CAJ | 학술논문

目的：探讨非诺贝特对酿酒酵母基因表达谱的影响，在基因表达水平上分析其作用机理。方法：采用非诺贝特（100μg/mL）处理酿酒酵母90min后，抽提细胞RNA进行反转录荧光标记cRNA，与基因表达谱芯片进行杂交，用激光扫描仪检测杂交信号，并用相应软件进行分析。结果：与正常对照组相比，非诺贝特导致19个已知的基因表达明显上升（诱导倍数>1.5），同时导致14个已知的基因表达明显下降（抑制倍数>1.5）；另外，非诺贝特显著诱导了α-亚麻酸、脂肪酸代谢和降解信号通路，抑制了丙酮酸和碳代谢、糖酵解和糖异生、二羧酸代谢等信号通路（P<0.05）。结论：非诺贝特可以明显调节脂肪酸、糖和尿酸代谢信号通路。
목적：탐토비낙패특대양주효모기인표체보적영향，재기인표체수평상분석기작용궤리。방법：채용비낙패특（100μg/mL）처리양주효모90min후，추제세포RNA진행반전록형광표기cRNA，여기인표체보심편진행잡교，용격광소묘의검측잡교신호，병용상응연건진행분석。결과：여정상대조조상비，비낙패특도치19개이지적기인표체명현상승（유도배수>1.5），동시도치14개이지적기인표체명현하강（억제배수>1.5）；령외，비낙패특현저유도료α-아마산、지방산대사화강해신호통로，억제료병동산화탄대사、당효해화당이생、이최산대사등신호통로（P<0.05）。결론：비낙패특가이명현조절지방산、당화뇨산대사신호통로。
Objective:To investigate the effect of fenofibrate on saccharomyces cerevisiae gene expression profile and study the mechanism at gene expression level. Methods:After Saccharomyces cerevisiae was treated with fenofibrate (100μg/mL) for 90min, cell RNA was extracted and re-verse-transcripted into fluorescently-labeled cRNA which was hybridized with gene expression profile chip. Furthermore, laser scanner was used to detect the hybridization signal. Finally, data was analyzed by related software. Results:Compared with normal control group, fenofibrate obviously increased expression of 19 known genes (fold>1.5), and markedly reduced expression of 14 known genes (fold>1.5);in addition, fenofibrate signifi-cantly elevated the alpha-Linolenic acid metabolism, fatty acid degradation and metabolism signal pathway and decreased pyruvate metabolism, gly-colysis/gluconeogenesis and glyoxylate and dicarboxylate metabolism signal pathway (P<0.05). Conclusion:The results suggest that the fatty acid, glycogen and uric acid metabolism signal pathways could be significantly regulated by fenofibrate.