医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2015年
7期
946-949
,共4页
连翘配方颗粒%连翘酯苷A%连翘苷%色谱法,高效液相%图谱,指纹
連翹配方顆粒%連翹酯苷A%連翹苷%色譜法,高效液相%圖譜,指紋
련교배방과립%련교지감A%련교감%색보법,고효액상%도보,지문
Forsythia suspensa dispensing granule%Forsythoside A%Forsythin%Chromatography,high performance liquid%Chromatogram fingerprint
目的：建立连翘配方颗粒的高效液相色谱( HPLC)指纹图谱。方法采用高效液相色谱梯度洗脱方法,测定10批连翘配方颗粒中连翘苷含量,建立指纹图谱；色谱柱为Waters SunFire C18(4.6 mm×250 mm,5μm)；流动相：乙腈(A)-0.4%醋酸溶液(B),检测波长277 nm,柱温30℃,流速1.0 mL·min-1。结果连翘配方颗粒含13个共有色谱峰,相似度均>0.999,有2个已知峰分别为连翘酯苷A和连翘苷。结论连翘配方颗粒HPLC指纹图谱稳定,操作简便,可为其质量控制提供参考。
目的：建立連翹配方顆粒的高效液相色譜( HPLC)指紋圖譜。方法採用高效液相色譜梯度洗脫方法,測定10批連翹配方顆粒中連翹苷含量,建立指紋圖譜；色譜柱為Waters SunFire C18(4.6 mm×250 mm,5μm)；流動相：乙腈(A)-0.4%醋痠溶液(B),檢測波長277 nm,柱溫30℃,流速1.0 mL·min-1。結果連翹配方顆粒含13箇共有色譜峰,相似度均>0.999,有2箇已知峰分彆為連翹酯苷A和連翹苷。結論連翹配方顆粒HPLC指紋圖譜穩定,操作簡便,可為其質量控製提供參攷。
목적：건립련교배방과립적고효액상색보( HPLC)지문도보。방법채용고효액상색보제도세탈방법,측정10비련교배방과립중련교감함량,건립지문도보；색보주위Waters SunFire C18(4.6 mm×250 mm,5μm)；류동상：을정(A)-0.4%작산용액(B),검측파장277 nm,주온30℃,류속1.0 mL·min-1。결과련교배방과립함13개공유색보봉,상사도균>0.999,유2개이지봉분별위련교지감A화련교감。결론련교배방과립HPLC지문도보은정,조작간편,가위기질량공제제공삼고。
Objective To establish the fingerprint of Forsythia suspensa dispensing granule by HPLC. Methods The total of 10 samples of Forsythia suspensa were analyzed by HPLC on a Waters SunFire C18 column(4. 6 mm×250 mm,5 μm) with mobile phase of A:acetonitrile and mobile phase B:0. 4% acetic acid solution as gradient elution, with flow rate at 1. 0 mL·min-1 , wavelength at 277 nm and column temperature at 30 ℃. Results There were 13 characteristic common peaks in the Forsythia suspensa dispensing granule, in which two peaks identified as forsythoside A and forsythin, the similarities were more than 0. 999. Conclusion The HPLC fingerprint of Forsythia suspensa dispensing granule is stable, and is easy to manipulate, which can provide more information for the quality control of Forsythia suspensa dispensing granule.
