CAJ | 학술논문

目的：研究肌球蛋白Ⅱ（Myosin Ⅱ）对人卵巢癌细胞株增殖、迁移和侵袭能力的影响。方法分别采用real-time PCR和Western blot法检测卵巢癌中Myosin Ⅱ的mRNA及蛋白表达；在卵巢癌细胞中转染si-MyosinⅡ和Myosin，分别下调和过表达MyosinⅡ后，采用CCK-8法检测细胞的增殖，Matrigel小室法和Transwell小室法检测细胞的迁移和侵袭能力，Western blot检测Cyclin D1和MMP9蛋白的表达水平。结果 MyosinⅡ在卵巢癌组织中的表达显著高于正常卵巢组织，且与卵巢癌的临床分期有关（P<0.05）。在卵巢癌细胞株SKOV3和Hey中分别过表达和下调MyosinⅡ后，细胞的Cydin D1和MMP-9蛋白的表达也分别出现上调和下调，且细胞的增殖、迁移和侵袭能力均相应地增加或降低，与对照组比较差异显著（P<0.05）。结论 MyosinⅡ在卵巢癌中呈显著高表达，并可促进卵巢癌细胞的增殖、迁移及侵袭。
목적：연구기구단백Ⅱ（Myosin Ⅱ）대인란소암세포주증식、천이화침습능력적영향。방법분별채용real-time PCR화Western blot법검측란소암중Myosin Ⅱ적mRNA급단백표체；재란소암세포중전염si-MyosinⅡ화Myosin，분별하조화과표체MyosinⅡ후，채용CCK-8법검측세포적증식，Matrigel소실법화Transwell소실법검측세포적천이화침습능력，Western blot검측Cyclin D1화MMP9단백적표체수평。결과 MyosinⅡ재란소암조직중적표체현저고우정상란소조직，차여란소암적림상분기유관（P<0.05）。재란소암세포주SKOV3화Hey중분별과표체화하조MyosinⅡ후，세포적Cydin D1화MMP-9단백적표체야분별출현상조화하조，차세포적증식、천이화침습능력균상응지증가혹강저，여대조조비교차이현저（P<0.05）。결론 MyosinⅡ재란소암중정현저고표체，병가촉진란소암세포적증식、천이급침습。
Objective To investigate the expression of MyosinⅡin epithelial ovarian cancer (EOC) and it’s effects on pro-liferation, migration and invasion in EOC cells. Methods We detected the mRNA and protein expression of MyosinⅡin the in EOC tissue specimens by real-time polymerase chain reaction (PCR) and western blot respectively. After siRNA-mediated knockdown of MyosinⅡor lentivirus-mediated overexpression of MyosinⅡin EOC cell lines, CCK-8 kits were used to detect the proliferation of EOC cells. Matrigel and Transwell were used to observe cellular migration and invasion ability. Western blot were used to detect the protein expression levels of Cyclin D1 and matrix metalloproteinase-9 (MMP-9). Results Compared with normal ovarian tissues, MyosinⅡin EOC was highly expressed and correlated with the corresponding clinical stage (P<0.05). The capacity of proliferation, migration, invasion of EOC cells with down-regulated MyosinⅡwere decreased with statisti-cally significant differences, and the protein expression levels of Cyclin D1 and MMP-9 were also decreased, as compared with the control group (P<0.001). While overexpressed MyosinⅡincreased cell proliferation, migration and invasion, as well as the protein expression levels of Cyclin D1 and MMP-9, and the differences were statistically significant as compared with the control group (P<0.001). Conclusions MyosinⅡis highly expressed in EOC and promotes the proliferation, migration and migration capability of EOC cell.