中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2015年
7期
470-475
,共6页
刘华%娄杰%刘晓英%尹晓燕%田字彬
劉華%婁傑%劉曉英%尹曉燕%田字彬
류화%루걸%류효영%윤효연%전자빈
微 RNA%微 RNA-21%微 RNA-146a%结直肠肿瘤%组织%血清%实时聚合酶链反应
微 RNA%微 RNA-21%微 RNA-146a%結直腸腫瘤%組織%血清%實時聚閤酶鏈反應
미 RNA%미 RNA-21%미 RNA-146a%결직장종류%조직%혈청%실시취합매련반응
microRNA%microRNA-21%microRNA-146a%Colorectal neoplasms%Tissues%Serum%Real-time polymerase chain reaction
目的探讨结直肠肿瘤患者组织和血清中 miRNA-21和 miRNA-146a 的表达情况，并分析其临床意义。方法收集100例结直肠癌、80例结直肠腺瘤患者和65名健康对照者的内镜活检组织，同时收集其血清标本，应用实时荧光定量 PCR 检测 miRNA-21和 miRNA-146a 的表达水平，分析其表达与临床病理特征间的关系，并评估血清差异表达的 miRNA 在结直肠肿瘤诊断中的价值。组间比较采用 Mann-WhitneyU 检验和 Kruskal-Wallis 检验；Spearman 相关性检验用于分析组织和血清中 miRNA 表达的关联性。结果在结直肠癌和结直肠腺瘤患者病变组织中 miRNA-21的表达分别为8．573±0．898和7．746±1．183，显著高于健康对照者的6．160±0．835（U ＝120．129、33．230，P 均＜0．01）；在结直肠癌和结直肠腺瘤患者血清中的表达分别为1．829±0．303和1．624±0．226，也高于健康对照者的1．391±0．221（U ＝40．353、15．512，P 均＜0．01）；miRNA-21在结直肠癌患者组织中和血清中的表达均高于结直肠腺瘤患者（U＝11．384、10．189，P 均＜0．01）。miRNA-21在结直肠癌患者中的高表达与肿瘤临床分期、淋巴结转移有关；miRNA-21在结直肠腺瘤中的表达水平与病理类型有关。Spearman 分析结果示，miRNA-21在结直肠癌患者组织和血清中的表达呈正相关（r ＝0．459，P ＜0．01）。miRNA-146a 在结直肠癌患者组织中为2．556±0．351，血清中为0．249±0．038，低于健康对照者的3．428±0．328和0．279±0．053（U ＝102．134、30．111，P 均＜0．01）；其在结直肠腺瘤患者组织中为3．255±0．332，血清中为0．290±0．036，与健康对照者比较差异无统计学意义（U＝3．936、3．180，P 均＞0．05）。miRNA-146a 在结直肠癌患者组织中和血清中的表达均低于结直肠腺瘤患者（U＝73．809、21．123，P 均＜0．01）。结直肠癌患者miRNA-146a 的下降程度与肿瘤临床分期、肿瘤分化程度有关，而在结直肠腺瘤中的表达与临床特征间无明显关系。根据 ROC 曲线分析，鉴别结直肠癌与健康者，血清 miRNA-21、miRNA-146a 及二者联合检测 ROC 的 AUC 分别为0．889、0．791和0．863；鉴别结直肠腺瘤与健康者，其 AUC 分别为0．784、0．692和0．761；鉴别结直肠癌与结直肠腺瘤患者，其 AUC 分别为0．705、0．820和0．713。结论血清 miRNA-21和 miRNA-146a 的差异表达在结直肠癌的早期诊断中具有潜在的价值。
目的探討結直腸腫瘤患者組織和血清中 miRNA-21和 miRNA-146a 的錶達情況，併分析其臨床意義。方法收集100例結直腸癌、80例結直腸腺瘤患者和65名健康對照者的內鏡活檢組織，同時收集其血清標本，應用實時熒光定量 PCR 檢測 miRNA-21和 miRNA-146a 的錶達水平，分析其錶達與臨床病理特徵間的關繫，併評估血清差異錶達的 miRNA 在結直腸腫瘤診斷中的價值。組間比較採用 Mann-WhitneyU 檢驗和 Kruskal-Wallis 檢驗；Spearman 相關性檢驗用于分析組織和血清中 miRNA 錶達的關聯性。結果在結直腸癌和結直腸腺瘤患者病變組織中 miRNA-21的錶達分彆為8．573±0．898和7．746±1．183，顯著高于健康對照者的6．160±0．835（U ＝120．129、33．230，P 均＜0．01）；在結直腸癌和結直腸腺瘤患者血清中的錶達分彆為1．829±0．303和1．624±0．226，也高于健康對照者的1．391±0．221（U ＝40．353、15．512，P 均＜0．01）；miRNA-21在結直腸癌患者組織中和血清中的錶達均高于結直腸腺瘤患者（U＝11．384、10．189，P 均＜0．01）。miRNA-21在結直腸癌患者中的高錶達與腫瘤臨床分期、淋巴結轉移有關；miRNA-21在結直腸腺瘤中的錶達水平與病理類型有關。Spearman 分析結果示，miRNA-21在結直腸癌患者組織和血清中的錶達呈正相關（r ＝0．459，P ＜0．01）。miRNA-146a 在結直腸癌患者組織中為2．556±0．351，血清中為0．249±0．038，低于健康對照者的3．428±0．328和0．279±0．053（U ＝102．134、30．111，P 均＜0．01）；其在結直腸腺瘤患者組織中為3．