中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2015年
6期
14-17
,共4页
潘金春%任柏桦%闵凡贵%陈瑞爱%王希龙%王林川%王凤国%罗书明%叶健聪%刘玲%黄鹏华
潘金春%任柏樺%閔凡貴%陳瑞愛%王希龍%王林川%王鳳國%囉書明%葉健聰%劉玲%黃鵬華
반금춘%임백화%민범귀%진서애%왕희룡%왕림천%왕봉국%라서명%협건총%류령%황붕화
小型猪%高致病性猪繁殖与呼吸综合征病毒%敏感性筛选
小型豬%高緻病性豬繁殖與呼吸綜閤徵病毒%敏感性篩選
소형저%고치병성저번식여호흡종합정병독%민감성사선
Minipigs%Porcine reproductive and respiratory syndrome,PRRS%Highly pathogenic PRRS virus,HP-PRRSV%Susceptibility screening
目的：筛选对高致病性猪繁殖与呼吸综合征病毒（ HP-PRRSV ）敏感的小型猪品系，以用于HP-PRRS活疫苗的评价。方法本研究选择蓝塘猪、蕨麻小型猪、巴马小型猪、五指山小型猪（白系）接种PRRSV NVDC-JXA1株强毒，并以本地二元杂猪作为对照。攻毒后每天观察动物精神、食欲、死亡等情况，死亡动物进行剖检，并采取肺组织做RT-PCR检测病毒，连续观察5周，试验结束时采取存活动物血清分别检测PRRSV抗体。结果攻毒后各组都有动物出现精神沉郁、食欲下降、死亡等现象，死亡动物RT-PCR 检测都显示为阳性，存活动物PRRSV抗体检测阳性。从死亡率看，巴马小型猪和五指山小型猪白系对PRRSV NVDC-JXA1株强毒的敏感性最高，并且敏感性超过了对照组二元杂猪。结论巴马小型猪和五指山小型猪白系对HP-PRRSV敏感，可用于HP-PRRS活疫苗的检验。
目的：篩選對高緻病性豬繁殖與呼吸綜閤徵病毒（ HP-PRRSV ）敏感的小型豬品繫，以用于HP-PRRS活疫苗的評價。方法本研究選擇藍塘豬、蕨痳小型豬、巴馬小型豬、五指山小型豬（白繫）接種PRRSV NVDC-JXA1株彊毒，併以本地二元雜豬作為對照。攻毒後每天觀察動物精神、食欲、死亡等情況，死亡動物進行剖檢，併採取肺組織做RT-PCR檢測病毒，連續觀察5週，試驗結束時採取存活動物血清分彆檢測PRRSV抗體。結果攻毒後各組都有動物齣現精神沉鬱、食欲下降、死亡等現象，死亡動物RT-PCR 檢測都顯示為暘性，存活動物PRRSV抗體檢測暘性。從死亡率看，巴馬小型豬和五指山小型豬白繫對PRRSV NVDC-JXA1株彊毒的敏感性最高，併且敏感性超過瞭對照組二元雜豬。結論巴馬小型豬和五指山小型豬白繫對HP-PRRSV敏感，可用于HP-PRRS活疫苗的檢驗。
목적：사선대고치병성저번식여호흡종합정병독（ HP-PRRSV ）민감적소형저품계，이용우HP-PRRS활역묘적평개。방법본연구선택람당저、궐마소형저、파마소형저、오지산소형저（백계）접충PRRSV NVDC-JXA1주강독，병이본지이원잡저작위대조。공독후매천관찰동물정신、식욕、사망등정황，사망동물진행부검，병채취폐조직주RT-PCR검측병독，련속관찰5주，시험결속시채취존활동물혈청분별검측PRRSV항체。결과공독후각조도유동물출현정신침욱、식욕하강、사망등현상，사망동물RT-PCR 검측도현시위양성，존활동물PRRSV항체검측양성。종사망솔간，파마소형저화오지산소형저백계대PRRSV NVDC-JXA1주강독적민감성최고，병차민감성초과료대조조이원잡저。결론파마소형저화오지산소형저백계대HP-PRRSV민감，가용우HP-PRRS활역묘적검험。
Objective To screen strains of minipigs sensitive to highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) for evaluation of HP-PRRS live vaccine.Methods Lantang pigs, Juema, Bama and Wuzhishan ( white) minipigs were inoculated with virulent strain NVDC-JXA1 of PRRSV, and local binary hybrid pigs were used as control.The animals were continuously observed for 5 weeks on mental status, appetite, survival, etc.after inoculation of virus.The dead pigs were autopsied and the lung tissue samples were collected for detecting virus by RT-PCR.By the end of the experiment, serum of survival animals were collected for detecting PRRSV antibody by ELISA assay.Result The animals showed depression, anorexia, and other clinical signs and death in each group after inoculation.Meanwhile, the testing results were all positive in the RT-PCR and ELISA detection.Bama and Wuzhishan ( white) minipigs were the most sensitive to virulent strain NVDC-JXA1 of PRRSV regarding mortality rate.Conclusions Bama and Wuzhishan ( white) minipigs are sensitive to HP-PRRSV, and can be used for the inspection of HP-PRRS live vaccine.
