医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2015年
7期
847-850
,共4页
菝葜%活性部位%前列腺增生,良性
菝葜%活性部位%前列腺增生,良性
발계%활성부위%전렬선증생,량성
Smilax china L.%Bioactive fraction%Prostatic hyperplasia,benign
目的：研究菝葜提取物对良性前列腺增生( BPH)的抑制作用,并筛选其活性部位。方法将雄性大鼠随机分为8组,每组6只,分别为假手术组、模型对照组、石油醚组、乙酸乙酯组、正丁醇组、水部位组、大孔树脂部位组、总提取物组。除假手术组外,其他各组均采用丙酸睾酮诱导去势大鼠BPH模型,并分别灌胃给予菝葜各提取部位,连续3周。末次给药后分离血清,检测前列腺酸性磷酸酶( PACP)含量,称取前列腺质量并进行病理学检查,评价菝葜各提取部位抑制BPH活性。结果菝葜各提取部位对大鼠BPH均有一定抑制作用,其中正丁醇部位、水部位和大孔树脂部位能显著抑制大鼠前列腺增生。与模型对照组比较,正丁醇组、水部位组及大孔树脂部位组前列腺指数分别降低了52.80%,50.93%及67.70%,血清PACP含量均显著降低。病理学检查发现,前列腺组织形态均有明显改善。综合各检测指标,菝葜大孔树脂部位抑制BPH效果最佳。结论菝葜提取物对模型大鼠BPH有一定抑制作用,其中大孔树脂部位效果最好,提示其可能为抑制BPH的活性部位。
目的：研究菝葜提取物對良性前列腺增生( BPH)的抑製作用,併篩選其活性部位。方法將雄性大鼠隨機分為8組,每組6隻,分彆為假手術組、模型對照組、石油醚組、乙痠乙酯組、正丁醇組、水部位組、大孔樹脂部位組、總提取物組。除假手術組外,其他各組均採用丙痠睪酮誘導去勢大鼠BPH模型,併分彆灌胃給予菝葜各提取部位,連續3週。末次給藥後分離血清,檢測前列腺痠性燐痠酶( PACP)含量,稱取前列腺質量併進行病理學檢查,評價菝葜各提取部位抑製BPH活性。結果菝葜各提取部位對大鼠BPH均有一定抑製作用,其中正丁醇部位、水部位和大孔樹脂部位能顯著抑製大鼠前列腺增生。與模型對照組比較,正丁醇組、水部位組及大孔樹脂部位組前列腺指數分彆降低瞭52.80%,50.93%及67.70%,血清PACP含量均顯著降低。病理學檢查髮現,前列腺組織形態均有明顯改善。綜閤各檢測指標,菝葜大孔樹脂部位抑製BPH效果最佳。結論菝葜提取物對模型大鼠BPH有一定抑製作用,其中大孔樹脂部位效果最好,提示其可能為抑製BPH的活性部位。
목적：연구발계제취물대량성전렬선증생( BPH)적억제작용,병사선기활성부위。방법장웅성대서수궤분위8조,매조6지,분별위가수술조、모형대조조、석유미조、을산을지조、정정순조、수부위조、대공수지부위조、총제취물조。제가수술조외,기타각조균채용병산고동유도거세대서BPH모형,병분별관위급여발계각제취부위,련속3주。말차급약후분리혈청,검측전렬선산성린산매( PACP)함량,칭취전렬선질량병진행병이학검사,평개발계각제취부위억제BPH활성。결과발계각제취부위대대서BPH균유일정억제작용,기중정정순부위、수부위화대공수지부위능현저억제대서전렬선증생。여모형대조조비교,정정순조、수부위조급대공수지부위조전렬선지수분별강저료52.80%,50.93%급67.70%,혈청PACP함량균현저강저。병이학검사발현,전렬선조직형태균유명현개선。종합각검측지표,발계대공수지부위억제BPH효과최가。결론발계제취물대모형대서BPH유일정억제작용,기중대공수지부위효과최호,제시기가능위억제BPH적활성부위。
Objective To study the effect of extracts of Smilax china L. on inhibition the experimentally induced benign prostatic hyperplasia ( BPH) , and screen the effective fraction. Methods The BPH model was built on the castrated rats by subcutaneous injection of testosterone propionate. Male rats were randomly divided into eight groups ( n=6 ):sham operation, model control, petroleum ether fraction, acetic ether fraction, n-butyl alcohol fraction, water fraction, macroporous resin fraction ( FMR) , and total extracts group. The rats were treated with testosterone propionate by subcutaneous injection for consecutive 3 weeks. Meanwhile, rats were orally administrated with the six extract fractions of S. china L. After the last administration, serum was separated for the determination of prostatic acid phosphatase ( PACP ) , prostate was weighed and histopathological examination was carried out to evaluate the inhibitory effect of S. china L. against BPH. Results All of the six fractions from S. china L. could inhibit BPH, and the n-butanol fraction, water fraction and FMR showed better inhibitory effect, which significantly decreased the prostatic index by 52. 80%, 50. 93% and 67. 70%, respectively, remarkably reduced serum PACP, and notably improved the prostate gland morphology compared with the model group. Among the three fractions, FMR showed the strongest effect against BPH. Conclusion S. china L. ameliorates the experimentally prostatic hyperplasia, and FMR showes the best effect, which might be the bioactive components against BPH.