255±0．332，血清中為0．290±0．036，與健康對照者比較差異無統計學意義（U＝3．936、3．180，P 均＞0．05）。miRNA-146a 在結直腸癌患者組織中和血清中的錶達均低于結直腸腺瘤患者（U＝73．809、21．123，P 均＜0．01）。結直腸癌患者miRNA-146a 的下降程度與腫瘤臨床分期、腫瘤分化程度有關，而在結直腸腺瘤中的錶達與臨床特徵間無明顯關繫。根據 ROC 麯線分析，鑒彆結直腸癌與健康者，血清 miRNA-21、miRNA-146a 及二者聯閤檢測 ROC 的 AUC 分彆為0．889、0．791和0．863；鑒彆結直腸腺瘤與健康者，其 AUC 分彆為0．784、0．692和0．761；鑒彆結直腸癌與結直腸腺瘤患者，其 AUC 分彆為0．705、0．820和0．713。結論血清 miRNA-21和 miRNA-146a 的差異錶達在結直腸癌的早期診斷中具有潛在的價值。
목적탐토결직장종류환자조직화혈청중 miRNA-21화 miRNA-146a 적표체정황，병분석기림상의의。방법수집100례결직장암、80례결직장선류환자화65명건강대조자적내경활검조직，동시수집기혈청표본，응용실시형광정량 PCR 검측 miRNA-21화 miRNA-146a 적표체수평，분석기표체여림상병리특정간적관계，병평고혈청차이표체적 miRNA 재결직장종류진단중적개치。조간비교채용 Mann-WhitneyU 검험화 Kruskal-Wallis 검험；Spearman 상관성검험용우분석조직화혈청중 miRNA 표체적관련성。결과재결직장암화결직장선류환자병변조직중 miRNA-21적표체분별위8．573±0．898화7．746±1．183，현저고우건강대조자적6．160±0．835（U ＝120．129、33．230，P 균＜0．01）；재결직장암화결직장선류환자혈청중적표체분별위1．829±0．303화1．624±0．226，야고우건강대조자적1．391±0．221（U ＝40．353、15．512，P 균＜0．01）；miRNA-21재결직장암환자조직중화혈청중적표체균고우결직장선류환자（U＝11．384、10．189，P 균＜0．01）。miRNA-21재결직장암환자중적고표체여종류림상분기、림파결전이유관；miRNA-21재결직장선류중적표체수평여병리류형유관。Spearman 분석결과시，miRNA-21재결직장암환자조직화혈청중적표체정정상관（r ＝0．459，P ＜0．01）。miRNA-146a 재결직장암환자조직중위2．556±0．351，혈청중위0．249±0．038，저우건강대조자적3．428±0．328화0．279±0．053（U ＝102．134、30．111，P 균＜0．01）；기재결직장선류환자조직중위3．255±0．332，혈청중위0．290±0．036，여건강대조자비교차이무통계학의의（U＝3．936、3．180，P 균＞0．05）。miRNA-146a 재결직장암환자조직중화혈청중적표체균저우결직장선류환자（U＝73．809、21．123，P 균＜0．01）。결직장암환자miRNA-146a 적하강정도여종류림상분기、종류분화정도유관，이재결직장선류중적표체여림상특정간무명현관계。근거 ROC 곡선분석，감별결직장암여건강자，혈청 miRNA-21、miRNA-146a 급이자연합검측 ROC 적 AUC 분별위0．889、0．791화0．863；감별결직장선류여건강자，기 AUC 분별위0．784、0．692화0．761；감별결직장암여결직장선류환자，기 AUC 분별위0．705、0．820화0．713。결론혈청 miRNA-21화 miRNA-146a 적차이표체재결직장암적조기진단중구유잠재적개치。
Objective To investigate the differences of miRNA-21 and miRNA-146a expression between colorectal neoplasms tissues and serum of the patients with colorectal neoplasm,and the clinical significance was analyzed.Methods The endoscopic biopsy tissues and serum samples of 100 colorectal cancer (CRC),80 colorectal adenoma (CRA)patients and 65 healthy controls were collected.The expressions of miRNA-21 and miRNA-146a were detected by quantitative real time polymerase chain reaction.The relationship between the expression and clinicopathological features were analyzed.And then,the diagnostic value of expression difference of serum miRNA in colorectal neoplasm were evaluated. The Mann-WhitneyU test and Kruskal-Wallis test were performed for