中国美容医学
中國美容醫學
중국미용의학
CHINESE JOURNAL OF AESTHETIC MEDICINE
2015年
11期
42-47
,共6页
朱瑞祥%李加辅%许言文%钱璇%马少林
硃瑞祥%李加輔%許言文%錢璇%馬少林
주서상%리가보%허언문%전선%마소림
异常黑胆质成熟剂%增生性瘢痕%成纤维细胞%TGF-β
異常黑膽質成熟劑%增生性瘢痕%成纖維細胞%TGF-β
이상흑담질성숙제%증생성반흔%성섬유세포%TGF-β
abnormal savda munziq%Hyperplastic scar%fibroblasts%TGF-β1
目的:研究维药异常黑胆质成熟剂(abnormal savda munziq,ASMq)对TGF-β1诱导增生性瘢痕成纤维细胞的影响。方法:组织块法体外培养人增生性瘢痕成纤维细胞,将其分为6组:对照组:加入等体积的培养液;实验组:加或不加不同浓度ASMq(0.2mg/ml,0.4mg/ml,0.6mg/ml,1.0mg/ml)及浓度为5ng/ml的TGF-β1;CCK-8法检测成纤维细胞增殖情况,应用western blot,RT-PCR检测CollagenⅠ及Smad7表达情况。结果:ASMq降低了TGF-β1诱导的成纤维细胞增殖(P<0.05);ASMq下调了TGF-β1诱导的成纤维细胞CollagenⅠmRNA及CollagenⅠ的表达(P<0.05),同时,ASMq明显增加了被TGF-β1抑制的Smad7mRNA和Smad7的表达(P<0.05)。结论:研究结果表明ASMq通过促进Smad7合成来阻断增生性瘢痕成纤维细胞TGF-β/Smad通路信号转导,从而降低成纤维细胞增殖及CollagenⅠ合成。
目的:研究維藥異常黑膽質成熟劑(abnormal savda munziq,ASMq)對TGF-β1誘導增生性瘢痕成纖維細胞的影響。方法:組織塊法體外培養人增生性瘢痕成纖維細胞,將其分為6組:對照組:加入等體積的培養液;實驗組:加或不加不同濃度ASMq(0.2mg/ml,0.4mg/ml,0.6mg/ml,1.0mg/ml)及濃度為5ng/ml的TGF-β1;CCK-8法檢測成纖維細胞增殖情況,應用western blot,RT-PCR檢測CollagenⅠ及Smad7錶達情況。結果:ASMq降低瞭TGF-β1誘導的成纖維細胞增殖(P<0.05);ASMq下調瞭TGF-β1誘導的成纖維細胞CollagenⅠmRNA及CollagenⅠ的錶達(P<0.05),同時,ASMq明顯增加瞭被TGF-β1抑製的Smad7mRNA和Smad7的錶達(P<0.05)。結論:研究結果錶明ASMq通過促進Smad7閤成來阻斷增生性瘢痕成纖維細胞TGF-β/Smad通路信號轉導,從而降低成纖維細胞增殖及CollagenⅠ閤成。
목적:연구유약이상흑담질성숙제(abnormal savda munziq,ASMq)대TGF-β1유도증생성반흔성섬유세포적영향。방법:조직괴법체외배양인증생성반흔성섬유세포,장기분위6조:대조조:가입등체적적배양액;실험조:가혹불가불동농도ASMq(0.2mg/ml,0.4mg/ml,0.6mg/ml,1.0mg/ml)급농도위5ng/ml적TGF-β1;CCK-8법검측성섬유세포증식정황,응용western blot,RT-PCR검측CollagenⅠ급Smad7표체정황。결과:ASMq강저료TGF-β1유도적성섬유세포증식(P<0.05);ASMq하조료TGF-β1유도적성섬유세포CollagenⅠmRNA급CollagenⅠ적표체(P<0.05),동시,ASMq명현증가료피TGF-β1억제적Smad7mRNA화Smad7적표체(P<0.05)。결론:연구결과표명ASMq통과촉진Smad7합성래조단증생성반흔성섬유세포TGF-β/Smad통로신호전도,종이강저성섬유세포증식급CollagenⅠ합성。
Objective To investigate the effect of uighur medicine abnormal savda munziq on the TGF-β1 induced in hypertrophic scar fibroblasts. Methods Using tissue explant cultures of human hypertrophic scar fibroblasts, divided into six group:control group:add an equal volume of culture medium;and experimental group:treated with TGF-β1 with or without different concentration of ASMq (0.2 mg/ml,0.4 mg/ml,0.6 mg/ml,1.0mg/ml).CCK-8 assay was adopted to detect the proliferation of fibroblasts.western blot and RT-PCR were performed to detect the expression of CollagenⅠand Smad7. Results ASMq reduced the TGF-β1 induced in fibroblasts proliferation(P<0.05);ASMq down-regulation the fibroblast expression of CollagenⅠmRNA and CollagenⅠinduced by TGF-β1 (P<0.05),In addition,ASMq significantly increased the expression of Smad7 mRNA and Smad7 which was suppressed by TGF-β1(P<0.05). Conclusions Our results demonstrate that ASMq blocks the TGF-β/Smad signalling pathway in hypertrophic scar fibroblast by up regulation of Smad7,thereby reducing the proliferation of fibroblasts and synthesis of CollagenⅠ.