CAJ | 학술논문

目的：建立测定橙皮苷磷脂复合物中橙皮苷含量的高效液相色谱（HPLC）法。方法色谱柱为Diamonsil C18柱（250 mm ×4.6 mm，5μm），流动相为乙腈－0.5％醋酸水溶液(25：75)，流速为1.0 mL/min，检测波长283 nm，柱温30℃，进样量20μL。结果橙皮苷进样质量浓度在5.19~103.73μg/mL ( r=0.9995)范围内与峰面积线性关系良好，平均回收率为101.02％( RSD=1.30％)，橙皮苷磷脂复合物中橙皮苷的平均含量为282.5μg/mg。结论该法准确可靠、重复性好，可用于橙皮苷磷脂复合物的含量测定。
목적：건립측정등피감린지복합물중등피감함량적고효액상색보（HPLC）법。방법색보주위Diamonsil C18주（250 mm ×4.6 mm，5μm），류동상위을정－0.5％작산수용액(25：75)，류속위1.0 mL/min，검측파장283 nm，주온30℃，진양량20μL。결과등피감진양질량농도재5.19~103.73μg/mL ( r=0.9995)범위내여봉면적선성관계량호，평균회수솔위101.02％( RSD=1.30％)，등피감린지복합물중등피감적평균함량위282.5μg/mg。결론해법준학가고、중복성호，가용우등피감린지복합물적함량측정。
Objective To establish a HPLC method for the content determination of hesperidin-phospholipid complex. Methods The chromatographic separation was performed on a Diamonsil C18 column ( 250 mm × 4. 6 mm, 5 μm ) with a mobile phase of acetonitrile-0. 5% acetic acid aqueous solution ( 25 :75 ) . The flow rate was 1. 0 mL/min and the detection wavelength was 283 nm. The column temperature was maintained at 30 ℃ and the inject volume was 20 μL. Results Hesperidin in the range of 5. 19-103. 73 μg/mL showed the good linear relation ( r=0. 999 5 ) . The average recovery rate was 101. 02% ( RSD=1. 30% ) . The average content of hes-peridin contained in hesperidin-phospholipid complex was 282. 5 μg/mg. Conclusion The established method in this study is accu-rate, reliable and reproducible, and can be used for the content determination of hesperidin-phospholipid complex.