CAJ | 학술논문

背景：体细胞重新编程技术也称细胞重组技术，使已经完成分化的体细胞回到原始的全能性或多能性状态，并可以重新分化成与原来不一样的细胞。通过重编程技术可以获得患者特异性诱导多能干细胞和疾病特异性诱导多能干细胞，显著减少了免疫排斥反应。目的：探讨关于直接重编程到特定系的方法，总结参与重编程的分子机制。方法：以“重编程”为中文检索词，“reprogramming”为英文检索词，应用计算机检索维普(VIP)期刊全文数据库、万方全文数据库、中国知网全文数据库、PubMed 数据库、Springer 数据库1958年1月至2015年4月有关细胞重编程技术的文献，排除与研究目的无关及重复性研究，保留40篇文献进一步分析。结果与结论：当前重编程的步骤效率很低，在特定群只有相对少量的细胞能进行重编程，重编程的完整性和程度也有待证实。直接重编程成体、定系的细胞从一种细胞到另一种细胞一直是发育生物学很难达到的目标。最近的研究证明分化的细胞强制表达特异转录因子能促进细胞分化。这些发现使再生医学领域有了重大进展，可以提供替代细胞治疗各种再生紊乱。目前，基本的分子机制需要进一步阐明，在直接重编程被应用于临床之前还有许多问题需要解决。
배경：체세포중신편정기술야칭세포중조기술，사이경완성분화적체세포회도원시적전능성혹다능성상태，병가이중신분화성여원래불일양적세포。통과중편정기술가이획득환자특이성유도다능간세포화질병특이성유도다능간세포，현저감소료면역배척반응。목적：탐토관우직접중편정도특정계적방법，총결삼여중편정적분자궤제。방법：이“중편정”위중문검색사，“reprogramming”위영문검색사，응용계산궤검색유보(VIP)기간전문수거고、만방전문수거고、중국지망전문수거고、PubMed 수거고、Springer 수거고1958년1월지2015년4월유관세포중편정기술적문헌，배제여연구목적무관급중복성연구，보류40편문헌진일보분석。결과여결론：당전중편정적보취효솔흔저，재특정군지유상대소량적세포능진행중편정，중편정적완정성화정도야유대증실。직접중편정성체、정계적세포종일충세포도령일충세포일직시발육생물학흔난체도적목표。최근적연구증명분화적세포강제표체특이전록인자능촉진세포분화。저사발현사재생의학영역유료중대진전，가이제공체대세포치료각충재생문란。목전，기본적분자궤제수요진일보천명，재직접중편정피응용우림상지전환유허다문제수요해결。
BACKGROUND:Somatic cel reprogramming technology, also known as recombinant technology, has completed differentiated somatic cels back to the original totipotent or pluripotent state, and can be re-differentiated into cels different from original ones. Re-programming techniques are able to harvest specificaly induced pluripotent stem cels and disease-specific induced pluripotent stem cels from patients, which can significantly reduce the immune rejection. OBJECTIVE: To explore the method from direct reprogramming to specific cel lines and to conclude the molecular mechanisms underlying reprogramming. METHODS:A computer-based search of VIP, Wanfang, CNKI, PubMed and Springer databases was performed for articles related to cel reprogramming techniques published from January 1958 to April 2015 using the keywords of “reprogramming” in Chinese and English, respectively. After elimination of unrelated and repetitive studies, 40 articles were retained for further analysis. RESULTS AND CONCLUSION:Current reprogramming steps are inefficient that in the specific group only a relatively smal number of cels can be reprogrammed, and the extent and integrity of reprogramming are questionable. Direct reprogramming of adult cels and specific cel lines that are changed to another line is always a difficulty in developmental biology. Recent studies have proved that differentiated cels can forcibly express specific transcription factor to improve cel differentiation. This discovery is a great progress in regenerative medicine that cel replacement therapy can be provided for renewable disorders. At present, the basic molecular mechanisms require further clarification, and there are many problems to be solved before the direct reprogramming is used clinicaly.