西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2015年
4期
514-518
,共5页
党万太%王婧%谢文光%周京国
黨萬太%王婧%謝文光%週京國
당만태%왕청%사문광%주경국
痛风性关节炎%sCD14%炎症%白介素 1β(IL-1β)%肿瘤坏死因子α(TNF-α)%C 反应蛋白(CRP)
痛風性關節炎%sCD14%炎癥%白介素 1β(IL-1β)%腫瘤壞死因子α(TNF-α)%C 反應蛋白(CRP)
통풍성관절염%sCD14%염증%백개소 1β(IL-1β)%종류배사인자α(TNF-α)%C 반응단백(CRP)
gouty arthritis%soluble membrane CD14 (sCD14)%inflammation%interleukin 1β(IL-1β)%tumor necrosis factor-α(TNF-α)%C reaction protein (CRP)
目的：探讨可溶性白细胞分化抗原14(sCD14)在痛风性关节炎(GA)患者炎症反应中的变化及其意义。方法选取31例急性 GA(AGA)患者、23例非 AGA(NAGA)患者和20名健康对照者(HC),用实时荧光定量聚合酶链反应(qRT-PCR)检测其外周血单个核细胞(PBMCs)中 CD14的 mRNA 表达水平；用酶联免疫吸附法(ELISA)检测各组血浆 sCD14、白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)蛋白表达水平,用免疫比浊法测定各组血浆 C 反应蛋白(CRP)含量；检测各组白细胞计数(WBC)、嗜中性粒细胞绝对值(GR)、淋巴细胞绝对值(LY)、单核细胞绝对值(MO)等血常规及血尿酸(UA)等指标变化,比较3组指标的差异并分析其与 PBMCs 的 CD14 mRNA 表达及血浆 sCD14表达的相关性。结果AGA 组 PBMCs 中 CD14 mRNA 表达量高于 HC 组(P <0.05)；HC 与 NAGA 组血浆 sCD14蛋白表达低于 AGA 组(P <0.01),且 NAGA 组表达低于 HC 组(P <0.05)；AGA 组 CRP 蛋白水平高于 HC 与 NAGA 组(P <0.01)；AGA、NAGA 组血浆 IL-1β、TNF-α蛋白水平均显著高于 HC 组(P <0.01),且 AGA 组 IL-1β蛋白水平高于NAGA 组(P <0.01)；AGA 组和 NAGA 组 WBC 高于 HC 组(分别 P <0.01,P <0.05)；AGA 组 GR、MO 均高于 HC组(P <0.05),AGA 组 MO 高于 NAGA 组(P <0.05)；AGA 与 NAGA 组 UA 均显著高于 HC 组(P <0.01)。AGA组 PBMCs 的 CD14 mRNA 表达与 IL-1 b呈正相关(rs =0.362,P =0.045)；NAGA 组血浆 sCD14蛋白表达与 GR 呈正相关(rs =0.397,P =0.030)。结论sCD14在 GA 患者的炎症反应中表达异常,提示 sCD14在 GA 患者的炎症反应中可能发挥重要的调控作用。
目的：探討可溶性白細胞分化抗原14(sCD14)在痛風性關節炎(GA)患者炎癥反應中的變化及其意義。方法選取31例急性 GA(AGA)患者、23例非 AGA(NAGA)患者和20名健康對照者(HC),用實時熒光定量聚閤酶鏈反應(qRT-PCR)檢測其外週血單箇覈細胞(PBMCs)中 CD14的 mRNA 錶達水平；用酶聯免疫吸附法(ELISA)檢測各組血漿 sCD14、白介素1β(IL-1β)、腫瘤壞死因子α(TNF-α)蛋白錶達水平,用免疫比濁法測定各組血漿 C 反應蛋白(CRP)含量；檢測各組白細胞計數(WBC)、嗜中性粒細胞絕對值(GR)、淋巴細胞絕對值(LY)、單覈細胞絕對值(MO)等血常規及血尿痠(UA)等指標變化,比較3組指標的差異併分析其與 PBMCs 的 CD14 mRNA 錶達及血漿 sCD14錶達的相關性。結果AGA 組 PBMCs 中 CD14 mRNA 錶達量高于 HC 組(P <0.05)；HC 與 NAGA 組血漿 sCD14蛋白錶達低于 AGA 組(P <0.01),且 NAGA 組錶達低于 HC 組(P <0.05)；AGA 組 CRP 蛋白水平高于 HC 與 NAGA 組(P <0.01)；AGA、NAGA 組血漿 IL-1β、TNF-α蛋白水平均顯著高于 HC 組(P <0.01),且 AGA 組 IL-1β蛋白水平高于NAGA 組(P <0.01)；AGA 組和 NAGA 組 WBC 高于 HC 組(分彆 P <0.01,P <0.05)；AGA 組 GR、MO 均高于 HC組(P <0.05),AGA 組 MO 高于 NAGA 組(P <0.05)；AGA 與 NAGA 組 UA 均顯著高于 HC 組(P <0.01)。AGA組 PBMCs 的 CD14 mRNA 錶達與 IL-1 b呈正相關(rs =0.362,P =0.045)；NAGA 組血漿 sCD14蛋白錶達與 GR 呈正相關(rs =0.397,P =0.030)。結論sCD14在 GA 患者的炎癥反應中錶達異常,提示 sCD14在 GA 患者的炎癥反應中可能髮揮重要的調控作用。
목적：탐토가용성백세포분화항원14(sCD14)재통풍성관절염(GA)환자염증반응중적변화급기의의。방법선취31례급성 GA(AGA)환자、23례비 AGA(NAGA)환자화20명건강대조자(HC),용실시형광정량취합매련반응(qRT-PCR)검측기외주혈단개핵세포(PBMCs)중 CD14적 mRNA 표체수평；용매련면역흡부법(ELISA)검측각조혈장 sCD14、백개소1β(IL-1β)、종류배사인자α(TNF-α)단백표체수평,용면역비탁법측정각조혈장 C 반응단백(CRP)함량；검측각조백세포계수(WBC)、기중성립세포절대치(GR)、림파세포절대치(LY)、단핵세포절대치(MO)등혈상규급혈뇨산(UA)등지표변화,비교3조지표적차이병분석기여 PBMCs 적 CD14 mRNA 표체급혈장 sCD14표체적상관성。결과AGA 조 PBMCs 중 CD14 mRNA 표체량고우 HC 조(P <0.05)；HC 여 NAGA 조혈장 sCD14단백표체저우 AGA 조(P <0.01),차 NAGA 조표체저우 HC 조(P <0.05)；AGA 조 CRP 단백수평고우 HC 여 NAGA 조(P <0.01)；AGA、NAGA 조혈장 IL-1β、TNF-α단백수평균현저고우 HC 조(P <0.01),차 AGA 조 IL-1β단백수평고우NAGA 조(P <0.01)；AGA 조화 NAGA 조 WBC 고우 HC 조(분별 P <0.01,P <0.05)；AGA 조 GR、MO 균고우 HC조(P <0.05),AGA 조 MO 고우 NAGA 조(P <0.05)；AGA 여 NAGA 조 UA 균현저고우 HC 조(P <0.01)。AGA조 PBMCs 적 CD14 mRNA 표체여 IL-1 b정정상관(rs =0.362,P =0.045)；NAGA 조혈장 sCD14단백표체여 GR 정정상관(rs =0.397,P =0.030)。결론sCD14재 GA 환자적염증반응중표체이상,제시 sCD14재 GA 환자적염증반응중가능발휘중요적조공작용。
