广东医学
廣東醫學
엄동의학
GUNAGDONG MEDICAL JOURNAL
2015年
11期
1638-1641
,共4页
张崇%屈艳%尚校军%白素平
張崇%屈豔%尚校軍%白素平
장숭%굴염%상교군%백소평
蓝萼甲素%γ-环糊精%p53%S180肿瘤
藍萼甲素%γ-環糊精%p53%S180腫瘤
람악갑소%γ-배호정%p53%S180종류
glaucocalyxin A%γ-cyclodextrin%p53%S180 tumor
目的：探讨蓝萼甲素γ－环糊精包合物（ GLA－γ－CD）对荷瘤小鼠S180移植瘤生长及凋亡的影响。方法制备小鼠S180皮下移植瘤模型，随机分为5组，每组10只。对照组给予生理盐水，环磷酰胺组给予环磷酰胺10 mg／kg，GLA－γ－CD高、中、低剂量组分别给予GLA－γ－CD 80、40、20 mg／kg，均腹腔注射给药12 d，观察抑瘤作用，TUNEL法检测细胞凋亡，免疫组化法及实时荧光定量PCR法分别检测突变型p53蛋白及mRNA的表达。结果与对照组比较，GLA－γ－CD各剂量组的瘤重均明显降低（P＜0.01，P＜0.05），其中GLA－γ－CD高剂量组抑瘤率达57.26％，肿瘤细胞凋亡指数明显提高（ P＜0.01），突变型 p53蛋白及 mRNA 表达显著降低（ P＜0.05），且与环磷酰胺组作用相当。结论 GLA－γ－CD能明显抑制S180肿瘤生长，其机制可能与减少突变型p53的表达，从而诱导细胞凋亡有关；并且GLA－γ－CD在发挥抗肿瘤同时毒性作用不大，具有较好的开发前景。
目的：探討藍萼甲素γ－環糊精包閤物（ GLA－γ－CD）對荷瘤小鼠S180移植瘤生長及凋亡的影響。方法製備小鼠S180皮下移植瘤模型，隨機分為5組，每組10隻。對照組給予生理鹽水，環燐酰胺組給予環燐酰胺10 mg／kg，GLA－γ－CD高、中、低劑量組分彆給予GLA－γ－CD 80、40、20 mg／kg，均腹腔註射給藥12 d，觀察抑瘤作用，TUNEL法檢測細胞凋亡，免疫組化法及實時熒光定量PCR法分彆檢測突變型p53蛋白及mRNA的錶達。結果與對照組比較，GLA－γ－CD各劑量組的瘤重均明顯降低（P＜0.01，P＜0.05），其中GLA－γ－CD高劑量組抑瘤率達57.26％，腫瘤細胞凋亡指數明顯提高（ P＜0.01），突變型 p53蛋白及 mRNA 錶達顯著降低（ P＜0.05），且與環燐酰胺組作用相噹。結論 GLA－γ－CD能明顯抑製S180腫瘤生長，其機製可能與減少突變型p53的錶達，從而誘導細胞凋亡有關；併且GLA－γ－CD在髮揮抗腫瘤同時毒性作用不大，具有較好的開髮前景。
목적：탐토람악갑소γ－배호정포합물（ GLA－γ－CD）대하류소서S180이식류생장급조망적영향。방법제비소서S180피하이식류모형，수궤분위5조，매조10지。대조조급여생리염수，배린선알조급여배린선알10 mg／kg，GLA－γ－CD고、중、저제량조분별급여GLA－γ－CD 80、40、20 mg／kg，균복강주사급약12 d，관찰억류작용，TUNEL법검측세포조망，면역조화법급실시형광정량PCR법분별검측돌변형p53단백급mRNA적표체。결과여대조조비교，GLA－γ－CD각제량조적류중균명현강저（P＜0.01，P＜0.05），기중GLA－γ－CD고제량조억류솔체57.26％，종류세포조망지수명현제고（ P＜0.01），돌변형 p53단백급 mRNA 표체현저강저（ P＜0.05），차여배린선알조작용상당。결론 GLA－γ－CD능명현억제S180종류생장，기궤제가능여감소돌변형p53적표체，종이유도세포조망유관；병차GLA－γ－CD재발휘항종류동시독성작용불대，구유교호적개발전경。
Objective To investigate the effects of glaucocalyxin A included by γ-cyclodextrin ( GLA-γ-CD) on transplanted S180 tumor growth and apoptosis.Methods The transplanted S180 tumor models were established by implanting S180 cells into mice.The mice were randomly divided into 5 groups (n=10), control group (given with physio-logical saline), cyclophosphamide group (given with 10 mg/kg cyclophosphamide), GLA-γ-CD high, middle and low dose groups (given with 80, 40, and 20 mg/kg GLA-γ-CD, respectively).All drugs were given as intraperitoneal in-jection every day for 12 days.The effect of GLA-γ-CD on tumor growth was observed .Cell apoptosis was determined by TUNEL.The protein and mRNA expression of mutant p 53 was measured by immunohistochemistry and real -time fluo-rescence quantitative RT-PCR, respectively.Results Compared with control group , the tumor weight in each GLA -γ-CD group was significantly reduced (P<0.01, P<0.05).The tumor inhibition rate against S180 of GLA-γ-CD high dose group was 57.26%, and increased apoptosis index and down -regulation of mutant p 53 protein and mRNA (P<0.01) were observed, which were close to that of the cyclophosphamide group .Conclusion GLA-γ-CD can in-duce apoptosis and inhibit growth of tumor cells , which may be associated with regulating the expression of mutant p 53, with little cytotoxicity .
