中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
23期
3644-3651
,共8页
干细胞%脐带脐血干细胞%肝样细胞%脐带间充质干细胞%肝损伤%血清%诱导分化
榦細胞%臍帶臍血榦細胞%肝樣細胞%臍帶間充質榦細胞%肝損傷%血清%誘導分化
간세포%제대제혈간세포%간양세포%제대간충질간세포%간손상%혈청%유도분화
Umbilical Cord%Mesenchymal Stem Cells%Carbon Tetrachloride%Liver Diseases%Serum%Cell Differentiation%Hepatocytes
背景：脐带间充质干细胞来源于新生个体脐带组织，来源广泛，无社会伦理学因素制约，有望代替骨髓间充质干细胞成为细胞移植和再生医学的理想种子细胞。目的：探讨肝衰竭大鼠血清对人脐带间充质干细胞肝向诱导分化作用，为临床上利用脐带间充质干细胞治疗终末期肝病提供实验依据。方法：腹腔注射10%四氯化碳溶液建立急性肝损伤大鼠模型，对照组腹腔注射等剂量的大豆油，48 h后腹主动脉取血离心分离血清。取第3代人脐带间充质干细胞，分别用体积分数为20%肝损伤大鼠血清和体积分数为20%胎牛血清诱导培养，观察诱导前后人脐带间充质干细胞形态学改变，检测培养液上清甲胎蛋白、白蛋白水平。结果与结论：肝损伤大鼠血清培养第1天细胞形态变化不大，呈梭形，仍呈编织状或漩涡状生长，第2天细胞呈短梭形，第3天细胞呈类圆形，第4天呈圆形，并有极少量细胞漂浮。肝损伤大鼠血清培养人脐带间充质干细胞4 d，其上清液白蛋白水平较诱导前和对照组均有升高趋势(P <0.001)，甲胎蛋白水平无明显变化。结果表明肝损伤大鼠血清可使脐带间充质干细胞向肝样细胞分化。
揹景：臍帶間充質榦細胞來源于新生箇體臍帶組織，來源廣汎，無社會倫理學因素製約，有望代替骨髓間充質榦細胞成為細胞移植和再生醫學的理想種子細胞。目的：探討肝衰竭大鼠血清對人臍帶間充質榦細胞肝嚮誘導分化作用，為臨床上利用臍帶間充質榦細胞治療終末期肝病提供實驗依據。方法：腹腔註射10%四氯化碳溶液建立急性肝損傷大鼠模型，對照組腹腔註射等劑量的大豆油，48 h後腹主動脈取血離心分離血清。取第3代人臍帶間充質榦細胞，分彆用體積分數為20%肝損傷大鼠血清和體積分數為20%胎牛血清誘導培養，觀察誘導前後人臍帶間充質榦細胞形態學改變，檢測培養液上清甲胎蛋白、白蛋白水平。結果與結論：肝損傷大鼠血清培養第1天細胞形態變化不大，呈梭形，仍呈編織狀或漩渦狀生長，第2天細胞呈短梭形，第3天細胞呈類圓形，第4天呈圓形，併有極少量細胞漂浮。肝損傷大鼠血清培養人臍帶間充質榦細胞4 d，其上清液白蛋白水平較誘導前和對照組均有升高趨勢(P <0.001)，甲胎蛋白水平無明顯變化。結果錶明肝損傷大鼠血清可使臍帶間充質榦細胞嚮肝樣細胞分化。
배경：제대간충질간세포래원우신생개체제대조직，래원엄범，무사회윤리학인소제약，유망대체골수간충질간세포성위세포이식화재생의학적이상충자세포。목적：탐토간쇠갈대서혈청대인제대간충질간세포간향유도분화작용，위림상상이용제대간충질간세포치료종말기간병제공실험의거。방법：복강주사10%사록화탄용액건립급성간손상대서모형，대조조복강주사등제량적대두유，48 h후복주동맥취혈리심분리혈청。취제3대인제대간충질간세포，분별용체적분수위20%간손상대서혈청화체적분수위20%태우혈청유도배양，관찰유도전후인제대간충질간세포형태학개변，검측배양액상청갑태단백、백단백수평。결과여결론：간손상대서혈청배양제1천세포형태변화불대，정사형，잉정편직상혹선와상생장，제2천세포정단사형，제3천세포정류원형，제4천정원형，병유겁소량세포표부。간손상대서혈청배양인제대간충질간세포4 d，기상청액백단백수평교유도전화대조조균유승고추세(P <0.001)，갑태단백수평무명현변화。결과표명간손상대서혈청가사제대간충질간세포향간양세포분화。
BACKGROUND:Umbilical cord mesenchymal stem cels are from the umbilical cord of newly born individuals and have no ethical issues, and therefore are promising candidates for seeded cels as a substitute for cel transplantation and regenerative medicine. OBJECTIVE:To investigate the effects of serum from liver injury rats on induced differentiation of human umbilical cord mesenchymal stem cels into hepatocyte-like cels and provide experimental evidence for use of human umbilical cord mesenchymal stem cels in the treatment of patients with end-stage liver disease in the clinic. METHODS: Rat models of acute liver injury were established by intraperitoneal injection of 10% carbon tetrachloride. Rats in the control group were intraperitonealy administered the same amount of soybean oil. Forty-eight hours after modeling, abdominal aorta blood was taken for serum preparation. Passage 3 human umbilical cord mesenchymal stem cels were cultured with 20% serum from liver injury rats and 20% fetal bovine serum. Morphology of human umbilical cord mesenchymal stem cels was observed before and after culture. Levels ofα-fetoprotein and albumin in the supernatant were detected. RESULTS AND CONCLUSION:Cels exhibited shuttle-shaped appearance and grew in whirlpool-like manner at 1 day after culture with serum from liver injury rats, exhibited short shuttle-shaped appearance at 2 days, were oval-shaped at 3 days, and were round and an extremely smal number of cels were floated at 4 days. At 4 days after culture with serum from liver injury rats, level of albumin in the cel supernatant was significantly increased than that before induction and that in the control group (P< 0.001), and there was no significant difference in level of α-fetoprotein in the cel supernatant. These results suggest that serum of liver injury rats can induce differentiation of umbilical cord mesenchymal stem cels into hepatocyte-like cels.
