CAJ | 학술논문

目的：探讨WW结构域氧化还原酶基因（WW domain-containing oxidoreductase gene,WWOX）与P73蛋白在皮肤恶性黑色素瘤（cutaneous malignant melanoma,CMM）中的表达及其意义。方法：采用免疫组化法（SP）检测66例CMM、30例交界痣和20例正常皮肤组织蜡块中的WWOX、P73蛋白表达水平。使用SPSSl7.0统计软件包，采用卡方检验和Spearman等级相关分析方法进行统计学分析，检验标准a=0.05，以P＜0.05为有统计学意义，χ2分割检验标准0.0167。结果：WWOX在正常皮肤的表达水平明显高于交界痣，其在交界痣的表达水平明显高于在CMM中的表达（P＜0.0167）；CMM中WWOX的表达水平随肿瘤浸润深度的增加而降低，伴有淋巴结转移者中的表达水平低于不伴有淋巴结转移者，并且差异均具有统计学差异（P＜0.05)。P73蛋白在正常皮肤的表达水平低于交界痣，其在交界痣的表达水平低于CMM（P＜0.0167）；CMM中的P73蛋白表达水平随肿瘤浸润深度的增加而增高，伴有淋巴结转移者中的表达水平高于不伴有淋巴结转移者中的表达水平（P＜0.05)。WWOX与P73蛋白在CMM中的表达呈负相关（r=－0.298,P=0.015）。结论：WWOX与P73共同参与调控CMM的发生发展，对其进行联合检测，有助于CMM的早期诊断，为临床治疗提供参考指标，同时也为CMM的基因治疗提供一个新思路。
목적：탐토WW결구역양화환원매기인（WW domain-containing oxidoreductase gene,WWOX）여P73단백재피부악성흑색소류（cutaneous malignant melanoma,CMM）중적표체급기의의。방법：채용면역조화법（SP）검측66례CMM、30례교계지화20례정상피부조직사괴중적WWOX、P73단백표체수평。사용SPSSl7.0통계연건포，채용잡방검험화Spearman등급상관분석방법진행통계학분석，검험표준a=0.05，이P＜0.05위유통계학의의，χ2분할검험표준0.0167。결과：WWOX재정상피부적표체수평명현고우교계지，기재교계지적표체수평명현고우재CMM중적표체（P＜0.0167）；CMM중WWOX적표체수평수종류침윤심도적증가이강저，반유림파결전이자중적표체수평저우불반유림파결전이자，병차차이균구유통계학차이（P＜0.05)。P73단백재정상피부적표체수평저우교계지，기재교계지적표체수평저우CMM（P＜0.0167）；CMM중적P73단백표체수평수종류침윤심도적증가이증고，반유림파결전이자중적표체수평고우불반유림파결전이자중적표체수평（P＜0.05)。WWOX여P73단백재CMM중적표체정부상관（r=－0.298,P=0.015）。결론：WWOX여P73공동삼여조공CMM적발생발전，대기진행연합검측，유조우CMM적조기진단，위림상치료제공삼고지표，동시야위CMM적기인치료제공일개신사로。
Objective To study the expression and correlation of WWOX and P73 protein in cutaneous malignant melanoma. Methods Retrospective method was used in the study.The expression level of WWOX and p73 was determined using immunohischemistry staining in 66 cases of cutaneous malignant melanoma,30 cases of junctional nevus and 20 cases of normal skin tissues.The results were analyzed using chi-squared test and Spearman rank correlation test.P<0.05 is considered statistically significant. P=0.0167 used for chi-squared test.All statistical analyses were performed using SPSS17.0. Results The positive rate of WWOX protein in CMM is significantly lower than that in junction nevus,and the positive rate in junction nevus is significantly lower than that in normal skin (P<0.0167),with the depth of tumor infiltration increasing,the expression of WWOX in CMM has decreased (P<0.05),the expression of WWOX in the tissue with lymph node metastasis is significantly lower than that without lymph node metastasis(P<0.05).The positive rate in normal skin was less lower than that in junction nevus ,and the positive rate in junction nevus was less lower than that in CMM(P<0.0167),with the depth of tumor infiltration increasing,the expression of P73 in CMM has increased(P<0.05),the expression level of P73 in the tissue with lymph node metastasis is significantly higher than that without lymph node metastasis(P<0.05). Conclusion WWOX and P73 might play an important role in occurrence and development of CMM,The combined detection of WWOX and P73 might be helpful for early diagnosis of CMM,to provide reference for clinical treatment and supply a new way for gene therapy of CMM.