中国继续医学教育
中國繼續醫學教育
중국계속의학교육
CHINA CONTINUING MEDICAL EDUCATION
2015年
16期
47-48
,共2页
bFGF基因%骨髓间充质细胞%细胞转染
bFGF基因%骨髓間充質細胞%細胞轉染
bFGF기인%골수간충질세포%세포전염
BFGF gene%Bone marrow mesenchymal cells%Cell transfection
目的：探讨bFGF（外源性碱性成纤维细胞生长因子）基因转染骨髓间充质细胞（MSC）的鉴定方法。方法提取大鼠骨髓间充质细胞，体外培养后，随机分为bFGF组（构建bFGF基因病毒质粒，移植到MSC），正常组和阴性组（转染Ad.GFP），通过免疫組化染色法观察MSC的形态，同时应用Western-Blot法分析bFGF基因表达情况。结果阴性组和正常组均无转染bFGF，bFGF组胞浆呈黄色，Western-Blot分析可见bFGF特异性条带，其表达量为0.85，高于正常组及阴性组， P＞0.05，差异不具有统计学意义。结论将病毒作为载体能够成功实现bFGF基因转染MSC，得到目的基因阳性表达。
目的：探討bFGF（外源性堿性成纖維細胞生長因子）基因轉染骨髓間充質細胞（MSC）的鑒定方法。方法提取大鼠骨髓間充質細胞，體外培養後，隨機分為bFGF組（構建bFGF基因病毒質粒，移植到MSC），正常組和陰性組（轉染Ad.GFP），通過免疫組化染色法觀察MSC的形態，同時應用Western-Blot法分析bFGF基因錶達情況。結果陰性組和正常組均無轉染bFGF，bFGF組胞漿呈黃色，Western-Blot分析可見bFGF特異性條帶，其錶達量為0.85，高于正常組及陰性組， P＞0.05，差異不具有統計學意義。結論將病毒作為載體能夠成功實現bFGF基因轉染MSC，得到目的基因暘性錶達。
목적：탐토bFGF（외원성감성성섬유세포생장인자）기인전염골수간충질세포（MSC）적감정방법。방법제취대서골수간충질세포，체외배양후，수궤분위bFGF조（구건bFGF기인병독질립，이식도MSC），정상조화음성조（전염Ad.GFP），통과면역조화염색법관찰MSC적형태，동시응용Western-Blot법분석bFGF기인표체정황。결과음성조화정상조균무전염bFGF，bFGF조포장정황색，Western-Blot분석가견bFGF특이성조대，기표체량위0.85，고우정상조급음성조， P＞0.05，차이불구유통계학의의。결론장병독작위재체능구성공실현bFGF기인전염MSC，득도목적기인양성표체。
Objective To explore the identification method of basic fibroblast growth factor (bFGF) exogenous transfection to MSC (bone marrow cells). Methods The bone marrow mesenchymal cell of rats were extracted in vitro culture, divided into bFGF group (build bFGF gene plasmid and transplanted to MSC), normal group and negative group (transfection Ad.GFP) randomly. The MSC form was observed by immunohistochemical staining method, at the same time, analyzed bFGF gene expression by Western-Blot method. Results There were no transfected bFGF in negative group and normal group, bFGF cytoplasm was yellow, Western-Blot analysis showed the bFGF specific band, its expression quantity was 0.85, it was higher than t the normal control group and negative control group, P>0.05 ,had no difference statistically significance. Conclusions The bFGF transfects to MSC gene can be successful by use virus as a vector, the positive expression can be obtained.