comparisons between groups,and the correlation of miRNA expression between tissues and serum was analyzed by Spearman test.Results <br> The expressions of miRNA-21 in the tissues of CRC and CRA were 8.573 ±0.898 and 7.746 ±1 .183, respectively,which were significantly higher than that of healthy controls 6.160 ±0.835 (U =120.129 and 33.230,both P <0.01).The expressions of miRNA-21 in the serum of CRC and CRA were 1 .829± 0.303 and 1 .624 ±0.226,respeotively,which were higher than that of healthy controls 1 .391 ±0.221 (U =40.353 and 15 .512,both P < 0.01 ).The expression of miRNA-21 in the serum and tissues of patients with CRC were both higher than those of patients with CRA (U =11 .384 and 10.189,both P <0.01).The expression of miRNA-21 in patients with CRC was associated with TNM stage and lymph node metastasis.The expression level of miRNA-21 in CRA was correlated with histological types.The results of Spearman analysis indicated that the expression of miRNA-21 in the tissues and serum of patients with CRC was positively correlated (r=0.459,P <0.01).The expression of miRNA-146a in the tissues and serum of CRC patients were 2.556±0.351 and 0.249±0.038,respectively,which were lower than those of healthy controls (3.428 ±0.328 and 0.279 ±0.053)(U =102.134 and 30.111 ,both P <0.01).The expression in the tissues and serum of CRA patients were 3.255 ±0.332 and 0.290±0.036, respectively,which were also lower than those of healthy controls,however the difference was not statistically significant (U = 3.936 and 3.180,both P > 0.05 ).The expression of miRNA-146a in the tissues and serum of CRC patients were both lower than those of CRA patients (U =73.809 and 21 .123, both P <0.01).The degree of decreased expression of miRNA-146a in CRC patients was correlated with TNM staging and tumor differentiation degree,however there was no correlation between the expression in CRA and clinical features.According to receiver operating characteristic (ROC)curve analysis,the AUC of miRNA-21 ,miRNA-146a and a combination of them in CRC and health individuals was 0.889, 0.791 and 0.863,respectively;in CRA and health individuals was 0.784,0.692 and 0.761 ,respectively;in CRC and CRA was 0.705 ,0.820 and 0.713,respectively.Conclusion The different expressions of miRNA-21 and miRNA-146a had potential values in early detection of colorectal cancer.
