CAJ | 학술논문

目的通过与乌司他丁比较,评价大黄对脓毒症大鼠肠黏膜损伤的影响.方法健康SD大鼠84只,3月龄,体重200～ 330 g,雌雄各半,采用随机数字表法,将其分为5组:对照组(C组,n=6)、假手术组(S组,n=6)、脓毒症组(Sep组,n=24)、大黄组(R组,n=24)和乌司他丁组(U组,n=24).采用盲肠结扎穿孔的方法制备大鼠脓毒症模型.R组大黄粉1.2 g/100 g溶于室温生理盐水中泡制3～4h后取全部滤液,约2～3 ml,灌胃1次/12 h,72 h后制备脓毒症模型;U组腹腔注射乌司他丁50 000 U/kg,加生理盐水至2 ml,1次/24 h,72 h后制备脓毒症模型.Sep组、R组和U组于结扎盲肠后6、12、24和48 h(T1-4)时采集眼眶静脉丛血样,采用ELSIA法测定血浆二胺氧化酶(DAO)活性.结果与C组和S组比较,Sep组、R组和U组T1-4时血浆DAO活性升高(P＜0.05);与Sep组比较,R组和U组T34时血浆DAO活性降低(P＜0.05);R组和U组T1-4时血浆DAO活性差异无统计学意义(P＞0.05).结论大黄减轻脓毒症大鼠肠黏膜损伤的效应与乌司他丁相似.
목적 통과여오사타정비교,평개대황대농독증대서장점막손상적영향.방법 건강SD대서84지,3월령,체중200～ 330 g,자웅각반,채용수궤수자표법,장기분위5조:대조조(C조,n=6)、가수술조(S조,n=6)、농독증조(Sep조,n=24)、대황조(R조,n=24)화오사타정조(U조,n=24).채용맹장결찰천공적방법제비대서농독증모형.R조대황분1.2 g/100 g용우실온생리염수중포제3～4h후취전부려액,약2～3 ml,관위1차/12 h,72 h후제비농독증모형;U조복강주사오사타정50 000 U/kg,가생리염수지2 ml,1차/24 h,72 h후제비농독증모형.Sep조、R조화U조우결찰맹장후6、12、24화48 h(T1-4)시채집안광정맥총혈양,채용ELSIA법측정혈장이알양화매(DAO)활성.결과 여C조화S조비교,Sep조、R조화U조T1-4시혈장DAO활성승고(P＜0.05);여Sep조비교,R조화U조T34시혈장DAO활성강저(P＜0.05);R조화U조T1-4시혈장DAO활성차이무통계학의의(P＞0.05).결론 대황감경농독증대서장점막손상적효응여오사타정상사.
Objective To evaluate the effect of rhubarb on intestinal mucosal damage in septic rats by comparing with ulinastatin.Methods A total of 84 healthy Sprague-Dawley rats (half male,half female),aged 3 months,weighing 200-330 g,were randomly divided into 5 groups according to the random number table:control group (group C,n =6),sham operation group (group S,n =6),sepsis group (group Sep),rhubarb group (group R,n=24) and ulinastatin group (group U,n=24).Sepsis was induced by cecum ligation and puncture.In group R,rhubarb 1.2 g/100 g was dissolved in normal saline at room temperature,3 and 4 h later the filtrate about 2-3 ml was obtained and injected through a gastric tube into stomach once every 12 h,and 72 h later sepsis was induced.In group U,ulinastatin 50 000 U/kg (in 2 ml of normal saline) was injected once every 24 h,and 72 h later sepsis was induced.In Sep,R and U groups,at 6,12,24 and 48 h after ligation (T1 4),blood samples were collected from the orbital venous plexus for determination of plasma diamine oxidase (DAO) activity.Results The activity of plasma DAO was significantly higher at T1-4 in Sep,R and U groups than in C and S groups.The activity of plasma DAO was significantly lower at T3,4 in R and U groups than in Sep group.There was no statistical difference in the plasma DAO activity between R group and U group.Conclusion Rhubarb can reduce intestinal mucosal damage in septic rats,which is similar to that of ulinastatin.