CAJ | 학술논문

目的：探讨表皮生长因子受体（EGFR）基因在非小细胞肺癌（NSCLC）原发灶及淋巴结转移灶中的突变情况，分析其在不同病灶中的突变率与临床病理特征的关系。方法采用实时荧光聚合酶链反应（RT-PCR）的TaqMan探针法检测50例NSCLC原发灶及淋巴结转移灶中EGFR基因突变情况，并通过统计软件分析其与临床病理特征的相关性。结果50例NSCLC原发灶中，EGFR基因突变率为38.0%（19/50），与性别、病理组织学类型、吸烟关系密切（P<0.05），与年龄、分化程度、部位、民族、临床分期无关（P>0.05）；在淋巴结转移灶中的突变率为22.0%（11/50），与原发灶EGFR基因突变率比较差异有统计学意义（P<0.05），而与其他临床病理特征无关（P>0.05）。结论 EGFR基因突变主要集中于女性、肺腺癌、非吸烟患者，并且在NSCLC原发灶及淋巴结转移灶中突变不一致，表现出肿瘤的异质性，影响疗效。
목적：탐토표피생장인자수체（EGFR）기인재비소세포폐암（NSCLC）원발조급림파결전이조중적돌변정황，분석기재불동병조중적돌변솔여림상병리특정적관계。방법채용실시형광취합매련반응（RT-PCR）적TaqMan탐침법검측50례NSCLC원발조급림파결전이조중EGFR기인돌변정황，병통과통계연건분석기여림상병리특정적상관성。결과50례NSCLC원발조중，EGFR기인돌변솔위38.0%（19/50），여성별、병리조직학류형、흡연관계밀절（P<0.05），여년령、분화정도、부위、민족、림상분기무관（P>0.05）；재림파결전이조중적돌변솔위22.0%（11/50），여원발조EGFR기인돌변솔비교차이유통계학의의（P<0.05），이여기타림상병리특정무관（P>0.05）。결론 EGFR기인돌변주요집중우녀성、폐선암、비흡연환자，병차재NSCLC원발조급림파결전이조중돌변불일치，표현출종류적이질성，영향료효。
Objective To investigate the mutations situation of epidermal growth factor receptor (EGFR) gene in primary focus and metastatic focus of non-small cell lung cancer (NSCLC), and to analyze the relationship of the mutation rate of it in different focus and clinicopathologic feature. Methods The TaqMan probe method of real-time polymerase chain reaction (RT-PCR) was used to detect the conditions of EGFR gene mutation in primary focus and metastatic focus of 50 cases with NSCLC, and its correlation with clinicopathologic feature was analyzed through statistical software. Results Among the primary focus of 50 cases with NSCLC, the gene mutation rate of EGFR was 38.0%(19/50), which was closely correlated with gender, histopathological type, smoking (P<0.05), which had no correlations with age, differentiated degree, postion, nation, clinical stages (P>0.05);the mutation rate in metastatic focus was 22.0%(11/50), which had a statistically significant difference compared with gene mutation rate of EGFR in primary focus (P<0.05), while it had no correlations with other clinicopathologic features (P>0.05). Conclusion The gene mutation of EGFR was mainly concentrated on female, pulmonary adenocarcinoma, non-smoking patients, and shows different mutation in primary focus and metastatic focus of NSCLC, put up the heterogeneity of tumor, which affects the curative effect.