CAJ | 학술논문

背景：干细胞制备完成后的放置时间与其存活率的关系是临床应用安全、有效的基础。目的：观察不同保存液及不同保存时间对脐带源间充质干细胞存活率的影响，为干细胞有效期的确定提供重要依据。方法：选用脐带源间充质干细胞制备干细胞制剂，分别采用生理盐水、培养基、培养基+生理盐水、生理盐水含表皮生长因子、培养基含低分子肝素钙悬浮保存。使用冷藏箱模拟细胞运输条件，分别在第0，6，12，18，24，30，36，42，48小时取样，进行细胞总数和细胞活率的检测。结果与结论：各组在24 h内的细胞数量和细胞活率差别不大。24 h后，培养基组和培养基含低分子肝素钙组的细胞总数和细胞活率优于生理盐水含因子组、生理盐水组、培养基+生理盐水组。干细胞制剂制备完成后，冷藏运输24 h内细胞活率可达90%以上，保存液中的营养成分及合适的pH值，可使细胞活率显著提高。
배경：간세포제비완성후적방치시간여기존활솔적관계시림상응용안전、유효적기출。목적：관찰불동보존액급불동보존시간대제대원간충질간세포존활솔적영향，위간세포유효기적학정제공중요의거。방법：선용제대원간충질간세포제비간세포제제，분별채용생리염수、배양기、배양기+생리염수、생리염수함표피생장인자、배양기함저분자간소개현부보존。사용랭장상모의세포운수조건，분별재제0，6，12，18，24，30，36，42，48소시취양，진행세포총수화세포활솔적검측。결과여결론：각조재24 h내적세포수량화세포활솔차별불대。24 h후，배양기조화배양기함저분자간소개조적세포총수화세포활솔우우생리염수함인자조、생리염수조、배양기+생리염수조。간세포제제제비완성후，랭장운수24 h내세포활솔가체90%이상，보존액중적영양성분급합괄적pH치，가사세포활솔현저제고。
BACKGROUND:The relationship between placing time after stem cel preparation and cel survival is the basis of safety and effectiveness for the clinical application. OBJECTIVE: To observe effects of different preservation solutions and different storage time on survival rate of umbilical cord-derived mesenchymal stem cels, and to provide important evidence for identifying effectiveness of stem cels. METHODS:Umbilical cord-derived mesenchymal stem cels were selected to prepare stem cel preparation, which was preserved in physiological saline, medium, medium+physiological saline, physiological saline containing epidermal growth factor, and medium containing low molecular heparin calcium suspension. Cold closet was selected for imitating celular transport conditions. Samples were obtained at 0, 6, 12, 18, 24, 30, 36, 42 and 48 hours. Total cel number and cel survival rate were detected. RESULTS AND CONCLUSION:The difference in cel number and survival rate was not great within 24 hours in each group. Twenty-four hours later, total cel number and survival rate were better in the medium and medium containing low molecular heparin calcium groups than in the physiological saline containing epidermal growth factor, physiological saline, and medium+physiological saline groups. These findings suggest that after stem cel preparation, the cel survival rate can reach more than 90% within 24 hours under refrigerated transport conditions. Nutritional ingredients and proper pH value of preservation solution can make the cel survival rate increased greatly.