CAJ | 학술논문

目的：研究淫羊藿苷对阿尔茨海默病（Alzheimer disease ，AD ）模型大鼠行为学及海马神经元超微结构的影响，探讨淫羊藿苷治疗AD的作用机理。方法经水迷宫筛选的18只雄性Wistar大鼠随机分为正常对照组，模型组，淫羊藿苷治疗组。模型组、淫羊藿苷治疗组大鼠给予腹腔注射脂多糖（L PS ）制作实验性AD炎症模型，采用Morris水迷宫实验观察大鼠学习记忆能力，实验结束后开颅取脑，电镜下观察各组大鼠脑组织海马神经元超微结构的变化。结果定位航行实验中，记录各组大鼠逃避潜伏期，模型组与正常对照组大鼠比较差异有统计学意义（P＜0．05），治疗组与模型组大鼠比较差异有统计学意义（P＜0．05）；空间探索实验中，记录各组大鼠靶象限活动时间百分比，模型组与正常对照组大鼠比较差异有统计学意义（P＜0．05），治疗组与模型组大鼠比较差异有统计学意义（P＜0．05）。对照组海马神经元超微结构正常，与对照组比较，模型组大鼠海马神经元细胞胞膜节段性断裂、不清，核染色质聚集成块、边集；线粒体肿胀，内有空泡形成；高尔基体肿胀；内质网扩张；淫羊藿苷治疗组大鼠神经细胞上述病理变化有所减轻。结论经Morris水迷宫行为学检测，腹腔注射LPS制作AD炎症模型成功；淫羊藿苷能够改善AD模型大鼠的学习记忆能力，对模型大鼠海马神经元超微结构损伤具有保护作用。
목적：연구음양곽감대아이자해묵병（Alzheimer disease ，AD ）모형대서행위학급해마신경원초미결구적영향，탐토음양곽감치료AD적작용궤리。방법경수미궁사선적18지웅성Wistar대서수궤분위정상대조조，모형조，음양곽감치료조。모형조、음양곽감치료조대서급여복강주사지다당（L PS ）제작실험성AD염증모형，채용Morris수미궁실험관찰대서학습기억능력，실험결속후개로취뇌，전경하관찰각조대서뇌조직해마신경원초미결구적변화。결과정위항행실험중，기록각조대서도피잠복기，모형조여정상대조조대서비교차이유통계학의의（P＜0．05），치료조여모형조대서비교차이유통계학의의（P＜0．05）；공간탐색실험중，기록각조대서파상한활동시간백분비，모형조여정상대조조대서비교차이유통계학의의（P＜0．05），치료조여모형조대서비교차이유통계학의의（P＜0．05）。대조조해마신경원초미결구정상，여대조조비교，모형조대서해마신경원세포포막절단성단렬、불청，핵염색질취집성괴、변집；선립체종창，내유공포형성；고이기체종창；내질망확장；음양곽감치료조대서신경세포상술병리변화유소감경。결론경Morris수미궁행위학검측，복강주사LPS제작AD염증모형성공；음양곽감능구개선AD모형대서적학습기억능력，대모형대서해마신경원초미결구손상구유보호작용。
Objective To observe the influence of icariin on ultrastructural changes of hippocampus in Alzheimer disease rat and explore the mechanism of icariin to AD .Methods After the Morris water maze test , 1 8 Wistar rats were randomly divided into three groups:normal control group ,model group ,Icariin treatment group .The i .P .Injection of LPS for rats of model group and Icariin treatment group were administrated ,Mor-ris water maze test was used to evaluate the memory dysfunction and electron microscope was used to observe the ultrastructure of hippocampus neuron .Results In Water maze test ,data of the escape latency of rats in each group was recorded .there was a significant difference between the model group and the normal control group(P<0 .05 ) ,as well as the same result between the treatment group and the model group(P< 0 .05 );In spatial probe test ,average time percentage spent on the target quadrant of model group was significantly decreased(P<0 .05 )while the time were significantly increased of treatment group than those of model group(P<0 .05 );In the model group ,the cellular membrane was irregular and discontinue ,nuclear membrane was cloudiness1 .The nuclear chromatin was aggregate .The mitochondria was oncootic and vacuolate .The golgi apparatus was on-cootic;The endoplasmic reticulum was dilative .These ultrastructural pathological changes in the treatment group were not apparent .Conclusion In this experiment ,AD model mice which administrated LPS appeared decrease in memory dysfunction ,it indicated that a successful AD rat model has been made .Icariin is beneficial for im-proving the memory dysfunction ,attenuating the neural damage of AD model rat .