CAJ | 학술논문

目的：分析标本放置时间及温度对血脂测定结果的影响。方法随机选取2014年3月～2014年6月我院100例参与职工健康体检的志愿者作为测试对象，采集空腹静脉血，将离心后的血浆与血清分装，分别放置于不同温度条件下（22℃，4℃，-20℃），并测定不同保存时间的血清测定结果。结果22℃、4℃高密度脂蛋白胆固醇（HDL-C）放置不同时间测定值，P＞0.05，差异不具有统计学意义；22℃内血清胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）检测结果相对稳定，P＜0.05，差异不具有统计学意义；-20℃保存7天的标本除LDL-C外，HDL-C、TC、TG均与即刻检测结果有差别，P＜0.05，差异具有统计学意义。结论在室温4℃条件下保存的分离标本，2～4 h内血脂检测结果稳定，不会对检测结果产生影响，如要得到较为可靠的检验结果，则血清标本不宜长时间低温放置。
목적：분석표본방치시간급온도대혈지측정결과적영향。방법수궤선취2014년3월～2014년6월아원100례삼여직공건강체검적지원자작위측시대상，채집공복정맥혈，장리심후적혈장여혈청분장，분별방치우불동온도조건하（22℃，4℃，-20℃），병측정불동보존시간적혈청측정결과。결과22℃、4℃고밀도지단백담고순（HDL-C）방치불동시간측정치，P＞0.05，차이불구유통계학의의；22℃내혈청담고순（TC）、삼선감유（TG）、저밀도지단백담고순（LDL-C）검측결과상대은정，P＜0.05，차이불구유통계학의의；-20℃보존7천적표본제LDL-C외，HDL-C、TC、TG균여즉각검측결과유차별，P＜0.05，차이구유통계학의의。결론재실온4℃조건하보존적분리표본，2～4 h내혈지검측결과은정，불회대검측결과산생영향，여요득도교위가고적검험결과，칙혈청표본불의장시간저온방치。
Objective To explore the inlfuence of blood fat test results of specimen storage period and temperature. Methods Randomly selected 100 cases volunteer enjoyed staff physical examination as text subjects, fasting venous blood, plasma and serum after centrifugation, were placed in different temperatures (22℃, 4℃,-20℃) detection results of serum were determined at different storage time. Results 22 ℃ and 4 ℃ high density lipoprotein cholesterol (HDL-C) for different time, P>0.05, had no difference statistically signiifcance, 22 ℃in serum cholesterol (TC), three glycerol (TG), low density lipoprotein cholesterol (LDL-C) detection results are relatively stable, P>0.05, had no difference statistically significance,-20℃preserved specimens except LDL-C 7 days, HDL-C, TC, TG and the immediate detection results are different, P<0.05, had difference statistically signiifcance. Conclusion Isolated specimens at room temperature under 4℃, 2~4 hours in blood fat stability test results, will not affect the test results, such as to get a more reliable test results, the serum specimens should not be placed at low temperature for a long time.