临床神经外科杂志
臨床神經外科雜誌
림상신경외과잡지
JOURNAL OF CLINICAL NEUROSURGERY
2015年
4期
274-278
,共5页
miR-150*%胶质瘤%exosome%增殖
miR-150*%膠質瘤%exosome%增殖
miR-150*%효질류%exosome%증식
miR-150*%glioma%exosome%proliferation
目的：研究 miR-150*修饰对骨髓间充质干细胞来源的囊泡（exosome）对胶质瘤细胞的影响。方法qRT-PCR 检测 miR-150*在胶质母细胞瘤组织与正常组织间的表达量差异。培养骨髓间充质干细胞（BMSCs），分别转染 miR-150*模拟物和阴性对照序列，上调 BMSCs 中 miR-150*表达水平，提取 BMSCs 培养基中的 exosome。Western blot 验证 exosomal 的表面标记蛋白 CD63和 flotillin-1，电镜下观察 exosome 的形态。CCK-8和细胞周期实验验证 miR-150*修饰 BMSCs 来源的 exosome 对胶质瘤细胞的影响。结果miR-150*在胶质母细胞瘤组织中表达明显低于正常脑组织，转染 miR-150*模拟物能明显提高 BMSCs 来源的 exosome 中miR-150*的表达。miR-150*修饰 BMSCs 来源的 exosome 能有效抑制胶质瘤细胞的增殖。结论 miR-150*（miR-150的互补核苷酸序列）在胶质母细胞瘤组织中低表达，miR-150*修饰 BMSCs 来源的 exosome 对胶质瘤细胞有抗增殖的作用。因此 exosome 可作为一种有效的基因治疗载体。
目的：研究 miR-150*脩飾對骨髓間充質榦細胞來源的囊泡（exosome）對膠質瘤細胞的影響。方法qRT-PCR 檢測 miR-150*在膠質母細胞瘤組織與正常組織間的錶達量差異。培養骨髓間充質榦細胞（BMSCs），分彆轉染 miR-150*模擬物和陰性對照序列，上調 BMSCs 中 miR-150*錶達水平，提取 BMSCs 培養基中的 exosome。Western blot 驗證 exosomal 的錶麵標記蛋白 CD63和 flotillin-1，電鏡下觀察 exosome 的形態。CCK-8和細胞週期實驗驗證 miR-150*脩飾 BMSCs 來源的 exosome 對膠質瘤細胞的影響。結果miR-150*在膠質母細胞瘤組織中錶達明顯低于正常腦組織，轉染 miR-150*模擬物能明顯提高 BMSCs 來源的 exosome 中miR-150*的錶達。miR-150*脩飾 BMSCs 來源的 exosome 能有效抑製膠質瘤細胞的增殖。結論 miR-150*（miR-150的互補覈苷痠序列）在膠質母細胞瘤組織中低錶達，miR-150*脩飾 BMSCs 來源的 exosome 對膠質瘤細胞有抗增殖的作用。因此 exosome 可作為一種有效的基因治療載體。
목적：연구 miR-150*수식대골수간충질간세포래원적낭포（exosome）대효질류세포적영향。방법qRT-PCR 검측 miR-150*재효질모세포류조직여정상조직간적표체량차이。배양골수간충질간세포（BMSCs），분별전염 miR-150*모의물화음성대조서렬，상조 BMSCs 중 miR-150*표체수평，제취 BMSCs 배양기중적 exosome。Western blot 험증 exosomal 적표면표기단백 CD63화 flotillin-1，전경하관찰 exosome 적형태。CCK-8화세포주기실험험증 miR-150*수식 BMSCs 래원적 exosome 대효질류세포적영향。결과miR-150*재효질모세포류조직중표체명현저우정상뇌조직，전염 miR-150*모의물능명현제고 BMSCs 래원적 exosome 중miR-150*적표체。miR-150*수식 BMSCs 래원적 exosome 능유효억제효질류세포적증식。결론 miR-150*（miR-150적호보핵감산서렬）재효질모세포류조직중저표체，miR-150*수식 BMSCs 래원적 exosome 대효질류세포유항증식적작용。인차 exosome 가작위일충유효적기인치료재체。
Objective MiRNA-based therapeutics hold great promise for tumor suppression, this study was to investigate the effect of miR-150*-loaded exosomes on regulation of glioma cells proliferation and cell cycle.Methods Quantitative real-time PCR on 15 glioblastoma tissues samples and normal controls were used to confirm the miR-150* expression level.Western blotting analysis and electron microscopy were employed to test exosomal biomarkers and their morphology. Transfection assay were used to collect miR-150*-loaded exosomes from bone marrow mesenchymal stem cells (BMSCs)culture medium.CCK-8 and cell cycle assays were used to analyze miR-150*-loaded exosomes effects on glioma cells.Results Level of miR-150* expression was much lower in glioblastoma than in normal tissues.Transfection assay successfully acquired miR150*-loaded exosomes which derived from bone marrow mesenchymal stem cells (BMSCs).Furthermore,miR-150*-loaded exosomes could largely inhibited glioma cells proliferation and suppress cell cycle progression.Cell counting kit 8 (CCK-8)assays also demonstrated miR-150* delivered in exosomes was much less toxic.Conclusions This study demonstrated miR-150* is down-regulated in glioblastoma.miR-150*-loaded exosomes could suppress glioma cells and exosomes may be a potentially efficient therapeutic delivery system.
