CAJ | 학술논문

为探讨在温室中进行栒子微枝试管内生根的方法和效果，将栒子试管苗茎段接种于灭菌的土壤支撑培养基中，并将培养瓶放入日光温室内，分别在不同遮荫条件下和不同培养时期内进行生根培养，并对温室中试管内生根的栒子试管苗进行气孔开闭状况观察和移栽试验。结果表明，在采用双层遮阳网遮荫的条件下，4月12日至5月22日时期内培养的试管苗生根率达到87.5%，9月2日至10月12日时期内培养的试管苗生根率达到98.1%；温室中培养的栒子试管苗的叶片气孔在黑暗中的关闭率为32.5%，显著高于恒温培养室培养的试管苗（5.0%），表明温室中培养的栒子试管苗在生根过程中已经获得了一定的驯化锻炼；将温室中培养的未经开瓶炼苗的栒子试管苗带坨移入营养钵，在移栽后不覆膜、不喷雾，午后空气相对湿度低至约50%的条件下，移栽成活率达到90%，显著高于恒温培养室培养的试管苗（30.0%）。本结果可进一步降低栒子试管苗的培养成本，并促进栒子组培快繁技术在生产中的应用。
위탐토재온실중진행순자미지시관내생근적방법화효과，장순자시관묘경단접충우멸균적토양지탱배양기중，병장배양병방입일광온실내，분별재불동차음조건하화불동배양시기내진행생근배양，병대온실중시관내생근적순자시관묘진행기공개폐상황관찰화이재시험。결과표명，재채용쌍층차양망차음적조건하，4월12일지5월22일시기내배양적시관묘생근솔체도87.5%，9월2일지10월12일시기내배양적시관묘생근솔체도98.1%；온실중배양적순자시관묘적협편기공재흑암중적관폐솔위32.5%，현저고우항온배양실배양적시관묘（5.0%），표명온실중배양적순자시관묘재생근과정중이경획득료일정적순화단련；장온실중배양적미경개병련묘적순자시관묘대타이입영양발，재이재후불복막、불분무，오후공기상대습도저지약50%적조건하，이재성활솔체도90%，현저고우항온배양실배양적시관묘（30.0%）。본결과가진일보강저순자시관묘적배양성본，병촉진순자조배쾌번기술재생산중적응용。
s: In order to study the method and effect of Cotoneaster hjelmqvistii micro-shoots rooting in vitro, the stem sections of Cotoneaster hjelmqvistii plantlet in vitro were inoculated in the sterilized medium supported by soil and the cultural bottles were located in green-house, then the rooting culture was completed under different shading conditions or during different culture periods respectively. The opening and closing of stoma was observed and the transplanting experiment was done for the Cotoneaster hjelmqvistii plantlet rooted in vitro in green-house. The results show that shaded by two-story shade net, 87.5% of the stem sections rooted when cultured from April 12 to May 22,and 98.1% of the stem sections rooted when cultured from September 2 to October 12. Under dark conditions, the stoma closing rate of the Cotoneaster hjelmqvistii plantlet cultured in green-house was 32.5%, which was obviously higher than that of the plantlet cultured in the constant temperature room(5.0%), and this indicated that the plantlet cultured in green-house had been acclimated during the stage of rooting. Without acclimation in opened bottles, the Cotoneaster hjelmqvistii plantlet cultured in green-house were transplanted with lump into the soil, under the conditions without plastic film covering and spraying, and when the relative air humidity was about 50% after noon, the survival rate of the plantlet was 90.0%, obviously higher than that of the plantlet cultured in the constant temperature room (30.3%). These results can obviously reduce the culture cost of the Cotoneaster hjelmqvistii plantlet in vitro and promote the micro-propagation technology application of Cotoneaster hjelmqvistii to the production.