CAJ | 학술논문

目的：探讨阿魏酸钠（sodium ferulate, SF）对溃疡性结肠炎治疗的可能相关机制。方法：小鼠随机分4成组空白对照（NC）组、模型（MD）组、低剂量（SFLD）、高剂量（SFHD）阿魏酸钠治疗组，NC组采用正常饮用水饲养，采用5%葡聚糖硫酸钠（Dextran sulfate Sodium, DSS）制作UC小鼠模型，造模7 d成模后分为MD组、SFLD组和SFHD组，予药物干预治疗后续7 d。记录分析疾病活动指数（DAI）及脾脏指数（Spleen Index SI），取结肠组织行HE染色；Western blot检测结肠NF-KB表达水平；ELISA法测小鼠肠粘膜NF-kB活性。结果：SFLD组、SFHD组小鼠第8～14 d DAI明显低于MD组（P<0.05）；SFLD组、SFHD组小鼠第14 d SI明显低于MD组（P<0.05）；MD组SI明显高于NC组（P<0.05）；SFLD组、SFHD组结肠NF-kB表达和活性明显低于MD组；MD组结肠NF-kB表达和活性明显高于NC组（P<0.05）；MD组NF-kB表达和活性明显高于NC组（P<0.05）。结论：阿魏酸钠可以通过降低结肠NF-kB表达及活性；抑制脾脏代偿性增大，从而减轻肠道的炎症反应及抑制脾脏的免疫反应，改善UC全身炎症状态及免疫应答，对UC发挥治疗作用。
목적：탐토아위산납（sodium ferulate, SF）대궤양성결장염치료적가능상관궤제。방법：소서수궤분4성조공백대조（NC）조、모형（MD）조、저제량（SFLD）、고제량（SFHD）아위산납치료조，NC조채용정상음용수사양，채용5%포취당류산납（Dextran sulfate Sodium, DSS）제작UC소서모형，조모7 d성모후분위MD조、SFLD조화SFHD조，여약물간예치료후속7 d。기록분석질병활동지수（DAI）급비장지수（Spleen Index SI），취결장조직행HE염색；Western blot검측결장NF-KB표체수평；ELISA법측소서장점막NF-kB활성。결과：SFLD조、SFHD조소서제8～14 d DAI명현저우MD조（P<0.05）；SFLD조、SFHD조소서제14 d SI명현저우MD조（P<0.05）；MD조SI명현고우NC조（P<0.05）；SFLD조、SFHD조결장NF-kB표체화활성명현저우MD조；MD조결장NF-kB표체화활성명현고우NC조（P<0.05）；MD조NF-kB표체화활성명현고우NC조（P<0.05）。결론：아위산납가이통과강저결장NF-kB표체급활성；억제비장대상성증대，종이감경장도적염증반응급억제비장적면역반응，개선UC전신염증상태급면역응답，대UC발휘치료작용。
Objective:To investigate the mechanismof sodiumin the treatmentof ulcerative colitis. [Method] Mice were randomly divided i nto 4 groups:bl ank c ontrol (N C) , model group (MDgroup), lowdo se SF group(SFLD), high dos e SF group (SFHD). N C group with normal drinkingwater feeding, After 7 days of using5%dextran sulfate sodiumto make UC mouse model, the models with 7 days of drugi ntervention were divided into MDgroup, SFLD group and SFHD group. Re cord and a nalyze the di sease a ctivity index (DAI) and spleen index (Spleen Index SI), colon tissue were stained with HE;NF-KB expression of colon were detected by western blot;NF-kB a ctivity of intestinal mucosal were measured by E LISA. R esult:AmongSFLD group and SFHD group of mice on d ays 8-14, DAI was s ignificantly l ower than that of MDgroup ( P<0.05); AmongSFLD group and SFHD group of mice on 14 da ys, SI was significantly lower than thatof MD group(P<0.05);the SI of MD group was significantly higher than thatof group NC (P<0.05);AmongSFLD group and SFHD group, the expression and activity of NF-kB in colon was significantly lower than thatof MD group;the active of NF-kB expression in MD group was significantly higher than thatin NC group (P<0.05);the activity of NF-kB expression in MD group was higher than thatin NC group (P<0.05). Conclusion:Sodiumferulate can reduce the expression and activity of NF-kB of colon, inhibitthe s pleen’s c ompensatory increase, r elieve the i nflammatory r eaction of intestine,alleviate i mmune r eaction of s pleen,improve sy stemic inflammatory state and immune response of UC and play a role in the treatmentof UC.