CAJ | 학술논문

目的：建立测定枇芩颗粒剂中总黄酮含量的紫外分光光度法。方法以芦丁为对照品，采用70％的乙醇溶液加热回流提取总黄酮，于509 nm波长处测定总黄酮含量。结果芦丁质量浓度在12.72~76.32 mg/L范围内与吸光度有良好的线性关系，回归方程为A=0.019 C＋0.0107（ r=0.9995）。结论该法操作简便、结果可靠，可为枇芩颗粒剂的质量控制提供依据。
목적：건립측정비금과립제중총황동함량적자외분광광도법。방법이호정위대조품，채용70％적을순용액가열회류제취총황동，우509 nm파장처측정총황동함량。결과호정질량농도재12.72~76.32 mg/L범위내여흡광도유량호적선성관계，회귀방정위A=0.019 C＋0.0107（ r=0.9995）。결론해법조작간편、결과가고，가위비금과립제적질량공제제공의거。
Objective To establish a UV spectrophotometry method for determining the content of total flavonoids from Piqin Granules. Methods With rutin as the reference substance, total flavonoids were extracted from Piqin Granules by the 70% ethanol solution heat-ing reflux extraction. Then, the content of total flavonoids was detected at 509 nm by UV Spectrophotometry. Results The rutin mass concentration in the range of 12. 72-76. 32 mg/L showed the good linear relationship with absorbancy. The regression equation was A=0. 019 C+0. 010 7 ( r=0. 999 5 ) . Conclusion This method is simple to operate and reliable in the detection results, and can pro-vide the basis for the quality control of Piqin Granules.