CAJ | 학술논문

目的：研究三七总皂苷(PNS)对大鼠股骨骨折后肾髓质浅层细胞凋亡的影响及其对骨折后肾损伤的保护作用。方法将102只 Wistar 大鼠随机分为单纯骨折组(36只)、骨折治疗组(36只)、正常对照组(30只)；前两组大鼠分别在骨折建模后1、6、12、24、36、48 h 时各处死6只,在相同时间段正常对照组处死5只。HE 染色观察肾髓质的病理变化,TUNEL 染色观察凋亡细胞,免疫组化及原位杂交技术检测 Bcl-2和 Bax 在肾髓质浅层组织中的表达。结果单纯骨折组中位于肾髓质浅层的肾小管上皮细胞可见轻度颗粒变性,间质小血管轻度扩张淤血；骨折治疗组与单纯骨折组相比,病变程度较轻。在单纯骨折组中,Bcl-2及 Bcl-2 mRNA 表达在1~36 h 明显高于正常对照组(P <0.01)；Bax 表达在12~36 h 较正常对照组高(P <0.01),Bax mRNA 表达自骨折6 h 以后开始高于正常对照组(P <0.01)。在骨折治疗组,Bcl-2表达在1 h 明显比单纯骨折组高(P <0.01),Bcl-2 mRNA 表达在1~48 h 均高于单纯骨折组(P <0.01)；Bax 及 Bax mRNA 表达在1~48 h 均较单纯骨折组低(P <0.01)。结论骨折创伤对肾髓质浅层 Bcl-2、Bax 蛋白的表达及 mRNA 的转录均有明显影响。PNS 可能通过上调抑凋亡基因 Bcl-2以及下调促凋亡基因 Bax 的表达,从而起到抑制组织细胞凋亡的作用。
목적：연구삼칠총조감(PNS)대대서고골골절후신수질천층세포조망적영향급기대골절후신손상적보호작용。방법장102지 Wistar 대서수궤분위단순골절조(36지)、골절치료조(36지)、정상대조조(30지)；전량조대서분별재골절건모후1、6、12、24、36、48 h 시각처사6지,재상동시간단정상대조조처사5지。HE 염색관찰신수질적병리변화,TUNEL 염색관찰조망세포,면역조화급원위잡교기술검측 Bcl-2화 Bax 재신수질천층조직중적표체。결과단순골절조중위우신수질천층적신소관상피세포가견경도과립변성,간질소혈관경도확장어혈；골절치료조여단순골절조상비,병변정도교경。재단순골절조중,Bcl-2급 Bcl-2 mRNA 표체재1~36 h 명현고우정상대조조(P <0.01)；Bax 표체재12~36 h 교정상대조조고(P <0.01),Bax mRNA 표체자골절6 h 이후개시고우정상대조조(P <0.01)。재골절치료조,Bcl-2표체재1 h 명현비단순골절조고(P <0.01),Bcl-2 mRNA 표체재1~48 h 균고우단순골절조(P <0.01)；Bax 급 Bax mRNA 표체재1~48 h 균교단순골절조저(P <0.01)。결론골절창상대신수질천층 Bcl-2、Bax 단백적표체급 mRNA 적전록균유명현영향。PNS 가능통과상조억조망기인 Bcl-2이급하조촉조망기인 Bax 적표체,종이기도억제조직세포조망적작용。
Objective To study the effects of panax notoginseng saponins (PNS)on rat superficial renal medulla cells after femoral fracture and to discuss the protective role of PNS in renal injury after fracture. Methods We divided 102 Wistar rats randomly into simple fracture group (n =36),fracture and medication group (n =36)and normal control group (n =30).In the former two groups 6 rats were respectively executed at 1,6,12, 24,36,48 h after fracture modeling,while 5 rats in normal control group were executed at the corresponding time points.Regular HE staining was used to observe pathological changes and TUNEL was used for apoptotic cells.And immunohistochemistry and in situ hybridization were used to detect the expressions of Bcl-2 and Bax in superficial renal medulla tissues.Results In simple fracture group,mild granular degeneration could be seen in renal tubular epithelial cells of superficial renal medulla and the interstitial small vessels were slightly expanded and congested. Compared with simple fracture group,the lesions were slighter in fracture and medication group.In simple fracture group,Bcl-2 and Bcl-2 mRNA expressions were significantly higher than those in normal control group at 1 -36 h (P <0.01).Bax expression was higher than that in normal control group at 12-36 h (P <0.01),and Bax mRNA expression was higher than that in normal control group after 6 h after fracture (P < 0.01 ).In fracture and medication group,Bcl-2 expression was obviously higher than that in simple fracture group at 1 h (P <0.01),and Bcl-2 mRNA expression was higher than that in simple fracture group at 1 -48 h (P <0.01).Bax and Bax mRNA expressions were both lower than those in simple fracture group at 1 - 48 h (P < 0.01 ).Conclusion Fracture trauma has significant influences on protein expression and mRNA transcription of Bcl-2 and Bax in superficial renal medulla.PNS can upregulate anti-apoptosis gene Bcl-2 and downregulate pro-apoptosis gene Bax,thus playing the role of inhibiting tissue cell apoptosis.