沈阳化工大学学报
瀋暘化工大學學報
침양화공대학학보
JOURNAL OF SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
2015年
2期
118-122
,共5页
蘑菇培养基质%几丁质酶%壳聚糖酶%酶活力测定%抑菌活性
蘑菇培養基質%幾丁質酶%殼聚糖酶%酶活力測定%抑菌活性
마고배양기질%궤정질매%각취당매%매활력측정%억균활성
mushroom waste medium%chitinase%chitosanase%determination of enzyme activity%antimicrobial activity
该研究分别从9种不同品种的蘑菇培养基质中提取几丁质酶和壳聚糖酶2种胞外酶并测定其酶活力.结果表明：平菇的培养基质中几丁质酶的活性最高，达33 U/g培养基质；金针菇的培养基质中壳聚糖酶的活性最高，达21．062 U/g培养基质.根据酶活测定的结果选取其中3种酶活较高的培养基质进行抑菌活性的研究.结果表明：3种培养基质的粗酶液对蜡样芽孢杆菌、番茄细菌性溃疡病菌和巨大芽孢杆菌3种病原菌均有抑制活性，且对巨大芽孢杆菌的抑菌活性最强.
該研究分彆從9種不同品種的蘑菇培養基質中提取幾丁質酶和殼聚糖酶2種胞外酶併測定其酶活力.結果錶明：平菇的培養基質中幾丁質酶的活性最高，達33 U/g培養基質；金針菇的培養基質中殼聚糖酶的活性最高，達21．062 U/g培養基質.根據酶活測定的結果選取其中3種酶活較高的培養基質進行抑菌活性的研究.結果錶明：3種培養基質的粗酶液對蠟樣芽孢桿菌、番茄細菌性潰瘍病菌和巨大芽孢桿菌3種病原菌均有抑製活性，且對巨大芽孢桿菌的抑菌活性最彊.
해연구분별종9충불동품충적마고배양기질중제취궤정질매화각취당매2충포외매병측정기매활력.결과표명：평고적배양기질중궤정질매적활성최고，체33 U/g배양기질；금침고적배양기질중각취당매적활성최고，체21．062 U/g배양기질.근거매활측정적결과선취기중3충매활교고적배양기질진행억균활성적연구.결과표명：3충배양기질적조매액대사양아포간균、번가세균성궤양병균화거대아포간균3충병원균균유억제활성，차대거대아포간균적억균활성최강.
This experiment extracted chitinase and chitosanase from 9 species of mushroom waste media and then determined their enzyme activity. The research showed that the chitinase activity of waste medi-um of Pleurotus ostreatus could reach to 33 U/g waste medium,which was the best;the chitosanase activi-ty of waste medium of Flammulina velutipes could reach to 21. 062 U/g waste medium,which was the best. According to the result,3 species of mushroom waste media whose chitinase and chitosanase activity were higher were screened to assay their antimicrobial activity. The research showed that this 3 species of crude extract of chitinase and chitosanase from waste medium all had inhibitory activity against Bacillus cereus,Clavibacter michiganensis subsp. michiganensis and Bacillus megaterium,which was the best.
