CAJ | 학술논문

目的了解深圳地区急性呼吸道感染患者中15种常见呼吸道病毒及其亚型的感染状况.方法于2012年10月-2013年9月在深圳市两家流感监测哨点医院采集了1603例流感样病例的咽拭子样本,应用荧光定量PCR方法检测15种常见呼吸道病毒及其亚型,分析病例流行病学特征.结果 1603份标本中共检出呼吸道病毒阳性801份,阳性率为49.97％.鼻病毒的检出率最高,为15.53％(249/1603),其它检出率较高的病毒为甲型流感病毒(12.10％,194/1603)、呼吸道合胞病毒B型(6.8％,109/1603)、腺病毒(5.24％,84/1603)、副流感病毒3型(2.87％,46/1603)、间质肺病毒B型(2.37％,38/1603)、乙型流感(1.37％,22/1603)和冠状病毒OC43型(1.06％,17/1603).呼吸道病毒总阳性率3月份最高,11月份最低.各种病毒的流行季节不同,鼻病毒夏季高发,呼吸道合胞病毒在冬春季和夏季流行,腺病毒在冬季和夏季高发.0-4岁和5-14岁组儿童的呼吸道病毒阳性率(分别为60.4％和49.3％)高于其它年龄组.结论深圳地区存在多种呼吸道病毒流行,鼻病毒、甲型流感病毒、呼吸道合胞病毒和腺病毒为主要病原体,其流行季节各不相同,低年龄组儿童为呼吸道病毒感染的高危人群.
목적 료해심수지구급성호흡도감염환자중15충상견호흡도병독급기아형적감염상황.방법 우2012년10월-2013년9월재심수시량가류감감측초점의원채집료1603례류감양병례적인식자양본,응용형광정량PCR방법검측15충상견호흡도병독급기아형,분석병례류행병학특정.결과 1603빈표본중공검출호흡도병독양성801빈,양성솔위49.97％.비병독적검출솔최고,위15.53％(249/1603),기타검출솔교고적병독위갑형류감병독(12.10％,194/1603)、호흡도합포병독B형(6.8％,109/1603)、선병독(5.24％,84/1603)、부류감병독3형(2.87％,46/1603)、간질폐병독B형(2.37％,38/1603)、을형류감(1.37％,22/1603)화관상병독OC43형(1.06％,17/1603).호흡도병독총양성솔3월빈최고,11월빈최저.각충병독적류행계절불동,비병독하계고발,호흡도합포병독재동춘계화하계류행,선병독재동계화하계고발.0-4세화5-14세조인동적호흡도병독양성솔(분별위60.4％화49.3％)고우기타년령조.결론 심수지구존재다충호흡도병독류행,비병독、갑형류감병독、호흡도합포병독화선병독위주요병원체,기류행계절각불상동,저년령조인동위호흡도병독감염적고위인군.
Objective To determine the viral pathogens of influenza-like illness (ILI) and epidemiological characteristics of 15 respiratory viruses and its subtypes in Shenzhen.Methods From Oct.2012 to Sep.2013,a total of 1603 cases of ILI were sampled and detected for 15 respiratory viruses and their subtypes (influenza viruses type A and B,human rhinovirus (HRV),respiratory syncytial virus (RSV,type A and B),adenovirus (AdV),parainfluenza viruses (PIV,type Ⅰ,Ⅱ and Ⅲ),human coronaviruses (hCoV,type 229E,OC43,NL63,and HKU1),human metapneumovirus (hMPV,type A and B) using real-time reverse transcription polymerase chain reaction (RT-PCR).Results A total of 801 cases of ILI were tested positive for respiratory viruses with positive rate of 49.97％ (801/1603).HRV had the highest positive rate (15.53％,249/1603),followed by influenza virus (type A,12.10％,194/1603),RSV (type B,6.8％,109/1603),AdV (5.24％,84/1603),PIV (type Ⅲ,2.87％,46/1603),hMPV (type B,2.37％,3 8/1603),influenza (type B,1.37％,22/1603),and hCoV (type OC43,1.06％,17/1603).The highest detection rate of respiratory viruses were observed in March,with the lowest in November.HRV infection was prevalent primarily in summer while RSV infection was common in spring and summer.AdV infection peaked in winter and summer.Children in age groups 0-4 and 5-14 had significantly higher infection rate of respiratory viruses (60.4％and 49.3％,respectively) than other age groups.Concluusions HRV,influenza virus,RSV and AdV accounted for majority of viral etiology spectrum of ILI with different epidemic season,children under age of 15 were high risk population for infection of respiratory viruses.