西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2015年
4期
496-500
,共5页
陈亚丽%周丹丹%程红霞%张义长
陳亞麗%週丹丹%程紅霞%張義長
진아려%주단단%정홍하%장의장
高氧液%油酸性急性肺损伤%肺表面活性物质%表面蛋白 A
高氧液%油痠性急性肺損傷%肺錶麵活性物質%錶麵蛋白 A
고양액%유산성급성폐손상%폐표면활성물질%표면단백 A
hyperoxia solution%oleic-induced acute lung injury%pulmonary surfactant%surfactant protein A
目的：观察高氧液对急性油酸性肺损伤家兔肺组织形态改变及肺表面活性物质的影响。方法健康家兔30只,雌雄不拘,体质量2.0~2.5 kg,随机分为3组：空白对照组(n=10)；高氧液治疗组(n=10)；盐水对照组(n=10)。3组动物给予相应处理后分别在 ALI 模型制备前(基础值)、静脉输液前(损伤后)、输液后30 min、1 h、2 h 时观察动脉血气变化,2 h 后处死动物取肺组织做病理切片观察肺组织形态学改变和免疫组化检测肺表面活性物质的变化。结果高氧液治疗组在输注高氧液后2 h PaO2明显高于盐水对照组(P <0.001),而 PaCO2则低于盐水对照组(P <0.001)。光镜下高氧液治疗组家兔肺组织损伤程度较盐水对照组轻。免疫组化及肺泡灌洗液测定结果显示,高氧液治疗组肺表面活性物质 A 含量明显高于盐水对照组(P <0.001)。结论静脉输注高氧液可减轻肺组织损伤程度,减少肺泡表面活性物质的破坏。
目的：觀察高氧液對急性油痠性肺損傷傢兔肺組織形態改變及肺錶麵活性物質的影響。方法健康傢兔30隻,雌雄不拘,體質量2.0~2.5 kg,隨機分為3組：空白對照組(n=10)；高氧液治療組(n=10)；鹽水對照組(n=10)。3組動物給予相應處理後分彆在 ALI 模型製備前(基礎值)、靜脈輸液前(損傷後)、輸液後30 min、1 h、2 h 時觀察動脈血氣變化,2 h 後處死動物取肺組織做病理切片觀察肺組織形態學改變和免疫組化檢測肺錶麵活性物質的變化。結果高氧液治療組在輸註高氧液後2 h PaO2明顯高于鹽水對照組(P <0.001),而 PaCO2則低于鹽水對照組(P <0.001)。光鏡下高氧液治療組傢兔肺組織損傷程度較鹽水對照組輕。免疫組化及肺泡灌洗液測定結果顯示,高氧液治療組肺錶麵活性物質 A 含量明顯高于鹽水對照組(P <0.001)。結論靜脈輸註高氧液可減輕肺組織損傷程度,減少肺泡錶麵活性物質的破壞。
목적：관찰고양액대급성유산성폐손상가토폐조직형태개변급폐표면활성물질적영향。방법건강가토30지,자웅불구,체질량2.0~2.5 kg,수궤분위3조：공백대조조(n=10)；고양액치료조(n=10)；염수대조조(n=10)。3조동물급여상응처리후분별재 ALI 모형제비전(기출치)、정맥수액전(손상후)、수액후30 min、1 h、2 h 시관찰동맥혈기변화,2 h 후처사동물취폐조직주병리절편관찰폐조직형태학개변화면역조화검측폐표면활성물질적변화。결과고양액치료조재수주고양액후2 h PaO2명현고우염수대조조(P <0.001),이 PaCO2칙저우염수대조조(P <0.001)。광경하고양액치료조가토폐조직손상정도교염수대조조경。면역조화급폐포관세액측정결과현시,고양액치료조폐표면활성물질 A 함량명현고우염수대조조(P <0.001)。결론정맥수주고양액가감경폐조직손상정도,감소폐포표면활성물질적파배。
Objective To observe the effects of hyperoxia solution on pulmonary surfactant and histology of rabbits with oleic acid-induced acute lung injury.Methods Thirty healthy rabbits weighing 2.0 - 2.5 kg were randomly divided into three groups with 10 rabbits each:control group,hyperoxia solution treatment group,and saline treatment group.Blood samples were taken for blood-gas analysis before and at 30,60 and 120 min after oleic acid or normal saline administration.Two hours later,the animals were killed and pathologic changes of lung tissue were observed microscopically.The expression of SP-A was investigated using the immunohistochemical method and image analysis system.Results PaO2 was significantly higher in hyperoxia solution treatment group than in saline treatment group two hours after treatment (P < 0.001 ),but PaCO2 was significantly lower than that in saline treatment group (P <0.001).Optical microscopy showed that lung tissue damage was milder in hyperoxia solution treatment group than in saline treatment group.The content of surfactant A in hyperoxia solution treatment group was significantly greater than that in saline treatment group (P < 0.001 ).Conclusion Hyperoxia solution can lessen lung tissue injury and damage to pulmonary surfactant.
