CAJ | 학술논문

目的：探讨蛇床子素( Ost)对神经干细胞( NSCs)分化的影响及机制。方法体外分离并培养新生小鼠脑源NSCs,免疫细胞化学法鉴定；取第5代NSCs置于含不同浓度(0,10,50,100μmol·L-1)Ost的分化培养液中,免疫荧光细胞化学法检测NSCs分化为神经元、星形胶质细胞和少突胶质细胞情况；RT-PCR检测Notch 1基因及其靶基因Mash 1和Neurogenin 2(Ngn2)表达情况。结果免疫荧光细胞化学法显示神经球显 Nestin/Sox2阳性,即所培养的细胞为NSCs；Ost可促进NSCs更多地向神经元(P<0.01)和少突胶质细胞(P<0.05)分化,而非星形胶质细胞。 Ost可减少Notch 1(P<0.01)并增加Ngn 2(P<0.01)mRNA表达,其中以Ost 100μmol·L-1组最明显。结论 Ost可促使NSCs更多地向神经元和少突胶质细胞分化,其机制可能与Ost抑制Notch信号通路有关。
목적：탐토사상자소( Ost)대신경간세포( NSCs)분화적영향급궤제。방법체외분리병배양신생소서뇌원NSCs,면역세포화학법감정；취제5대NSCs치우함불동농도(0,10,50,100μmol·L-1)Ost적분화배양액중,면역형광세포화학법검측NSCs분화위신경원、성형효질세포화소돌효질세포정황；RT-PCR검측Notch 1기인급기파기인Mash 1화Neurogenin 2(Ngn2)표체정황。결과면역형광세포화학법현시신경구현 Nestin/Sox2양성,즉소배양적세포위NSCs；Ost가촉진NSCs경다지향신경원(P<0.01)화소돌효질세포(P<0.05)분화,이비성형효질세포。 Ost가감소Notch 1(P<0.01)병증가Ngn 2(P<0.01)mRNA표체,기중이Ost 100μmol·L-1조최명현。결론 Ost가촉사NSCs경다지향신경원화소돌효질세포분화,기궤제가능여Ost억제Notch신호통로유관。
Objective To investigate the effects of osthole on neural stem cells ( NSCs) differentiation and explore the potential mechanism. Methods Brain-derived NSCs from newborn mice were isolated and cultured in vitro and determined by immunofluorescence. The P5 generations of NSCs were placed in culture solution with osthole at concentrations of (0,10,50, 100 μmol·L-1 ) . The neuron, astrocyte and oligodendroglia cell differentiation were determined by immunofluorescence. The mRNA expression of Notch 1 and its target genes Mash 1 and Neurogenin 2 were assessed by RT-PCR. Results The neurosphere displayed Nestin and Sox 2-postive by immunofluorescence, suggesting that the cultured cells were NSCs. Osthole promoted NSCs differentiating into more neuron(P<0. 01) and oligodendrocyte(P<0. 05), but not astrocyte. Meanwhile, osthole significantly reduced the mRNA expression of Notch 1(P<0. 01) and increased Ngn 2(P<0. 01)at the dose of 100 μmol·L-1. Conclusion Osthole enhances NSCs differentiating into more neuron and oligodendrocyte via probablly inhibiting Notch signal pathway.