CAJ | 학술논문

为研究投喂蚕豆对罗非鱼肝损伤及非特异性免疫功能的影响，本试验以浸泡蚕豆（试验组）、配合饲料（对照组）连续饲喂罗非鱼60 d，采样检测鱼血清中的肝损伤指标和非特异性免疫指标。结果显示：肝损伤情况，与对照组相比，试验组天冬氨酸转氨酶、丙氨酸转氨酶和腺苷脱氨酶活性分别提高13.67%、12.93%、19.12%（P＜0.05），乳酸脱氢酶活性变化不显著（P ＞0.05）；非特异性免疫功能方面，试验组的血清酸性磷酸酶和碱性磷酸酶活性降低22.96%、19.70%（P＜0.05），髓过氧化物酶和溶菌酶活性虽也有降低趋势，但差异不显著（P＞0.05）。因此，以蚕豆为饵料饲养罗非鱼，虽可脆化罗非鱼肉质，但却一定程度上造成鱼体肝脏损伤，降低鱼体非特异性免疫力。
위연구투위잠두대라비어간손상급비특이성면역공능적영향，본시험이침포잠두（시험조）、배합사료（대조조）련속사위라비어60 d，채양검측어혈청중적간손상지표화비특이성면역지표。결과현시：간손상정황，여대조조상비，시험조천동안산전안매、병안산전안매화선감탈안매활성분별제고13.67%、12.93%、19.12%（P＜0.05），유산탈경매활성변화불현저（P ＞0.05）；비특이성면역공능방면，시험조적혈청산성린산매화감성린산매활성강저22.96%、19.70%（P＜0.05），수과양화물매화용균매활성수야유강저추세，단차이불현저（P＞0.05）。인차，이잠두위이료사양라비어，수가취화라비어육질，단각일정정도상조성어체간장손상，강저어체비특이성면역력。
In order to study effects of broad bean on liver injury and non-specific immune responses activity of tilapia,Oreochromis niloticus were fed with broad bean and compound diet for 60 days.Liver injury and non-specific im-mune indexes were analyzed respectively.The results showed that liver injury indexes aspartate transaminase,alanine transaminase and adenosinase deaminase of test group were increased by 13.67%,12.93%,19.12%,compared with control group(P < 0.05),the activity of lactic dehydrogenase was not significantly affected (P > 0.05).The acid phosphatase and alkaline phosphate activity in test group were 22.96%,19.70%lower than those of control group(P<0.05).The myeloper-oxidase and lysozyme activity decreased slightly (P > 0.05).In conclusion,feed broad bean could embrittled the tilapia muscle obviously,but also lead to liver injury and reduce non-specific immune activity of Oreochromis niloticus.