CAJ | 학술논문

目的：建立穿山龙配方颗粒质量标准。方法：采用薄层色谱法（TLC）对穿山龙配方颗粒进行定性鉴别；用高效液相色谱法（HPLC）对其中的薯蓣皂苷进行含量测定。结果：薄层色谱鉴别特征斑点清晰，专属性强；薯蓣皂苷进样量在0.6048～7.5600μg 与峰面积线性关系良好，r ＝0.9999，平均回收率为97.46％，RSD 为1.84％（n ＝6）。结论：本试验建立的定性及定量方法简单可行、重复性好，能有效地控制穿山龙配方颗粒的质量。
목적：건립천산룡배방과립질량표준。방법：채용박층색보법（TLC）대천산룡배방과립진행정성감별；용고효액상색보법（HPLC）대기중적서여조감진행함량측정。결과：박층색보감별특정반점청석，전속성강；서여조감진양량재0.6048～7.5600μg 여봉면적선성관계량호，r ＝0.9999，평균회수솔위97.46％，RSD 위1.84％（n ＝6）。결론：본시험건립적정성급정량방법간단가행、중복성호，능유효지공제천산룡배방과립적질량。
Objective:To establish quality standard for Rhizoma Dioscoreae Nipponicae formula granules.Methods:TLC was used for qualitative identification of Rhizoma Dioscoreae Nipponicae formula granules,the content of the main active constituent dioscin was determined by HPLC.Results: The characteristic spots in TLC identification were clear and specific.There was a good linear relationship between the peak area and quantity of dioscin at the range of 0.604 8-7.560 0 μg, r =0.999 9,and the average recovery rate was 97.46%,RSD was1.84% (n =6 ).Conclusion:By the study,the established qualitative and quantitative methods are simple and feasible,reproducible,and suitable for the qualitycontrol of Rhizoma Dioscoreae Nipponicae formula granules effectively.