Objective To study the changes and significance of sCD14 in inflammatory response of patients with gouty arthritis.Methods CD14 mRNA was measured using quantitative real-time PCR in peripheral blood mononuclear cells (PBMCs).The expression of CD14 mRNA in PBMCs was compared between patients with acute gouty arthritis (AGA)(n =31)and non-acute gouty arthritis (NAGA)(n =23)and healthy controls (HC)(n =20).β-actin was selected as the internal control.The protein expressions of sCD14,IL-1βand TNF-αwere measured using enzyme linked immunosorbent assay (ELISA) in patients’ plasma.The protein expression of CRP was measured using immunoturbidimetry in patients’ plasma. Routine blood and blood biochemistry indexes were measured by routine blood analyzer and blood biochemistry analyzer of patients with AGA,NAGA and HC.We analyzed the correlation between CD14 mRNA,sCD14 protein expression and each clinical indicator.Results When compared with that in AGA group,the mRNA expression of CD14 increased significantly in PBMCs of HC patients (P < 0.05 ).When compared with that in HC and NAGA patients,the protein expression of sCD14 increased significantly in the plasma of AGA patients (P <0.01).The protein expression of sCD14 was significantly lower in the plasma of NAGA than in HC (P <0.05).The protein expression of sCD14 increased significantly in the plasma of AGA compared with HC and NAGA (P < 0.01 ).When compared with those in HC,the protein expressions of IL-1β and TNF-α increased significantly in the plasma of AGA and NAGA (P < 0.01 ).When compared with that in NAGA,the protein expression of IL-1βincreased significantly in plasma of AGA (P <0.01). The indexes of WBC increased significantly in AGA compared with HC (P <0.01),and WBC increased significantly in NAGA compared with HC (P <0.05).The indexes of GR and MO increased significantly in AGA compared with HC (P <0.05),and MO increased significantly in AGA compared with NAGA (P < 0.05 ).The indexes of UA increased significantly in AGA and NAGA compared with HC (P <0.01).There was a positive correlation between CD14 mRNA expression and IL-1β in PBMCs in AGA group (r s =0.362,P =0.045).A positive correlation was found between GR and the protein expression of sCD14 in NAGA patients’plasma (r s = 0.397,P = 0.030 ). Conclusion The dysregulated expressions of CD14 mRNA in PBMCs and sCD14 protein in GA show that sCD14 may play a significantly regulatory role in inflammatory reaction.
