CAJ | 학술논문

目的：探讨高脂饮食对大鼠甜味觉敏感性的影响。方法刚断乳雄性 SD 大鼠,适应1周后,高脂饮食喂养2周,按体质量增加程度将实验动物分为3组,即肥胖易感组(obesity prone,OP)、肥胖抵抗(obesity resistant,OR)组和对照组(control,C)。OP及 OR组继续喂养高脂饲料,对照组转由正常饲料喂养(C-C),连续8周。采用条件性厌味(CTA)及双瓶选择实验,检测大鼠对甜味的喜好及其阈值变化,并测定大鼠热量总摄入量和食物转化效率。结果①高脂喂养的OP大鼠体质量增加明显高于OR及对照组大鼠,而对照组大鼠体质量增加又高于高脂喂养的OR大鼠。②高脂喂养的大鼠(OP与OR)的蔗糖阈值明显低于正常对照组大鼠,但OP与OR大鼠之间的蔗糖阈值无差异。③高脂喂养大鼠(OP与 OR)的蔗糖喜好明显高于正常对照组大鼠,而 OP与 OR大鼠之间的蔗糖喜好无差异。④高脂喂养的 OP大鼠总热量摄入明显高于 OR 大鼠,但高脂饲料喂养与正常饲料喂养的大鼠总热量摄入无差异。⑤高脂喂养的 OP 大鼠食物效率明显高于 OR大鼠及正常对照大鼠,而高脂喂养的 OR大鼠食物效率与正常对照大鼠无差异。结论高脂饮食可通过增高大鼠甜味敏感性,从而影响摄食行为并引起肥胖。
목적：탐토고지음식대대서첨미각민감성적영향。방법강단유웅성 SD 대서,괄응1주후,고지음식위양2주,안체질량증가정도장실험동물분위3조,즉비반역감조(obesity prone,OP)、비반저항(obesity resistant,OR)조화대조조(control,C)。OP급 OR조계속위양고지사료,대조조전유정상사료위양(C-C),련속8주。채용조건성염미(CTA)급쌍병선택실험,검측대서대첨미적희호급기역치변화,병측정대서열량총섭입량화식물전화효솔。결과①고지위양적OP대서체질량증가명현고우OR급대조조대서,이대조조대서체질량증가우고우고지위양적OR대서。②고지위양적대서(OP여OR)적자당역치명현저우정상대조조대서,단OP여OR대서지간적자당역치무차이。③고지위양대서(OP여 OR)적자당희호명현고우정상대조조대서,이 OP여 OR대서지간적자당희호무차이。④고지위양적 OP대서총열량섭입명현고우 OR 대서,단고지사료위양여정상사료위양적대서총열량섭입무차이。⑤고지위양적 OP 대서식물효솔명현고우 OR대서급정상대조대서,이고지위양적 OR대서식물효솔여정상대조대서무차이。결론고지음식가통과증고대서첨미민감성,종이영향섭식행위병인기비반。
Objective To investigate the effect of high-fat diet on the sweet taste sensitivity in rats. Methods We subjected weaning male SD rats to the new feeding environment for 1 week and then fed them with high-fat diet for 2 weeks.According to the degree of weight gain,the rats were divided into obesity-prone (OP) group,obesity-resistant (OR)group and control (C)group.OP and OR rats continued to intake high-fat diet for 8 weeks,but those in control group were fed with normal diet (C-C)for 8 weeks.By applying the conditioned taste aversion (CTA)and the double-bottle experiment,we detected sucrose detection threshold and sucrose preference, and determined the total energy intake and feed efficiency.Results ① The weight gain of OP rats fed with high-fat diet was significantly higher than that of both OR rats and control rats,while it was significantly higher in the control rats than in OR rats.② The sucrose detection threshold of rats fed with high-fat diet (OP and OR)was significantly lower than that of rats in the control group fed with normal diet,but there was no difference between OP and OR rats.③ The sucrose preference of high-fat feeding rats (OP and OR)was significantly lower than that of rats in control group fed with normal diet,but there was no difference between OP and OR rats.④ The total caloric intake of OP rats fed with high-fat diet was significantly higher than that of high-fat feeding OR rats,but there was no difference between the high-fat feeding rats and the normal diet feeding rats.⑤ Food efficiency of high-fat feeding OP rats was significantly higher than that of OR rats fed with high-fat diet and control rats fed with normal diet,but there was no difference between high-fat feeding OR rats and normal diet feeding rats.Conclusion High-fat diet influences sweet taste sensitivity,which affects the feeding behavior and then results in obesity.