CAJ | 학술논문

目的评价Src激酶在内毒素血症小鼠肝损伤中的作用.方法雌性BABL/c小鼠48只,体重15～20 g,3～4月龄,采用随机数字表法分为3组(n=16):对照组(C组)、内毒素血症组(LPS组)和Src激酶抑制剂PP2组(PP2组).LPS组和PP2组腹腔注射脂多糖(LPS) 20 mg/kg,C组给予等容量PBS,2h后PP2组腹腔注射PP2 1 mg/kg.于注射LPS或PBS后6h取8只大鼠,经腹主动脉采集血样,测定血清碱性磷酸酶(ALP)水平;随后处死大鼠,取肝组织,检测核因子E2相关因子2(Nrf2)水平、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及髓过氧化物酶(MPO)活性;于注射LPS或PBS后24 h处死8只大鼠,取肝组织,行病理学观察.结果与C组比较,LPS组和PP2组血清ALP、肝组织MDA和MPO的水平升高,SOD和Nrf2的水平降低(P＜0.05);与LPS组比较,PP2组血清ALP、肝组织MDA和MPO的水平降低,SOD和Nrf2的水平升高(P＜0.05).PP2组小鼠肝组织病理学损伤较LPS组减轻.结论 Src激酶参与了内毒素血症诱发小鼠肝损伤的过程.
목적 평개Src격매재내독소혈증소서간손상중적작용.방법 자성BABL/c소서48지,체중15～20 g,3～4월령,채용수궤수자표법분위3조(n=16):대조조(C조)、내독소혈증조(LPS조)화Src격매억제제PP2조(PP2조).LPS조화PP2조복강주사지다당(LPS) 20 mg/kg,C조급여등용량PBS,2h후PP2조복강주사PP2 1 mg/kg.우주사LPS혹PBS후6h취8지대서,경복주동맥채집혈양,측정혈청감성린산매(ALP)수평;수후처사대서,취간조직,검측핵인자E2상관인자2(Nrf2)수평、초양화물기화매(SOD)활성、병이철(MDA)함량급수과양화물매(MPO)활성;우주사LPS혹PBS후24 h처사8지대서,취간조직,행병이학관찰.결과 여C조비교,LPS조화PP2조혈청ALP、간조직MDA화MPO적수평승고,SOD화Nrf2적수평강저(P＜0.05);여LPS조비교,PP2조혈청ALP、간조직MDA화MPO적수평강저,SOD화Nrf2적수평승고(P＜0.05).PP2조소서간조직병이학손상교LPS조감경.결론 Src격매삼여료내독소혈증유발소서간손상적과정.
Objective To evaluate the role of Src kinase in liver injury in endotoxemic mice.Methods Forty-eight female BABL/c mice,aged 3-4 months,weighing 15-20 g,were randomly divided into 3 groups (n =16 each) using a random number table:control group (C group),endotoxemia group (lipopolysaccharide (LPS) group) and Src kinase inhibitor PP2 group (PP2 group).Endotoxemia was induced by intraperitoneal LPS 20 mg/kg in LPS and PP2 groups,while the equal volume of PBS was given in group C.In PP2 group,PP2 1 mg/kg was injected intraperitoneally at 2 h after LPS administration.At 6 h after LPS or PBS injection,8 mice in each group were chosen,and blood samples were collected from the abdominal aorta for determination of the serum levels of alkaline phosphatase (ALP).The mice were then sacrificed and livers were removed for determination of nuclear factor E2-related factor 2 (Nrf2) level,superoxide dismutase (SOD) activity,malondialdehyde (MDA) content and myeloperoxidase (MPO) activity in liver tissues.The other 8 mice in each group were sacrificed at 24 h after LPS or PBS injection,and the livers were harvested for examination of pathological changes.Results Compared with C group,the serum levels of ALP and MDA content and MPO activity in liver tissues were significantly increased,and SOD activity and Nrf2 levels in liver tissues were decreased in LPS and PP2 groups.Compared with LPS group,the serum levels of ALP and MDA content and MPO activity in liver tissues were significantly decreased,and SOD activity and Nrf2 levels in liver tissues were increased in PP2 group.The pathological changes of liver tissues were significantly attenuated in PP2 group as compared with LPS group.Conclusion Src kinase is involved in endotoxemia-induced liver injury in mice.