CAJ | 학술논문

目的探讨糖尿病肾病(diabetic nephropathy,DN)患者血浆差异蛋白表达情况,为寻找DN敏感血浆蛋白标志物提供科学依据.方法对6例DN患者的血浆与6例正常人血浆应用双向荧光差异凝胶电泳技术(2D-DIGE)观察疾病蛋白质标记物,选择表达差异大于1.5倍的蛋白斑点进行质谱分析.结果成功建立糖尿病肾病和正常血浆的胶内差异双向凝胶电泳图谱,通过分析共鉴定出1 1种差异蛋白质,包括C3补体、C4补体、载脂蛋白E等.结论 2D-DIGE能够客观地显示DN患者血浆与正常血浆之间的蛋白质表达差异,本研究鉴定的11种蛋白有可能为DN患者血浆蛋白分子标志物,可为DN的早期诊断提供依据.
목적 탐토당뇨병신병(diabetic nephropathy,DN)환자혈장차이단백표체정황,위심조DN민감혈장단백표지물제공과학의거.방법 대6례DN환자적혈장여6례정상인혈장응용쌍향형광차이응효전영기술(2D-DIGE)관찰질병단백질표기물,선택표체차이대우1.5배적단백반점진행질보분석.결과 성공건립당뇨병신병화정상혈장적효내차이쌍향응효전영도보,통과분석공감정출1 1충차이단백질,포괄C3보체、C4보체、재지단백E등.결론 2D-DIGE능구객관지현시DN환자혈장여정상혈장지간적단백질표체차이,본연구감정적11충단백유가능위DN환자혈장단백분자표지물,가위DN적조기진단제공의거.
Objective To investigate the expression of plasma proteins in patients with diabetic nephropathy (DN),to provide a scientific basis for searching for sensitive plasma protein markers in DN.Methods 6 patients with DN and 6 normal subjects were observed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) in plasma to study disease protein markers.Selected protein spots with differential expression was greater than 1.5 fold to give mass spectrometry analysis.Results A two-dimensional gel electrophoresis profile of diabetic nephropathy and normal plasma was successfully established.11 different proteins were identified by analysis,including C3,C4,Apolipoprotein E,etc.Conclusion 2D-DIGE can objectively show the difference of protein expression between DN patients and normal blood plasma.11 proteins identified in this study may be molecular markers for DN,can provide the basis for the early diagnosis of DN.