CAJ | 학술논문

背景：免疫炎症反应促使大量炎症因子的产生和释放是继发性脊髓损伤的其对主要原因。目的：探讨脐带沃顿胶干细胞移植对急性脊髓损伤大鼠的神经修复作用及其对炎症因子单核细胞趋化蛋白1和白细胞介素10表达的影响。方法：81只健康成年雄性SD大鼠，随机分为假手术组、模型组和脐带沃顿胶干细胞移植组(n=27)，后两组以脊髓半切方法建立急性脊髓损伤模型，造模后脐带沃顿胶干细胞移植组经尾静脉移植1×106个脐带沃顿胶干细胞。移植后不同时间通过BBB评分评价各组大鼠的运动功能；ELISA检测不同时间点各组大鼠血清中单核细胞趋化蛋白1的含量；qRT-PCR和Western分析不同时间点损伤脊髓组织中单核细胞趋化蛋白1及白细胞介素10的表达；免疫组织化学检测损伤组织中脐带沃顿胶干细胞的迁移和神经分化情况。结果与结论：与假手术组和模型组相比，脐带沃顿胶干细胞移植组大鼠的神经功能明显恢复(P <0.05)。脐带沃顿胶干细胞移植组大鼠血清中单核细胞趋化蛋白1水平显著低于模型组(P <0.05)。脐带沃顿胶干细胞移植组脊髓组织单核细胞趋化蛋白1 mRNA和蛋白表达显著低于模型组(P <0.05)，白细胞介素10 mRNA和蛋白表达显著高于模型组(P <0.05)。移植组中脐带沃顿胶干细胞可迁移至损伤部位，并表达神经胶质纤维酸性蛋白。这些结果提示脐带沃顿胶干细胞可能通过调控脊髓损伤组织的炎症反应，促进神经修复，这也可能是脐带沃顿胶干细胞治疗脊髓损伤的机制之一。
배경：면역염증반응촉사대량염증인자적산생화석방시계발성척수손상적기대주요원인。목적：탐토제대옥돈효간세포이식대급성척수손상대서적신경수복작용급기대염증인자단핵세포추화단백1화백세포개소10표체적영향。방법：81지건강성년웅성SD대서，수궤분위가수술조、모형조화제대옥돈효간세포이식조(n=27)，후량조이척수반절방법건립급성척수손상모형，조모후제대옥돈효간세포이식조경미정맥이식1×106개제대옥돈효간세포。이식후불동시간통과BBB평분평개각조대서적운동공능；ELISA검측불동시간점각조대서혈청중단핵세포추화단백1적함량；qRT-PCR화Western분석불동시간점손상척수조직중단핵세포추화단백1급백세포개소10적표체；면역조직화학검측손상조직중제대옥돈효간세포적천이화신경분화정황。결과여결론：여가수술조화모형조상비，제대옥돈효간세포이식조대서적신경공능명현회복(P <0.05)。제대옥돈효간세포이식조대서혈청중단핵세포추화단백1수평현저저우모형조(P <0.05)。제대옥돈효간세포이식조척수조직단핵세포추화단백1 mRNA화단백표체현저저우모형조(P <0.05)，백세포개소10 mRNA화단백표체현저고우모형조(P <0.05)。이식조중제대옥돈효간세포가천이지손상부위，병표체신경효질섬유산성단백。저사결과제시제대옥돈효간세포가능통과조공척수손상조직적염증반응，촉진신경수복，저야가능시제대옥돈효간세포치료척수손상적궤제지일。
BACKGROUND:The production and release of a large amount of inflammatory factors caused by immune system inflammatory response mainly contributes to secondary spinal cord injury. OBJECTIVE:To investigate the effects of umbilical cord Wharton’s jely mesenchymal stem cel transplantation on repair of injured neurological function and expression of inflammatory factors monocyte chemoattractant protein 1 and interleukin 10 in rats with acute spinal cord injury. METHODS: Eighty-one healthy adult male Sprague-Dawley rats were randomly and equaly divided into sham operation, model and cel transplantation groups, with 27 rats per group. Rats in the latter two groups were subjected to hemisection of the spinal cord to establish acute spinal cord injury models. Rat models in the cel transplantation group received umbilical cord Wharton’s jely mesenchymal stem cel injection (1×106)via the tail vein. Rat neurological function was evaluated using the BBB score at different time points after spinal cord injury. The expression of monocyte chemoattractant protein 1 and interleukin 10 in injured spinal cord tissue was detected using ELISA assay at different time points after spinal cord injury. Migration and neuronal differentiation of umbilical cord Wharton’s jely mesenchymal stem cels in the injured spinal cord tissue were determined using immunohistochemical staining method. RESULTS AND CONCLUSION:Compared with the sham operation and model groups, rat neurological function was significantly recovered in the cel transplantation group (P < 0.05). Compared to the model group, monocyte chemoattractant protein 1 level in the serum and monocyte chemoattractant protein 1 mRNA and protein expression in the injured spinal cord tissue were significantly lower (P < 0.05), but interleukin 10 mRNA and protein expression in the injured spinal cord tissue was significantly higher (P < 0.05), in the cel transplantation group. In the cel transplantation group, umbilical cord Wharton’s jely mesenchymal stem cels could migrate to the injured region and express glial fibrilary acidic protein. These findings suggest that umbilical cord Wharton’s jely mesenchymal stem cels promote rat neurological function recovery by regulating the inflammatory response in the injured spinal cord tissue, which is likely to be one of mechanisms by which transplantation of umbilical cord Wharton’s jely mesenchymal stem cels treats spinal cord injury.