医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2015年
7期
934-937
,共4页
黄药子%黄独乙素%含量测定%色谱法,高效液相
黃藥子%黃獨乙素%含量測定%色譜法,高效液相
황약자%황독을소%함량측정%색보법,고효액상
Dioscorea bulbifera L.%Diosbulbin B%Content determination%Chromatography,high performance liquid
目的：建立高效液相色谱( HPLC)法测定不同产地黄药子中黄独乙素的含量并比较其差异。方法药材粉末用纯净水直接煎二次,合并水煎液,采用Kromasil-C18(4.6 mm×250 mm,5μm )色谱柱,以乙腈水醋酸=34660.1为流动相,流速为1.0 mL·min-1,检测波长210 nm,柱温35℃。结果黄独乙素在26.0~130.0μg·mL-1范围内线性关系良好(r=0.9999),不同产地黄药子中黄独乙素平均含量：河北>广西>江苏>四川>浙江。结论该方法操作简便、快捷,结果准确、可靠,适用于不同产地黄药子中黄独乙素的含量测定。
目的：建立高效液相色譜( HPLC)法測定不同產地黃藥子中黃獨乙素的含量併比較其差異。方法藥材粉末用純淨水直接煎二次,閤併水煎液,採用Kromasil-C18(4.6 mm×250 mm,5μm )色譜柱,以乙腈水醋痠=34660.1為流動相,流速為1.0 mL·min-1,檢測波長210 nm,柱溫35℃。結果黃獨乙素在26.0~130.0μg·mL-1範圍內線性關繫良好(r=0.9999),不同產地黃藥子中黃獨乙素平均含量：河北>廣西>江囌>四川>浙江。結論該方法操作簡便、快捷,結果準確、可靠,適用于不同產地黃藥子中黃獨乙素的含量測定。
목적：건립고효액상색보( HPLC)법측정불동산지황약자중황독을소적함량병비교기차이。방법약재분말용순정수직접전이차,합병수전액,채용Kromasil-C18(4.6 mm×250 mm,5μm )색보주,이을정수작산=34660.1위류동상,류속위1.0 mL·min-1,검측파장210 nm,주온35℃。결과황독을소재26.0~130.0μg·mL-1범위내선성관계량호(r=0.9999),불동산지황약자중황독을소평균함량：하북>엄서>강소>사천>절강。결론해방법조작간편、쾌첩,결과준학、가고,괄용우불동산지황약자중황독을소적함량측정。
Objective To establish a HPLC method for determining and comparing the contents of diosbulbin B in <br> Dioscorea bulbifera L. from different regions. Methods The medicine powders were extracted twice by water, then the water extracts were combined and detected by HPLC at 35℃,with Kromasil-C18 column (4. 6 mm×250 mm,5μm) and a mobile phase of acetonitrile-water-glacial-acetic acid (34660. 1). The flow rate was 1. 0 mL·min-1 and the detection wavelength was 210 nm. Results The linear range of diosbulbin B was 26. 0-260. 0 μg·mL-1(r=0. 999 9). The average content of diosbulbin B in Dioscorea bulbifera L. from different areas was that of Hebei>Guangxi>Jiangsu>Sichuan>Zhejiang. Conclusion The method is simple,quick,accurate and suitable for the determination of diosbulbin B in Dioscorea bulbifera L. from different regions.
