中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2015年
3期
300-302
,共3页
麻醉药,吸入%麻醉%婴儿%认知障碍%GAP-43蛋白%神经细胞黏附分子类%海马%神经元
痳醉藥,吸入%痳醉%嬰兒%認知障礙%GAP-43蛋白%神經細胞黏附分子類%海馬%神經元
마취약,흡입%마취%영인%인지장애%GAP-43단백%신경세포점부분자류%해마%신경원
Anesthetics,inhalation%Anesthesia%Infant%Cognition disorders%GAP-43 protein%Neural cell adhesion molecules%Hippocampus%Neurons
目的 评价七氟醚麻醉对幼鼠海马神经元生长相关蛋白-43(GAP-43)和神经细胞黏附分子(NCAM)表达的影响.方法 清洁级健康SD大鼠36只,雌雄不拘,7日龄,体重15 ～ 20 g,采用随机数字表法分为4组(n=9):L组吸入1.5％七氟醚6h;H1组和H2组分别吸入3％七氟醚2和6h;C组吸入30％氧气.待大鼠生长至出生后14d时开始Morris水迷宫训练7d,进行定位航行实验和空间探索实验.水迷宫测试结束后断头处死大鼠取海马,采用Western blot法检测海马神经元GAP-43和NCAM的表达.结果 与C组比较,L组、H1组和H2组逃避潜伏期延长,平台象限停留时间缩短,海马神经元GAP-43表达下调,L组和H2组穿越平台次数减少(P＜0.05).各组海马神经元NCAM表达比较差异无统计学意义(P＞0.05).结论 七氟醚麻醉降低幼鼠认知功能的机制可能与其下调海马神经元GAP-43表达有关,与NCAM表达无关.
目的 評價七氟醚痳醉對幼鼠海馬神經元生長相關蛋白-43(GAP-43)和神經細胞黏附分子(NCAM)錶達的影響.方法 清潔級健康SD大鼠36隻,雌雄不拘,7日齡,體重15 ～ 20 g,採用隨機數字錶法分為4組(n=9):L組吸入1.5％七氟醚6h;H1組和H2組分彆吸入3％七氟醚2和6h;C組吸入30％氧氣.待大鼠生長至齣生後14d時開始Morris水迷宮訓練7d,進行定位航行實驗和空間探索實驗.水迷宮測試結束後斷頭處死大鼠取海馬,採用Western blot法檢測海馬神經元GAP-43和NCAM的錶達.結果 與C組比較,L組、H1組和H2組逃避潛伏期延長,平檯象限停留時間縮短,海馬神經元GAP-43錶達下調,L組和H2組穿越平檯次數減少(P＜0.05).各組海馬神經元NCAM錶達比較差異無統計學意義(P＞0.05).結論 七氟醚痳醉降低幼鼠認知功能的機製可能與其下調海馬神經元GAP-43錶達有關,與NCAM錶達無關.
목적 평개칠불미마취대유서해마신경원생장상관단백-43(GAP-43)화신경세포점부분자(NCAM)표체적영향.방법 청길급건강SD대서36지,자웅불구,7일령,체중15 ～ 20 g,채용수궤수자표법분위4조(n=9):L조흡입1.5％칠불미6h;H1조화H2조분별흡입3％칠불미2화6h;C조흡입30％양기.대대서생장지출생후14d시개시Morris수미궁훈련7d,진행정위항행실험화공간탐색실험.수미궁측시결속후단두처사대서취해마,채용Western blot법검측해마신경원GAP-43화NCAM적표체.결과 여C조비교,L조、H1조화H2조도피잠복기연장,평태상한정류시간축단,해마신경원GAP-43표체하조,L조화H2조천월평태차수감소(P＜0.05).각조해마신경원NCAM표체비교차이무통계학의의(P＞0.05).결론 칠불미마취강저유서인지공능적궤제가능여기하조해마신경원GAP-43표체유관,여NCAM표체무관.
Objective To evaluate the influence of sevoflurane anesthesia on the expression of growth-associated protein 43 (GAP-43) and neural cell adhesion molecule (NCAM) in hippocampal neurons of neonatal rats.Methods Thirty-six pathogen-free Sprague-Dawley rats,aged 7 weeks,weighing 15-20 g,were randomly divided into 4 groups (n =9 each) using a random number table:control group (C group),1.5％ sevoflurane 6 h group (L group),3％ sevoflurane 2 h group (H1 group) and 3％ sevoflurane 6 h group (H2 group).Group L inhaled 1.5％ sevoflurane in oxygen for 6 h.H1 and H2 groups inhaled 3％ sevoflurane in oxygen for 2 and 6 h,respectively.Group C inhaled 30％ oxygcn only.When the neonatal rats were 14 days old,the rats underwent Morris water maze test for 7 consecutive days.Place navigation and spatial probe tests were carried out.After the end of Morris water maze test,the rats were sacrificed,and the hippocampus was obtained for determination of the expression of GAP-43 and NCAM in hippocampal neurons.Results Compared with group C,the escape latency was significantly prolonged,the time of staying at the original platform quadrant was shortened,and the expression of GAP-43 was down-regulated in L,H1 and H2 groups,and the frequency of crossing the original platform was decreased in L and H2 groups.There was no significant difference in NCAM expression among the four groups.Conclusion The mechanism by which sevoflurane anesthesia decreases the cognitive function may be related to down-regulated expression of GAP-43,but not related to NCAM expression in hippocampal neurons of neonatal rats.
