CAJ | 학술논문

目的：通过过表达 miRNA -19b，研究 miRNA -19b 对 P19细胞分化的影响。方法脂质体2000转染miRNA -19b 过表达质粒与空载质粒进入 P19细胞，通过杀稻瘟菌素筛选出稳定株；实时定量聚合酶链反应检测 miRNA-19b 表达水平以及分化基因（cTnT、ANP、GATA4）的表达水平。结果 miRNA -19b 过表达载体与细胞系成功建立（ P ﹤0.05）；在 P19细胞分化过程中，miRNA -19b 过表达组心肌标志物均显著高于对照组（ P 均﹤0.05）。miRNA -19b显著促进 P19细胞向心肌细胞分化。结论 miRNA -19b 可以显著促进 P19细胞向心肌细胞的分化，为以后其用于心脏发育的进一步研究奠定基础。
목적：통과과표체 miRNA -19b，연구 miRNA -19b 대 P19세포분화적영향。방법지질체2000전염miRNA -19b 과표체질립여공재질립진입 P19세포，통과살도온균소사선출은정주；실시정량취합매련반응검측 miRNA-19b 표체수평이급분화기인（cTnT、ANP、GATA4）적표체수평。결과 miRNA -19b 과표체재체여세포계성공건립（ P ﹤0.05）；재 P19세포분화과정중，miRNA -19b 과표체조심기표지물균현저고우대조조（ P 균﹤0.05）。miRNA -19b현저촉진 P19세포향심기세포분화。결론 miRNA -19b 가이현저촉진 P19세포향심기세포적분화，위이후기용우심장발육적진일보연구전정기출。
Objective The aim of this study is to explore the effects of miRNA - 19b on P19 cell differentiation. Methods We trans-fected miRNA - 19b overexpression plasmid and empty plasmid into P19 cells by lipo2000. The stable cell lines was selected by blasticidin. The expression of miRNA - 19b and differentiation genes were detected by real - time quantitative PCR. Results The miRNA - 19b overexpression was successfully established( P ﹤ 0. 05). Compared with negative control group,the overexpression of miRNA - 19b promoted the differentiation of P19 cells significantly( P ﹤ 0. 05). Conclusion The miRNA - 19b significantly promotes the differentiation of P19 cells,which contribute to the further study of the heart development.