CAJ | 학술논문

目的：探讨EBER显色原位杂交技术(CISH)在口腔颌面部淋巴上皮癌中特有的操作与改进方法。方法应用CISH技术，荧光素标记的寡核苷酸探针EBER原位杂交检测试剂盒对40例疑为口腔颌面部淋巴上皮癌的病例进行EBER检测。结果EBER阳性率为95%；阳性信号主要定位在肿瘤上皮细胞巢的细胞核上，呈棕黄色，周围淋巴细胞及正常组织未见明显着色；信号定位准确，染色结果清晰易辨，无背景干扰。结论简化操作EBER原位杂交检测试剂盒能够节省时间、增强信号强度和提高工作效率等。
목적：탐토EBER현색원위잡교기술(CISH)재구강합면부림파상피암중특유적조작여개진방법。방법응용CISH기술，형광소표기적과핵감산탐침EBER원위잡교검측시제합대40례의위구강합면부림파상피암적병례진행EBER검측。결과EBER양성솔위95%；양성신호주요정위재종류상피세포소적세포핵상，정종황색，주위림파세포급정상조직미견명현착색；신호정위준학，염색결과청석역변，무배경간우。결론간화조작EBER원위잡교검측시제합능구절성시간、증강신호강도화제고공작효솔등。
Objective To explore the specific procedure and improvement method of EBER chromogenic in situ hy-bridization (CISH) technology in lymphoepithelial carcinoma of oromaxillo-facial region. Methods CISH technology and fluorescein-labeled oligonucleotide probe EBER in situ hybridization assay kit was applied,and 40 suspected patients with lymphoepithelial carcinoma of oromaxillo-facial region were given EBER testing. Results The EBER positive rate was 95%;the positive signal was mainly located at the nucleus of epithelial cell nest of carcinoma and presented as brownish yellow.Peripheral lymphocytes and normal tissues were not seen obvious coloring;the signal location was accu-rate,coloring results were discernible,no background interruption was detected. Conclusion A simple operation of E-BER in situ hybridization assay kit (BioGenex) is able to save time,strengthen signal intensity and enhance work efficiency.