中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2015年
18期
39-40
,共2页
李方知%黄广雄%卢峰岳
李方知%黃廣雄%盧峰嶽
리방지%황엄웅%로봉악
结核性胸积液%腺苷脱氨酶%同工酶%腺苷脱氨酶2%免疫组化
結覈性胸積液%腺苷脫氨酶%同工酶%腺苷脫氨酶2%免疫組化
결핵성흉적액%선감탈안매%동공매%선감탈안매2%면역조화
Tuberculosis pleural effusion%Adenosine deaminase%Isoenzymes%Adenosine deaminase 2%Immunohistochemistry
目的：研究腺苷脱氨酶2（Adenosine deaminase 2，ADA2）在结核性胸积液总ADA活性所占比例，及其在结核性胸积液胸膜组织中的细胞来源。方法：测定确诊的32例x结核性胸积液ADA2的总ADA和ADA2活性，以ADA2抗体为一抗，用免疫组化方法检测ADA2在结核性胸积液胸膜组织中的细胞来源。结果：ADA2活性占总ADA的74.8%。结核性胸积液胸膜组织的ADA2免疫组化，可见朗格汉斯巨细胞、上皮样细胞和巨噬细胞被深染，胞浆和胞膜有大量的棕黄色沉着。结论：结核性胸积液的ADA活性主要由ADA2构成，ADA2源自巨噬细胞、上皮样细胞和朗格汉斯巨细胞。
目的：研究腺苷脫氨酶2（Adenosine deaminase 2，ADA2）在結覈性胸積液總ADA活性所佔比例，及其在結覈性胸積液胸膜組織中的細胞來源。方法：測定確診的32例x結覈性胸積液ADA2的總ADA和ADA2活性，以ADA2抗體為一抗，用免疫組化方法檢測ADA2在結覈性胸積液胸膜組織中的細胞來源。結果：ADA2活性佔總ADA的74.8%。結覈性胸積液胸膜組織的ADA2免疫組化，可見朗格漢斯巨細胞、上皮樣細胞和巨噬細胞被深染，胞漿和胞膜有大量的棕黃色沉著。結論：結覈性胸積液的ADA活性主要由ADA2構成，ADA2源自巨噬細胞、上皮樣細胞和朗格漢斯巨細胞。
목적：연구선감탈안매2（Adenosine deaminase 2，ADA2）재결핵성흉적액총ADA활성소점비례，급기재결핵성흉적액흉막조직중적세포래원。방법：측정학진적32례x결핵성흉적액ADA2적총ADA화ADA2활성，이ADA2항체위일항，용면역조화방법검측ADA2재결핵성흉적액흉막조직중적세포래원。결과：ADA2활성점총ADA적74.8%。결핵성흉적액흉막조직적ADA2면역조화，가견랑격한사거세포、상피양세포화거서세포피심염，포장화포막유대량적종황색침착。결론：결핵성흉적액적ADA활성주요유ADA2구성，ADA2원자거서세포、상피양세포화랑격한사거세포。
Objective:To investigate the proportion of Adenosine deaminase 2(ADA2) in total ADA activity of the tuberculosis pleural effusion and its cellular source in pleural tissue.Method:The total ADA and ADA2 activity from thirty-two tuberculosis patients’ effusions were measured.The cellular source of ADA2 were examined by immunohistochemistry method using CECR1 antibody.Result:The ADA2 contributed 74.8% to the total ADA.The immunohistochemistry of ADA2 in the pleural tissues of tuberculous pleural effusion showed that langhans cells,epithelioid cells and macrophages were stained deeply.Their cytoplasm and membrane were deposited by yellow granules.Conclusion:ADA2 is primarily responsible for total ADA activity in tuberculosis pleural effusion and it is secreted by macrophages,epithelioid cells and langhans cells.
