CAJ | 학술논문

目的：研制载万古霉素PLGA／CS缓释复式微球，考察微球的体外释放性能。方法应用复乳－固化挥发法制备载盐酸万古霉素 PLGA／CS 复式微球。利用扫描电镜（SEM ）及激光粒度分析仪进行形貌观测，采用紫外分光光度法测定盐酸万古霉素含量，计算载药率和包埋率；评价载药微球的体外释放行为，测定药物在微球中的稳定性，通过体外药物释放实验考察复合微球化处理对盐酸万古霉素溶出度的影响，用抑菌实验检测盐酸万古霉素的抗菌能力。结果以聚乙烯醇为乳化剂制备载万古霉素PLGA／CS缓释复式微球，微球表面光滑、无孔隙，粒径在（252．3±5．3）μm ，平均载药量在（3．12±0．78）％。平均包埋率（33．57±2．17）％，在盐酸万古霉素PLGA／CS缓释微球具有明显的药物释放作用，体外释放90 d的药物累计释放高达80％以上。结论载盐酸万古霉素 PLGA／CS 微球性质稳定，释放周期比较长，抑菌效果明显，符合理想的局部抗感染应用要求。
목적：연제재만고매소PLGA／CS완석복식미구，고찰미구적체외석방성능。방법응용복유－고화휘발법제비재염산만고매소 PLGA／CS 복식미구。이용소묘전경（SEM ）급격광립도분석의진행형모관측，채용자외분광광도법측정염산만고매소함량，계산재약솔화포매솔；평개재약미구적체외석방행위，측정약물재미구중적은정성，통과체외약물석방실험고찰복합미구화처리대염산만고매소용출도적영향，용억균실험검측염산만고매소적항균능력。결과이취을희순위유화제제비재만고매소PLGA／CS완석복식미구，미구표면광활、무공극，립경재（252．3±5．3）μm ，평균재약량재（3．12±0．78）％。평균포매솔（33．57±2．17）％，재염산만고매소PLGA／CS완석미구구유명현적약물석방작용，체외석방90 d적약물루계석방고체80％이상。결론재염산만고매소 PLGA／CS 미구성질은정，석방주기비교장，억균효과명현，부합이상적국부항감염응용요구。
Objective Prepare load Vancomycin hydrochloride PLGA/CS Duplex microspheres ,investi-gated Duplex microsphere external release performance .Methods Prepare the Vancomycin hydrochloride -loaded Poly lactic acid glycolic acid copolymer Vancomycin by the multiple emulsion ,ese the SEM and HPLC to observe .use the UV spectrophotometer to measuring the PLGA content ,calculate particle size distribution ,en-capsulation efficiency .Evaluate the Duplex microspheres in vitro release ,determine the drug Stability in the mi-crospheres .Results Use the Antibacterial experiment to test the Vancomycin hydrochloride active against bac-teria .Experimental study released composite microspheres of treatment on Vancomycin hydrochloride by disso-lution in vitro drug ,use the polyvinyl alcohol as emulsifier to Prepare load Vancomycin hydrochloride PLGA/CS duplex microspheres ,the surface is smooth and no gap ,average particle size is(2 5 2 .3 ± 5 .3 )mm ,the average drug loading was (3 .1 2 ± 0 .7 8 )% ,with an average encapsulation efficiency (3 3 .5 7 ± 2 .1 7 )% .Vancomycin hydrochloride PLGA/CS Duplex microspheres have obvious drug release action .In vitro drug release 9 0 d cumu-lative release up to 8 0% .Conclusion Prepare load Vancomycin hydrochloride PLGA/CS Duplex microspheres quality is stable ,Release cycle is more long ,antibacterial effect is obvious ,With the ideal of topical anti-infec-tive applications .