CAJ | 학술논문

目的：检测小儿单纯性骨囊肿和动脉瘤样骨囊肿标本中骨保护素（OPG）、核因子-kB 受体活化因子配体（RANKL）的表达水平，并与正常骨组织作对照，分析两种蛋白在这两种瘤样病变中的表达是否存在差异及意义。方法共收集小儿单纯性骨囊肿标本31例，动脉瘤样骨囊肿标本23例，正常骨组织标本（对照组）14例。采用免疫组化 SABC 方法检测单纯性骨囊肿、动脉瘤样骨囊肿和对照组中 OPG、RANKL 的表达，并分析其差异性。应用 Imagepro-Plus 6．0图像处理软件及数据处理软件SPSS17．0对图像及所得数据进行分析。结果在单纯性骨囊肿及动脉瘤样骨囊肿中，OPG 的表达量均低于正常骨组织，RANKL 的表达量均高于正常骨组织，其中动脉瘤样骨囊肿 RANKL 的表达量较单纯性骨囊肿更高，差异有统计学意义（P ＜0．05）；单纯性骨囊肿的 OPG 稍高于动脉瘤样骨囊肿，差异无统计学意义（P ＞0．05）。RANKL／OPG 比值动脉瘤样骨囊肿＞单纯性骨囊肿＞正常组织，差异有统计学意义（P ＜0．05）。结论RANKL／OPG 失衡可能是小儿单纯性骨囊肿和动脉瘤样骨囊肿出现溶骨性破坏的原因之一，动脉瘤样骨囊肿术后相对更高的复发率可能与 RANKL 的相对高表达有关。
목적：검측소인단순성골낭종화동맥류양골낭종표본중골보호소（OPG）、핵인자-kB 수체활화인자배체（RANKL）적표체수평，병여정상골조직작대조，분석량충단백재저량충류양병변중적표체시부존재차이급의의。방법공수집소인단순성골낭종표본31례，동맥류양골낭종표본23례，정상골조직표본（대조조）14례。채용면역조화 SABC 방법검측단순성골낭종、동맥류양골낭종화대조조중 OPG、RANKL 적표체，병분석기차이성。응용 Imagepro-Plus 6．0도상처리연건급수거처리연건SPSS17．0대도상급소득수거진행분석。결과재단순성골낭종급동맥류양골낭종중，OPG 적표체량균저우정상골조직，RANKL 적표체량균고우정상골조직，기중동맥류양골낭종 RANKL 적표체량교단순성골낭종경고，차이유통계학의의（P ＜0．05）；단순성골낭종적 OPG 초고우동맥류양골낭종，차이무통계학의의（P ＞0．05）。RANKL／OPG 비치동맥류양골낭종＞단순성골낭종＞정상조직，차이유통계학의의（P ＜0．05）。결론RANKL／OPG 실형가능시소인단순성골낭종화동맥류양골낭종출현용골성파배적원인지일，동맥류양골낭종술후상대경고적복발솔가능여 RANKL 적상대고표체유관。
Objetive To explore the expression of osteoprotegerin (OPG)and receptor activator nuclear factor κ-B ligand (RANKL)in pediatric bone tumor-like lesions (bone cysts,aneurysmal bone cysts). Methods 31 specimens of simple bone cysts,23 of aneurysmal bone cysts were collected during surgery and were taken as experimental group.Normal bone tissues from 14 patients were collected as normal controls.The expressions of OPG and RANKL were detected by immunohistochemical staining and quantified by computer image analysis software.Results Compared with normal controls,the expression of RANKL was significantly higher in simple bone cysts and aneurysmal bone cysts,whereas the expression of OPG was significantly lower (P <0.05).The expression of RANKL of aneurysmal bone cysts was significantly higher than that of simple bone cysts (P <0.05).The expression of OPG of simple bone cysts was slightly higher than that of aneurysmal bone cysts,but the difference was not statistically significant (P >0.05).The aneurysmal bone cysts had the highest ratio of RANKL to OPG,followed by simple bone cysts,then the normal controls (P <0.05). Con-clusions Abnormal expression of RANKL and OPG is associated with pediatric bone tumor-like lesions like simple bone cysts and aneurysmal bone cysts.