0．05），50μmol／L枸橼酸铁干预后与对照组差异无统计学意义。结论不同浓度枸橼酸铁铵可以影响人成骨细胞RANKL／OPG基因及蛋白的表达，进而可能影响骨形成和骨吸收。
목적：연구인성골세포（ hFOB 1．19）재불동철리자상태하RANKL／OPG기인급단백적표체。방법성골세포주（hFOB1．19）재DMEM／F－12배양기배양전대지제3대후，용불동종농도구연산철안（50、100、200μmol／L）가입세포배양기중간예24 h，용RT－PCR방법화면역인적법（ Western Blot）검측간예후성골세포적RANKL、OPG mRNA화단백표체병계산RANKL／OPG비솔。결과 RT－PCR검측결과현시，대조조、50、100、200μmol／L조RANKL／OPG mRNA표체비분별위0．56±0．13、0．58±0．01、0．69±0．01、1．84±0．92；Western blot결과현시，대조조、50、100、200μmol／L조RANKL／OPG단백표체비분별위0．82±0．66、0．82±0．64、1．09±0．11、1．25±0．14。통계학분석현시，재mRNA화단백수평，100화200μmol／L농도적구연산철안간예후RANKL／OPG비치명현고우대조조（ P＜0．05），50μmol／L구연산철간예후여대조조차이무통계학의의。결론불동농도구연산철안가이영향인성골세포RANKL／OPG기인급단백적표체，진이가능영향골형성화골흡수。
Objective To investigate the effects of Ferric Ammonium Citrate (FAC) on gene and protein ex-pression of RANKL and OPG of human osteoblasts.Methods After human osteoblasts were cultured in the medium, cells were supplemented with FAC at the final concentration of 0, 50, 100, and 200 μmol/L.After treatment with FAC for 24 h, the mRNA and protein expression of RANKL and OPG both secreted by osteoblasts were detected by reverse transcription polymerase chain reaction ( RT-PCR) and Western blot respectively, and calculated the RANKL/OPG ratio afterwards.Results The mRNA expression of RANKL/OPG ratio in human osteoblast hFOB1.19 cells of control, 50, 100, 200 μmol/L groups were (0.56 ±0.13), (0.58 ±0.01), (0.69 ±0.01), (1.84 ±0.92) respectively; the protein expression of RANKL/OPG ratio in human osteoblast hFOB1.19 cells of control, 50, 100, 200 μmol/L groups were (0.82 ±0.66), (0.82 ±0.64), (1.09 ±0.11), (1.25 ±0.14) respectively.Statistical analysis revealed that FAC treatment at 100μmol/L and 200μmol/L significantly increased the expression of RANKL/OPG compared to control group;whereas FAC treatment at 50 μmol/L did not significantly alter the mRNA and protein expression of RANKL/OPG.Conclusion These results indicated that different concentration of FAC affected the expression of RANKL/OPG in human osteoblasts, which might further change bone formation and bone resorption.