中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
23期
3686-3691
,共6页
干细胞%移植%人端粒酶反转录酶%脐带脐血干细胞%基因修饰%脐带间充质干细胞%大鼠%急性肾损伤%修复%端粒酶反转录酶%干细胞移植%肾损伤
榦細胞%移植%人耑粒酶反轉錄酶%臍帶臍血榦細胞%基因脩飾%臍帶間充質榦細胞%大鼠%急性腎損傷%脩複%耑粒酶反轉錄酶%榦細胞移植%腎損傷
간세포%이식%인단립매반전록매%제대제혈간세포%기인수식%제대간충질간세포%대서%급성신손상%수복%단립매반전록매%간세포이식%신손상
Tissue Engineering%Stem Cells%Umbilical Cord Mesenchymal Stem Cells%Rats%Renal Injury
背景：人端粒酶反转录酶(hTERT)是调控增殖及定向分化的首选生长因子之一，具有多重生物学效应，为建立基因工程的永生化干细胞系奠定了基础。目的：探讨人端粒酶反转录酶基因修饰脐带间充质干细胞移植对大鼠缺血再灌注诱导的急性肾损伤的治疗作用。方法：体外培养人脐带间充质干细胞，构建缺血再灌注诱导的大鼠急性肾损伤模型，建模后将大鼠随机分为3组：对照组尾静脉注射1 mL L-DMEM培养液；空载病毒组：尾静脉注射1 mL经空载病毒转染人脐带间充质干细胞悬液；hTERT转染组尾静脉注射1 mL经PLXSN-hTERT转染的人脐带间充质干细胞悬液。结果与结论：移植后第3，28天苏木精-伊红染色检查示hTERT转染组的肾小管损伤评分<空载病毒组<对照组(P <0.05)。移植后第28天，CM-Dil阳性细胞数为hTERT转染组>空载病毒组>对照组(P <0.05)。移植细胞后第1，3，14，28天血肌酐、尿素氮水平均为hTERT转染组<空载病毒组<对照组(P <0.05)。结果证实，hTERT基因修饰脐带间充质干细胞移植对大鼠急性肾损伤具有明显的修复作用。
揹景：人耑粒酶反轉錄酶(hTERT)是調控增殖及定嚮分化的首選生長因子之一，具有多重生物學效應，為建立基因工程的永生化榦細胞繫奠定瞭基礎。目的：探討人耑粒酶反轉錄酶基因脩飾臍帶間充質榦細胞移植對大鼠缺血再灌註誘導的急性腎損傷的治療作用。方法：體外培養人臍帶間充質榦細胞，構建缺血再灌註誘導的大鼠急性腎損傷模型，建模後將大鼠隨機分為3組：對照組尾靜脈註射1 mL L-DMEM培養液；空載病毒組：尾靜脈註射1 mL經空載病毒轉染人臍帶間充質榦細胞懸液；hTERT轉染組尾靜脈註射1 mL經PLXSN-hTERT轉染的人臍帶間充質榦細胞懸液。結果與結論：移植後第3，28天囌木精-伊紅染色檢查示hTERT轉染組的腎小管損傷評分<空載病毒組<對照組(P <0.05)。移植後第28天，CM-Dil暘性細胞數為hTERT轉染組>空載病毒組>對照組(P <0.05)。移植細胞後第1，3，14，28天血肌酐、尿素氮水平均為hTERT轉染組<空載病毒組<對照組(P <0.05)。結果證實，hTERT基因脩飾臍帶間充質榦細胞移植對大鼠急性腎損傷具有明顯的脩複作用。
배경：인단립매반전록매(hTERT)시조공증식급정향분화적수선생장인자지일，구유다중생물학효응，위건립기인공정적영생화간세포계전정료기출。목적：탐토인단립매반전록매기인수식제대간충질간세포이식대대서결혈재관주유도적급성신손상적치료작용。방법：체외배양인제대간충질간세포，구건결혈재관주유도적대서급성신손상모형，건모후장대서수궤분위3조：대조조미정맥주사1 mL L-DMEM배양액；공재병독조：미정맥주사1 mL경공재병독전염인제대간충질간세포현액；hTERT전염조미정맥주사1 mL경PLXSN-hTERT전염적인제대간충질간세포현액。결과여결론：이식후제3，28천소목정-이홍염색검사시hTERT전염조적신소관손상평분<공재병독조<대조조(P <0.05)。이식후제28천，CM-Dil양성세포수위hTERT전염조>공재병독조>대조조(P <0.05)。이식세포후제1，3，14，28천혈기항、뇨소담수평균위hTERT전염조<공재병독조<대조조(P <0.05)。결과증실，hTERT기인수식제대간충질간세포이식대대서급성신손상구유명현적수복작용。
BACKGROUND:The human telomerase reverse transcriptase (hTERT) is one of preferred growth factors for regulating proliferation and directional differentiation, has multiple biological effects, and laids the foundation for geneticaly engineered immortalized stem cel lines. OBJECTIVE: To investigate the effect ofhTERT gene-modified umbilical cord mesenchymal stem cel transplantation on acute kidney injury induced by ischemia and reperfusion in rats. METHODS:The human umbilical cord mesenchymal stem cels were cultured in vitro. Rat models of acute kidney injury induced by ischemia and reperfusion were established. Rat models were randomly divided into three groups. Rats in the control group were injected with 1 mL L-DMEM medium through caudal vein. Rats in the negative transfection group were injected with 1 mL umbilical cord mesenchymal stem cel suspension after empty virus transfection through caudal vein. Rats in the hTERT transfection group were injected with 1 mL umbilical cord mesenchymal stem cel suspension after PLXSN-hTERT transfection through caudal vein. RESULTS AND CONCLUSION:At 3 and 28 days after transplantation, hematoxylin-eosin staining showed renal tubular damage score in the hTERT transfection group < negative transfection group < control group (P < 0.05). At 28 days after transplantation, the number of CM-Dil-positive cels in the hTERT transfection group > negative transfection group > control group (P < 0.05). At 1, 3, 14, and 28 days, serum creatinine and urea nitrogen levels in the hTERT transfection group < negative transfection group < control group (P < 0.05). The results confirm that hTERT gene-modified umbilical cord mesenchymal stem cel transplantation has a significant repair effect on acute kidney injury in rats.
