CAJ | 학술논문

针对常规石蜡切片方法无法制得橡胶树木质部切片的问题，优化了橡胶树木质部切片制作方法并进行了验证评估。以3龄和30龄的橡胶树木材为材料，经FAA固定、乙醇预处理后，增加了以冰醋酸软化木质部这一步骤，用正丁醇取代二甲苯进行组织透明，其余步骤参考常规方法。结果表明，优化的石蜡切片方法可制作出完整、高质量的木质部切片，其木纤维的长度、宽度与硝酸离析所得到的木纤维一致度高，为橡胶树成龄木材的解剖学研究提供了方法。
침대상규석사절편방법무법제득상효수목질부절편적문제，우화료상효수목질부절편제작방법병진행료험증평고。이3령화30령적상효수목재위재료，경FAA고정、을순예처리후，증가료이빙작산연화목질부저일보취，용정정순취대이갑분진행조직투명，기여보취삼고상규방법。결과표명，우화적석사절편방법가제작출완정、고질량적목질부절편，기목섬유적장도、관도여초산리석소득도적목섬유일치도고，위상효수성령목재적해부학연구제공료방법。
In this paper, we improved and evaluated paraffin section method of Hevea xylem compared to the conventional method. 3-year-old and 30-year-old rubber tree woods were collected, fixed in FAA, pretreated by ethanol, softened by acetic acid and transparentized by n-butanol replacing xylene. The remaining steps referenced conventional method. The result indicated that the improved method could produce complete, high quality paraffin section of Hevea xylem. The length and width of wood fiber by paraffin section had high consistency with those obtained by nitrate segregation, which provided a method for the study of mature Hevea wood anatomy.