CAJ | 학술논문

采用酶联免疫吸附（ELISA）方法检测约克夏猪和藏猪血清中脂联素和胰岛素的含量，并分析了其与肌内脂肪含量的关系。结果表明，约克夏猪和藏猪血清中脂联素含量分别为（62.59±5.51）μg/L和（87.79±1.97）μg/L，呈极显著差异（P<0.01）；胰岛素含量分别为（48.48±3.17） mIU/L和（47.41±0.94） mIU/L；肌内脂肪含量分别为（2.50±0.24）%和（5.48±0.44）%。相关性分析显示，脂联素水平与肌内脂肪含量呈极显著正相关（P<0.01），说明脂联素可通过血液正向调控肌内脂肪含量，初步阐明了猪血清中脂联素的品种差异及其与肌内脂肪含量的关系，为深入研究猪肌内脂肪沉积机理提供基础数据。
채용매련면역흡부（ELISA）방법검측약극하저화장저혈청중지련소화이도소적함량，병분석료기여기내지방함량적관계。결과표명，약극하저화장저혈청중지련소함량분별위（62.59±5.51）μg/L화（87.79±1.97）μg/L，정겁현저차이（P<0.01）；이도소함량분별위（48.48±3.17） mIU/L화（47.41±0.94） mIU/L；기내지방함량분별위（2.50±0.24）%화（5.48±0.44）%。상관성분석현시，지련소수평여기내지방함량정겁현저정상관（P<0.01），설명지련소가통과혈액정향조공기내지방함량，초보천명료저혈청중지련소적품충차이급기여기내지방함량적관계，위심입연구저기내지방침적궤리제공기출수거。
Detect the adiponectin and insulin content of Yorkshire and Tibetan pigs by enzyme-linked im-munosorbent assay(ELISA) and analyze its relationship with intramuscular fat content.The results showed that the adiponectin content of Yorkshire pigs and Tibetan pigs respectively were (62.59±5.51)μg/L and (87.79±1.97)μg/L, there was a significant difference (P<0.01);the insulin content respectively were(48.48±3.17) mIU/L and (47.41±0.94) mIU/L;intramuscular fat contents respectively were (2.50 ±0.24)% and (5.48 ±0.44)%.The correlation analysis showed that adiponectin and intramuscular fat content was significantly negatively correlated(P<0.01),adiponectin can positive regulation of intramuscular fat content by blood,which preliminary clarify the difference of adiponectin between varieties and the relationship with intramuscular fat content,it provide basic datas for re-searching deposition mechanism of pigs intramuscular fat content.